Effects of nisin on the expression of virulence genes of methicillin-resistant/sensitive Staphylococcus aureus
Background and Objectives: Few studies have considered potential benefits of probiotic bacteria and their derivatives on human and animal health. Nisin is an antimicrobial agent that is produced by lactobacilli and served as a preservative in foods. This study aims to investigate whether nisin supp...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-12-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/2921 |
| _version_ | 1811195265978204160 |
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| author | Mahnaz Ramezani Ebrahim Rezazadeh Zarandi Nahid Zainodini Reza Bahramabadi Shokrollah Assar |
| author_facet | Mahnaz Ramezani Ebrahim Rezazadeh Zarandi Nahid Zainodini Reza Bahramabadi Shokrollah Assar |
| author_sort | Mahnaz Ramezani |
| collection | DOAJ |
| description |
Background and Objectives: Few studies have considered potential benefits of probiotic bacteria and their derivatives on human and animal health. Nisin is an antimicrobial agent that is produced by lactobacilli and served as a preservative in foods. This study aims to investigate whether nisin suppresses or decreases the genes involved in the pathogenicity of methicillin-susceptible and methicillin-resistant Staphylococcus aureus (MSSA and MRSA).
Materials and Methods: MSSA and MRSA strains were cultured at the ¼, ½, and 1 × minimum inhibitory concentration (MIC) of nisin. Next, RNA extraction was performed at the mid-exponential stage of growth, and cDNA was synthesized. The expression of virulence factors was measured by qPCR, and the data were analyzed by the ΔΔCt formula.
Results: Depending on the incubation times and the Lactobacillus species, the MIC of nisin on MRSA and MSSA observed in 800 and 1600 mg/l, respectively. The qPCR assay showed the expression level of the sea, agrA, and spa genes decreased and the level of the sae gene increased at the sub-MIC of nisin, and no antagonism was observed. Concerning MRSA, the maximum downregulation rate was observed in the sea gene (up to 5.9 folds) while in MSSA, the maximum downregulation rate was noticed in the agrA gene (up to 10 folds).
Conclusion: Due to the high inhibitory effect of the sub-MIC of nisin on the expression of virulence factor genes in MRSA and MSSA, this compound could potentially reduce the virulence of S. aureus.
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| first_indexed | 2024-04-12T00:40:31Z |
| format | Article |
| id | doaj.art-742cb14743864cc4b0e20707ba780eb8 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-12T00:40:31Z |
| publishDate | 2022-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-742cb14743864cc4b0e20707ba780eb82022-12-22T03:55:02ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472022-12-0114610.18502/ijm.v14i6.11262Effects of nisin on the expression of virulence genes of methicillin-resistant/sensitive Staphylococcus aureusMahnaz Ramezani0Ebrahim Rezazadeh Zarandi1Nahid Zainodini2Reza Bahramabadi3Shokrollah Assar4Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, IranImmunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran; Department of Microbiology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, IranImmunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, IranDepartment of Microbiology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, IranImmunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran; Department of Microbiology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Background and Objectives: Few studies have considered potential benefits of probiotic bacteria and their derivatives on human and animal health. Nisin is an antimicrobial agent that is produced by lactobacilli and served as a preservative in foods. This study aims to investigate whether nisin suppresses or decreases the genes involved in the pathogenicity of methicillin-susceptible and methicillin-resistant Staphylococcus aureus (MSSA and MRSA). Materials and Methods: MSSA and MRSA strains were cultured at the ¼, ½, and 1 × minimum inhibitory concentration (MIC) of nisin. Next, RNA extraction was performed at the mid-exponential stage of growth, and cDNA was synthesized. The expression of virulence factors was measured by qPCR, and the data were analyzed by the ΔΔCt formula. Results: Depending on the incubation times and the Lactobacillus species, the MIC of nisin on MRSA and MSSA observed in 800 and 1600 mg/l, respectively. The qPCR assay showed the expression level of the sea, agrA, and spa genes decreased and the level of the sae gene increased at the sub-MIC of nisin, and no antagonism was observed. Concerning MRSA, the maximum downregulation rate was observed in the sea gene (up to 5.9 folds) while in MSSA, the maximum downregulation rate was noticed in the agrA gene (up to 10 folds). Conclusion: Due to the high inhibitory effect of the sub-MIC of nisin on the expression of virulence factor genes in MRSA and MSSA, this compound could potentially reduce the virulence of S. aureus. https://ijm.tums.ac.ir/index.php/ijm/article/view/2921Nisin;Methicillin-resistant Staphylococcus aureus;Methicillin-sensitive Staphylococcus aureus;Virulence factor;Food-borne |
| spellingShingle | Mahnaz Ramezani Ebrahim Rezazadeh Zarandi Nahid Zainodini Reza Bahramabadi Shokrollah Assar Effects of nisin on the expression of virulence genes of methicillin-resistant/sensitive Staphylococcus aureus Iranian Journal of Microbiology Nisin; Methicillin-resistant Staphylococcus aureus; Methicillin-sensitive Staphylococcus aureus; Virulence factor; Food-borne |
| title | Effects of nisin on the expression of virulence genes of methicillin-resistant/sensitive Staphylococcus aureus |
| title_full | Effects of nisin on the expression of virulence genes of methicillin-resistant/sensitive Staphylococcus aureus |
| title_fullStr | Effects of nisin on the expression of virulence genes of methicillin-resistant/sensitive Staphylococcus aureus |
| title_full_unstemmed | Effects of nisin on the expression of virulence genes of methicillin-resistant/sensitive Staphylococcus aureus |
| title_short | Effects of nisin on the expression of virulence genes of methicillin-resistant/sensitive Staphylococcus aureus |
| title_sort | effects of nisin on the expression of virulence genes of methicillin resistant sensitive staphylococcus aureus |
| topic | Nisin; Methicillin-resistant Staphylococcus aureus; Methicillin-sensitive Staphylococcus aureus; Virulence factor; Food-borne |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/2921 |
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