Potential Role of Phytochromes A and B and Cryptochrome 1 in the Adaptation of <i>Solanum lycopersicum</i> to UV-B Radiation
UV-B causes both damage to the photosynthetic apparatus (PA) and the activation of specific mechanisms that protect the PA from excess energy and trigger a cascade of regulatory interactions with different photoreceptors, including phytochromes (PHYs) and cryptochromes (CRYs). However, the role of p...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/17/13142 |
| _version_ | 1797582456481644544 |
|---|---|
| author | Anna Abramova Mikhail Vereshchagin Leonid Kulkov Vladimir D. Kreslavski Vladimir V. Kuznetsov Pavel Pashkovskiy |
| author_facet | Anna Abramova Mikhail Vereshchagin Leonid Kulkov Vladimir D. Kreslavski Vladimir V. Kuznetsov Pavel Pashkovskiy |
| author_sort | Anna Abramova |
| collection | DOAJ |
| description | UV-B causes both damage to the photosynthetic apparatus (PA) and the activation of specific mechanisms that protect the PA from excess energy and trigger a cascade of regulatory interactions with different photoreceptors, including phytochromes (PHYs) and cryptochromes (CRYs). However, the role of photoreceptors in plants’ responses to UV-B radiation remains undiscovered. This study explores some of these responses using tomato photoreceptor mutants (<i>phya</i>, <i>phyb1</i>, <i>phyab2</i>, <i>cry1</i>). The effects of UV-B exposure (12.3 µmol (photons) m<sup>−2</sup> s<sup>−1</sup>) on photosynthetic rates and PSII photochemical activity, the contents of photosynthetic and UV-absorbing pigments and anthocyanins, and the nonenzymatic antioxidant capacity (TEAC) were studied. The expression of key light-signaling genes, including UV-B signaling and genes associated with the biosynthesis of chlorophylls, carotenoids, anthocyanins, and flavonoids, was also determined. Under UV-B, <i>phyab2</i> and <i>cry1</i> mutants demonstrated a reduction in the PSII effective quantum yield and photosynthetic rate, as well as a reduced value of TEAC. At the same time, UV-B irradiation led to a noticeable decrease in the expression of the <i>ultraviolet-B receptor (UVR8), repressor of UV-B photomorphogenesis 2 (RUP2), cullin 4 (CUL4), anthocyanidin synthase (ANT), phenylalanine ammonia-lease (PAL)</i>, and <i>phytochrome B2</i> (<i>PHYB2)</i> genes in <i>phyab2</i> and <i>RUP2, CUL4, ANT, PAL</i>, and <i>elongated hypocotyl 5 (HY5)</i> genes in the <i>cry1</i> mutant. The results indicate the mutual regulation of UVR8, PHYB2, and CRY1 photoreceptors, but not PHYB1 and PHYA, in the process of forming a response to UV-B irradiation in tomato. |
| first_indexed | 2024-03-10T23:22:35Z |
| format | Article |
| id | doaj.art-74257474c8014dd784997dabe8ee185e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:22:35Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-74257474c8014dd784997dabe8ee185e2023-11-19T08:13:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124171314210.3390/ijms241713142Potential Role of Phytochromes A and B and Cryptochrome 1 in the Adaptation of <i>Solanum lycopersicum</i> to UV-B RadiationAnna Abramova0Mikhail Vereshchagin1Leonid Kulkov2Vladimir D. Kreslavski3Vladimir V. Kuznetsov4Pavel Pashkovskiy5K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, RussiaDepartment of Technologies for the Production of Vegetable, Medicinal and Essential Oils, Russian State Agrarian University, Moscow Timiryazev Agricultural Academy, Timiryazevskaya Street 49, Moscow 127550, RussiaInstitute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino 142290, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, RussiaUV-B causes both damage to the photosynthetic apparatus (PA) and the activation of specific mechanisms that protect the PA from excess energy and trigger a cascade of regulatory interactions with different photoreceptors, including phytochromes (PHYs) and cryptochromes (CRYs). However, the role of photoreceptors in plants’ responses to UV-B radiation remains undiscovered. This study explores some of these responses using tomato photoreceptor mutants (<i>phya</i>, <i>phyb1</i>, <i>phyab2</i>, <i>cry1</i>). The effects of UV-B exposure (12.3 µmol (photons) m<sup>−2</sup> s<sup>−1</sup>) on photosynthetic rates and PSII photochemical activity, the contents of photosynthetic and UV-absorbing pigments and anthocyanins, and the nonenzymatic antioxidant capacity (TEAC) were studied. The expression of key light-signaling genes, including UV-B signaling and genes associated with the biosynthesis of chlorophylls, carotenoids, anthocyanins, and flavonoids, was also determined. Under UV-B, <i>phyab2</i> and <i>cry1</i> mutants demonstrated a reduction in the PSII effective quantum yield and photosynthetic rate, as well as a reduced value of TEAC. At the same time, UV-B irradiation led to a noticeable decrease in the expression of the <i>ultraviolet-B receptor (UVR8), repressor of UV-B photomorphogenesis 2 (RUP2), cullin 4 (CUL4), anthocyanidin synthase (ANT), phenylalanine ammonia-lease (PAL)</i>, and <i>phytochrome B2</i> (<i>PHYB2)</i> genes in <i>phyab2</i> and <i>RUP2, CUL4, ANT, PAL</i>, and <i>elongated hypocotyl 5 (HY5)</i> genes in the <i>cry1</i> mutant. The results indicate the mutual regulation of UVR8, PHYB2, and CRY1 photoreceptors, but not PHYB1 and PHYA, in the process of forming a response to UV-B irradiation in tomato.https://www.mdpi.com/1422-0067/24/17/13142tomatophotoreceptor mutantsstress resistancephotosynthesisUV-B |
| spellingShingle | Anna Abramova Mikhail Vereshchagin Leonid Kulkov Vladimir D. Kreslavski Vladimir V. Kuznetsov Pavel Pashkovskiy Potential Role of Phytochromes A and B and Cryptochrome 1 in the Adaptation of <i>Solanum lycopersicum</i> to UV-B Radiation International Journal of Molecular Sciences tomato photoreceptor mutants stress resistance photosynthesis UV-B |
| title | Potential Role of Phytochromes A and B and Cryptochrome 1 in the Adaptation of <i>Solanum lycopersicum</i> to UV-B Radiation |
| title_full | Potential Role of Phytochromes A and B and Cryptochrome 1 in the Adaptation of <i>Solanum lycopersicum</i> to UV-B Radiation |
| title_fullStr | Potential Role of Phytochromes A and B and Cryptochrome 1 in the Adaptation of <i>Solanum lycopersicum</i> to UV-B Radiation |
| title_full_unstemmed | Potential Role of Phytochromes A and B and Cryptochrome 1 in the Adaptation of <i>Solanum lycopersicum</i> to UV-B Radiation |
| title_short | Potential Role of Phytochromes A and B and Cryptochrome 1 in the Adaptation of <i>Solanum lycopersicum</i> to UV-B Radiation |
| title_sort | potential role of phytochromes a and b and cryptochrome 1 in the adaptation of i solanum lycopersicum i to uv b radiation |
| topic | tomato photoreceptor mutants stress resistance photosynthesis UV-B |
| url | https://www.mdpi.com/1422-0067/24/17/13142 |
| work_keys_str_mv | AT annaabramova potentialroleofphytochromesaandbandcryptochrome1intheadaptationofisolanumlycopersicumitouvbradiation AT mikhailvereshchagin potentialroleofphytochromesaandbandcryptochrome1intheadaptationofisolanumlycopersicumitouvbradiation AT leonidkulkov potentialroleofphytochromesaandbandcryptochrome1intheadaptationofisolanumlycopersicumitouvbradiation AT vladimirdkreslavski potentialroleofphytochromesaandbandcryptochrome1intheadaptationofisolanumlycopersicumitouvbradiation AT vladimirvkuznetsov potentialroleofphytochromesaandbandcryptochrome1intheadaptationofisolanumlycopersicumitouvbradiation AT pavelpashkovskiy potentialroleofphytochromesaandbandcryptochrome1intheadaptationofisolanumlycopersicumitouvbradiation |