Enhanced Fermentative Hydrogen Production from Food Waste in Continuous Reactor after Butyric Acid Treatment
End-product accumulation during dark fermentation leads to process instability and hydrogen production inhibition. To overcome this constraint, microbial community adaptation to butyric acid can induce acid tolerance and thus enhance the hydrogen yields; however, adaptation and selection of appropri...
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MDPI AG
2022-05-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/15/11/4048 |
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| author | Marie Céline Noguer Jose Antonio Magdalena Nicolas Bernet Renaud Escudié Eric Trably |
| author_facet | Marie Céline Noguer Jose Antonio Magdalena Nicolas Bernet Renaud Escudié Eric Trably |
| author_sort | Marie Céline Noguer |
| collection | DOAJ |
| description | End-product accumulation during dark fermentation leads to process instability and hydrogen production inhibition. To overcome this constraint, microbial community adaptation to butyric acid can induce acid tolerance and thus enhance the hydrogen yields; however, adaptation and selection of appropriate microbial communities remains uncertain when dealing with complex substrates in a continuous fermentation mode. To address this question, a reactor fed in continuous mode with food waste (organic loading rate of 60 gVS·L·d<sup>−1</sup>; 12 h hydraulic retention time) was first stressed for 48 h with increasing concentrations of butyric acid (up to 8.7 g·L<sup>−1</sup>). Performances were compared with a control reactor (unstressed) for 13 days. During 6 days in a steady-state, the pre-stressed reactor produced 2.2 ± 0.2 LH<sub>2</sub>·L·d<sup>−1</sup>, which was 48% higher than in the control reactor (1.5 ± 0.2 LH<sub>2</sub>·L·d<sup>−1</sup>). The pretreatment also affected the metabolites’ distribution. The pre-stressed reactor presented a higher production of butyric acid (+44%) achieving up to 3.8 ± 0.3 g·L<sup>−1</sup>, a lower production of lactic acid (−56%), and an enhancement of substrate conversion (+9%). The performance improvement was attributed to the promotion of <i>Clostridium guangxiense</i>, a hydrogen -producer, with a relative abundance increasing from 22% in the unstressed reactor to 52% in the stressed reactor. |
| first_indexed | 2024-03-10T01:20:59Z |
| format | Article |
| id | doaj.art-742cb3db803b407aa0e7b75e0c8ab974 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-10T01:20:59Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-742cb3db803b407aa0e7b75e0c8ab9742023-11-23T13:59:27ZengMDPI AGEnergies1996-10732022-05-011511404810.3390/en15114048Enhanced Fermentative Hydrogen Production from Food Waste in Continuous Reactor after Butyric Acid TreatmentMarie Céline Noguer0Jose Antonio Magdalena1Nicolas Bernet2Renaud Escudié3Eric Trably4INRAE, University of Montpellier, LBE, 102 Avenue des Etangs, 11100 Narbonne, FranceINRAE, University of Montpellier, LBE, 102 Avenue des Etangs, 11100 Narbonne, FranceINRAE, University of Montpellier, LBE, 102 Avenue des Etangs, 11100 Narbonne, FranceINRAE, University of Montpellier, LBE, 102 Avenue des Etangs, 11100 Narbonne, FranceINRAE, University of Montpellier, LBE, 102 Avenue des Etangs, 11100 Narbonne, FranceEnd-product accumulation during dark fermentation leads to process instability and hydrogen production inhibition. To overcome this constraint, microbial community adaptation to butyric acid can induce acid tolerance and thus enhance the hydrogen yields; however, adaptation and selection of appropriate microbial communities remains uncertain when dealing with complex substrates in a continuous fermentation mode. To address this question, a reactor fed in continuous mode with food waste (organic loading rate of 60 gVS·L·d<sup>−1</sup>; 12 h hydraulic retention time) was first stressed for 48 h with increasing concentrations of butyric acid (up to 8.7 g·L<sup>−1</sup>). Performances were compared with a control reactor (unstressed) for 13 days. During 6 days in a steady-state, the pre-stressed reactor produced 2.2 ± 0.2 LH<sub>2</sub>·L·d<sup>−1</sup>, which was 48% higher than in the control reactor (1.5 ± 0.2 LH<sub>2</sub>·L·d<sup>−1</sup>). The pretreatment also affected the metabolites’ distribution. The pre-stressed reactor presented a higher production of butyric acid (+44%) achieving up to 3.8 ± 0.3 g·L<sup>−1</sup>, a lower production of lactic acid (−56%), and an enhancement of substrate conversion (+9%). The performance improvement was attributed to the promotion of <i>Clostridium guangxiense</i>, a hydrogen -producer, with a relative abundance increasing from 22% in the unstressed reactor to 52% in the stressed reactor.https://www.mdpi.com/1996-1073/15/11/4048biohydrogenbutyric aciddark fermentationinhibitionmicrobial communitiesstart-up |
| spellingShingle | Marie Céline Noguer Jose Antonio Magdalena Nicolas Bernet Renaud Escudié Eric Trably Enhanced Fermentative Hydrogen Production from Food Waste in Continuous Reactor after Butyric Acid Treatment Energies biohydrogen butyric acid dark fermentation inhibition microbial communities start-up |
| title | Enhanced Fermentative Hydrogen Production from Food Waste in Continuous Reactor after Butyric Acid Treatment |
| title_full | Enhanced Fermentative Hydrogen Production from Food Waste in Continuous Reactor after Butyric Acid Treatment |
| title_fullStr | Enhanced Fermentative Hydrogen Production from Food Waste in Continuous Reactor after Butyric Acid Treatment |
| title_full_unstemmed | Enhanced Fermentative Hydrogen Production from Food Waste in Continuous Reactor after Butyric Acid Treatment |
| title_short | Enhanced Fermentative Hydrogen Production from Food Waste in Continuous Reactor after Butyric Acid Treatment |
| title_sort | enhanced fermentative hydrogen production from food waste in continuous reactor after butyric acid treatment |
| topic | biohydrogen butyric acid dark fermentation inhibition microbial communities start-up |
| url | https://www.mdpi.com/1996-1073/15/11/4048 |
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