Expression profiling and characterization of a G-Box binding protein, B12Dg, from pearl millet
Objectives: This study aims to characterize the B12Dg from pearl millet and assess its expression in response to abiotic stress and plant hormones during germination. Methods: cDNA of B12Dg has been sequenced, the deduced protein has been characterized through in silico tools, and B12Dg expression h...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722006292 |
| _version_ | 1811199066489487360 |
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| author | Zainab M. Almutairi |
| author_facet | Zainab M. Almutairi |
| author_sort | Zainab M. Almutairi |
| collection | DOAJ |
| description | Objectives: This study aims to characterize the B12Dg from pearl millet and assess its expression in response to abiotic stress and plant hormones during germination. Methods: cDNA of B12Dg has been sequenced, the deduced protein has been characterized through in silico tools, and B12Dg expression has been examined after treatment by the plant hormones and under abiotic stresses. Results: The sequenced cDNA for PgB12Dg consisted of 314 base pairs and had an open reading frame of 261 base pairs encoded for 87 amino acids. The deduced PgB12Dg protein comprised a B12D domain from 7 to 74 amino acids and a transmembrane helix. Strong conservation was observed among the PgB12Dg protein and its orthologues from monocots. Transmembrane topology prediction and subcellular localization suggested that the deduced PgB12Dg protein accommodates transmembrane helix spanning from 10 to 32 amino acids embedded in the inner mitochondrion membrane. Expression analysis by real-time PCR indicated that PgB12Dg was upregulated in roots but downregulated in seeds from 3 day-old-seedlings under drought and cold stress. At the same time, no significant up or downregulation was shown under salt stress. Treatment by plant hormones showed upregulation by gibberellic acid and downregulation by abscisic acid in one-day-old germinated seeds. Conclusions: The regulation of PgB12Dg by plant hormones and abiotic stress in pearl millet during germination can help to understand the transcriptional regulation mechanisms of the PgB12Dg during plant growth under stress. |
| first_indexed | 2024-04-12T01:41:38Z |
| format | Article |
| id | doaj.art-7425d5c4e06241ccb779ec5c5ca211f0 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-12T01:41:38Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-7425d5c4e06241ccb779ec5c5ca211f02022-12-22T03:53:11ZengElsevierJournal of King Saud University: Science1018-36472023-01-01351102448Expression profiling and characterization of a G-Box binding protein, B12Dg, from pearl milletZainab M. Almutairi0Department of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam bin Abdulaziz University, P.O. Box: 83, Al-kharj 11942, Saudi ArabiaObjectives: This study aims to characterize the B12Dg from pearl millet and assess its expression in response to abiotic stress and plant hormones during germination. Methods: cDNA of B12Dg has been sequenced, the deduced protein has been characterized through in silico tools, and B12Dg expression has been examined after treatment by the plant hormones and under abiotic stresses. Results: The sequenced cDNA for PgB12Dg consisted of 314 base pairs and had an open reading frame of 261 base pairs encoded for 87 amino acids. The deduced PgB12Dg protein comprised a B12D domain from 7 to 74 amino acids and a transmembrane helix. Strong conservation was observed among the PgB12Dg protein and its orthologues from monocots. Transmembrane topology prediction and subcellular localization suggested that the deduced PgB12Dg protein accommodates transmembrane helix spanning from 10 to 32 amino acids embedded in the inner mitochondrion membrane. Expression analysis by real-time PCR indicated that PgB12Dg was upregulated in roots but downregulated in seeds from 3 day-old-seedlings under drought and cold stress. At the same time, no significant up or downregulation was shown under salt stress. Treatment by plant hormones showed upregulation by gibberellic acid and downregulation by abscisic acid in one-day-old germinated seeds. Conclusions: The regulation of PgB12Dg by plant hormones and abiotic stress in pearl millet during germination can help to understand the transcriptional regulation mechanisms of the PgB12Dg during plant growth under stress.http://www.sciencedirect.com/science/article/pii/S1018364722006292Pennisetum glaucumB12DgcDNAGene expression |
| spellingShingle | Zainab M. Almutairi Expression profiling and characterization of a G-Box binding protein, B12Dg, from pearl millet Journal of King Saud University: Science Pennisetum glaucum B12Dg cDNA Gene expression |
| title | Expression profiling and characterization of a G-Box binding protein, B12Dg, from pearl millet |
| title_full | Expression profiling and characterization of a G-Box binding protein, B12Dg, from pearl millet |
| title_fullStr | Expression profiling and characterization of a G-Box binding protein, B12Dg, from pearl millet |
| title_full_unstemmed | Expression profiling and characterization of a G-Box binding protein, B12Dg, from pearl millet |
| title_short | Expression profiling and characterization of a G-Box binding protein, B12Dg, from pearl millet |
| title_sort | expression profiling and characterization of a g box binding protein b12dg from pearl millet |
| topic | Pennisetum glaucum B12Dg cDNA Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722006292 |
| work_keys_str_mv | AT zainabmalmutairi expressionprofilingandcharacterizationofagboxbindingproteinb12dgfrompearlmillet |