A bioluminescence reporter mouse that monitors expression of constitutively active β-catenin.
This short technical report describes the generation and characterization of a bioluminescence reporter mouse that is engineered to detect and longitudinally monitor the expression of doxycycline-induced constitutively active β-catenin. The new responder transgenic mouse contains the TetO-ΔN89β-CatT...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5333872?pdf=render |
| _version_ | 1819091328044105728 |
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| author | Maria M Szwarc Ramakrishna Kommagani Mary C Peavey Lan Hai David M Lonard John P Lydon |
| author_facet | Maria M Szwarc Ramakrishna Kommagani Mary C Peavey Lan Hai David M Lonard John P Lydon |
| author_sort | Maria M Szwarc |
| collection | DOAJ |
| description | This short technical report describes the generation and characterization of a bioluminescence reporter mouse that is engineered to detect and longitudinally monitor the expression of doxycycline-induced constitutively active β-catenin. The new responder transgenic mouse contains the TetO-ΔN89β-CatTMILA transgene, which consists of the tet-operator followed by a bicistronic sequence encoding a stabilized form of active β-catenin (ΔN89β-catenin), an internal ribosome entry site, and the firefly luciferase gene. To confirm that the transgene operates as designed, TetO-ΔN89β-CatTMILA transgenic mouse lines were crossed with an effector mouse that harbors the mouse mammary tumor virus-reverse tetracycline transactivator (MMTV-rtTA) transgene (termed MTB hereon), which primarily targets rtTA expression to the mammary epithelium. Following doxycycline administration, the resultant MTB/CatTMILA bigenic reporter exhibited precocious lobuloalveologenesis, ductal hyperplasia, and mammary adenocarcinomas, which were visualized and monitored by in vivo bioluminescence detection. Therefore, we predict that the TetO-ΔN89β-CatTMILA transgenic responder mouse-when crossed with the appropriate effector transgenic-will have wide-applicability to non-invasively monitor the influence of constitutively active β-catenin expression on cell-fate specification, proliferation, differentiation, and neoplastic transformation in a broad spectrum of target tissues. |
| first_indexed | 2024-12-21T22:37:58Z |
| format | Article |
| id | doaj.art-74294098c1c740dd89fc6c10645d9c92 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T22:37:58Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-74294098c1c740dd89fc6c10645d9c922022-12-21T18:47:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017301410.1371/journal.pone.0173014A bioluminescence reporter mouse that monitors expression of constitutively active β-catenin.Maria M SzwarcRamakrishna KommaganiMary C PeaveyLan HaiDavid M LonardJohn P LydonThis short technical report describes the generation and characterization of a bioluminescence reporter mouse that is engineered to detect and longitudinally monitor the expression of doxycycline-induced constitutively active β-catenin. The new responder transgenic mouse contains the TetO-ΔN89β-CatTMILA transgene, which consists of the tet-operator followed by a bicistronic sequence encoding a stabilized form of active β-catenin (ΔN89β-catenin), an internal ribosome entry site, and the firefly luciferase gene. To confirm that the transgene operates as designed, TetO-ΔN89β-CatTMILA transgenic mouse lines were crossed with an effector mouse that harbors the mouse mammary tumor virus-reverse tetracycline transactivator (MMTV-rtTA) transgene (termed MTB hereon), which primarily targets rtTA expression to the mammary epithelium. Following doxycycline administration, the resultant MTB/CatTMILA bigenic reporter exhibited precocious lobuloalveologenesis, ductal hyperplasia, and mammary adenocarcinomas, which were visualized and monitored by in vivo bioluminescence detection. Therefore, we predict that the TetO-ΔN89β-CatTMILA transgenic responder mouse-when crossed with the appropriate effector transgenic-will have wide-applicability to non-invasively monitor the influence of constitutively active β-catenin expression on cell-fate specification, proliferation, differentiation, and neoplastic transformation in a broad spectrum of target tissues.http://europepmc.org/articles/PMC5333872?pdf=render |
| spellingShingle | Maria M Szwarc Ramakrishna Kommagani Mary C Peavey Lan Hai David M Lonard John P Lydon A bioluminescence reporter mouse that monitors expression of constitutively active β-catenin. PLoS ONE |
| title | A bioluminescence reporter mouse that monitors expression of constitutively active β-catenin. |
| title_full | A bioluminescence reporter mouse that monitors expression of constitutively active β-catenin. |
| title_fullStr | A bioluminescence reporter mouse that monitors expression of constitutively active β-catenin. |
| title_full_unstemmed | A bioluminescence reporter mouse that monitors expression of constitutively active β-catenin. |
| title_short | A bioluminescence reporter mouse that monitors expression of constitutively active β-catenin. |
| title_sort | bioluminescence reporter mouse that monitors expression of constitutively active β catenin |
| url | http://europepmc.org/articles/PMC5333872?pdf=render |
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