Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro
Doxorubicin (Dox) is a potent anti-cancer agent with cardiotoxic side-effects but the mechanism of its cardiotoxicity and its effect on expression of the vasoactive atrial natriuretic peptide (ANP), an important marker for cardiac hypertrophy, are little understood. The present study examined Dox-in...
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| Format: | Article |
| Language: | English |
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BMC
2001-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300007 |
| _version_ | 1819010911755567104 |
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| author | ASIM RAHMAN MAHMOOD ALAM SUDHA RAO LIN CAI CLARK LUTHER T. SAYID SHAFIQ M.A.Q SIDDIQUI |
| author_facet | ASIM RAHMAN MAHMOOD ALAM SUDHA RAO LIN CAI CLARK LUTHER T. SAYID SHAFIQ M.A.Q SIDDIQUI |
| author_sort | ASIM RAHMAN |
| collection | DOAJ |
| description | Doxorubicin (Dox) is a potent anti-cancer agent with cardiotoxic side-effects but the mechanism of its cardiotoxicity and its effect on expression of the vasoactive atrial natriuretic peptide (ANP), an important marker for cardiac hypertrophy, are little understood. The present study examined Dox-induced changes in vivo in hearts of 6 mongrel dogs and 5 Sprague-Dawley rats and in vitro in cardiac cultures of neonatal rats. Quantitative RT-PCR analysis using g32-p labeled primers for ß-actin, phospholamban (PLB) and ANP showed a selective 5-fold increase of ANP mRNA in Dox-treated dog hearts in comparison to controls. Similarly, northern analysis of GAPD, ß-actin, cardiac a-actin and ANP gave a selective 4.5-fold increase in ANP transcripts in Dox-treated rat hearts. On the other hand, there was a selective decrease (approximately 39%) of ANP transcripts in Dox-treated cardiac cultures relative to controls. Immunohistochemistry localized the ANP changes both in tissue sections and in cultures to the cardiomyocytes. The data clearly showed that Dox selectively increases ANP expression in dog and rat hearts in absence of cardiocyte hypertrophy but selectively decreases it in cardiac cultures. This differential effect of Dox on cardiocytes in vivo and in vitro should be a useful parameter for studies of transcriptional control of ANP expression. |
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| id | doaj.art-7429bbfc8fec46a3a44ebcb3180ed2f4 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-21T01:19:47Z |
| publishDate | 2001-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-7429bbfc8fec46a3a44ebcb3180ed2f42022-12-21T19:20:41ZengBMCBiological Research0716-97600717-62872001-01-01343-4195206Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitroASIM RAHMANMAHMOOD ALAMSUDHA RAOLIN CAICLARK LUTHER T.SAYID SHAFIQM.A.Q SIDDIQUIDoxorubicin (Dox) is a potent anti-cancer agent with cardiotoxic side-effects but the mechanism of its cardiotoxicity and its effect on expression of the vasoactive atrial natriuretic peptide (ANP), an important marker for cardiac hypertrophy, are little understood. The present study examined Dox-induced changes in vivo in hearts of 6 mongrel dogs and 5 Sprague-Dawley rats and in vitro in cardiac cultures of neonatal rats. Quantitative RT-PCR analysis using g32-p labeled primers for ß-actin, phospholamban (PLB) and ANP showed a selective 5-fold increase of ANP mRNA in Dox-treated dog hearts in comparison to controls. Similarly, northern analysis of GAPD, ß-actin, cardiac a-actin and ANP gave a selective 4.5-fold increase in ANP transcripts in Dox-treated rat hearts. On the other hand, there was a selective decrease (approximately 39%) of ANP transcripts in Dox-treated cardiac cultures relative to controls. Immunohistochemistry localized the ANP changes both in tissue sections and in cultures to the cardiomyocytes. The data clearly showed that Dox selectively increases ANP expression in dog and rat hearts in absence of cardiocyte hypertrophy but selectively decreases it in cardiac cultures. This differential effect of Dox on cardiocytes in vivo and in vitro should be a useful parameter for studies of transcriptional control of ANP expression.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300007Atrial natriuretic peptidegene expressionadriamycinheart failure |
| spellingShingle | ASIM RAHMAN MAHMOOD ALAM SUDHA RAO LIN CAI CLARK LUTHER T. SAYID SHAFIQ M.A.Q SIDDIQUI Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro Biological Research Atrial natriuretic peptide gene expression adriamycin heart failure |
| title | Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro |
| title_full | Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro |
| title_fullStr | Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro |
| title_full_unstemmed | Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro |
| title_short | Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro |
| title_sort | differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro |
| topic | Atrial natriuretic peptide gene expression adriamycin heart failure |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300007 |
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