Effect of Gaseous Ozone on <i>Listeria monocytogenes</i> Planktonic Cells and Biofilm: An In Vitro Study
Among food-borne pathogens, <i>Listeria monocytogenes</i> continues to pose concerns to food business operators due to its capacity to form biofilm in processing environments. Ozone may be an eco-friendly technology to control microbial contaminations, but data concerning its effect on &...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/7/1484 |
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| author | Felice Panebianco Selene Rubiola Francesco Chiesa Tiziana Civera Pierluigi Aldo Di Ciccio |
| author_facet | Felice Panebianco Selene Rubiola Francesco Chiesa Tiziana Civera Pierluigi Aldo Di Ciccio |
| author_sort | Felice Panebianco |
| collection | DOAJ |
| description | Among food-borne pathogens, <i>Listeria monocytogenes</i> continues to pose concerns to food business operators due to its capacity to form biofilm in processing environments. Ozone may be an eco-friendly technology to control microbial contaminations, but data concerning its effect on <i>Listeria monocytogenes</i> biofilm are still limited. In this study, the effect of gaseous ozone at 50 ppm on planktonic cells and biofilm of reference and food-related <i>Listeria monocytogenes</i> strains was evaluated. Ozone caused a reduction in microbial loads of 3.7 ± 0.4 and 3.9 ± 0.4 Log10 CFU/mL after 10 and 30 min, respectively. A complete inactivation of planktonic cells after 6 h of treatment was observed. Biofilm inhibition and eradication treatments (50 ppm, 6 h) resulted in a significant decrease of the biofilm biomass for 59% of the strains tested, whilst a slight dampening of live cell loads in the biofilm state was observed. In conclusion, gaseous ozone is not sufficient to completely counteract <i>Listeria monocytogenes</i> biofilm, but it may be useful as an additional tool to contrast <i>Listeria monocytogenes</i> free-living cells and to improve the existing sanitization procedures in food processing environments. |
| first_indexed | 2024-03-10T10:01:39Z |
| format | Article |
| id | doaj.art-74315b982ae94ee9bc772ce7eb7d932d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T10:01:39Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-74315b982ae94ee9bc772ce7eb7d932d2023-11-22T01:52:47ZengMDPI AGFoods2304-81582021-06-01107148410.3390/foods10071484Effect of Gaseous Ozone on <i>Listeria monocytogenes</i> Planktonic Cells and Biofilm: An In Vitro StudyFelice Panebianco0Selene Rubiola1Francesco Chiesa2Tiziana Civera3Pierluigi Aldo Di Ciccio4Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyAmong food-borne pathogens, <i>Listeria monocytogenes</i> continues to pose concerns to food business operators due to its capacity to form biofilm in processing environments. Ozone may be an eco-friendly technology to control microbial contaminations, but data concerning its effect on <i>Listeria monocytogenes</i> biofilm are still limited. In this study, the effect of gaseous ozone at 50 ppm on planktonic cells and biofilm of reference and food-related <i>Listeria monocytogenes</i> strains was evaluated. Ozone caused a reduction in microbial loads of 3.7 ± 0.4 and 3.9 ± 0.4 Log10 CFU/mL after 10 and 30 min, respectively. A complete inactivation of planktonic cells after 6 h of treatment was observed. Biofilm inhibition and eradication treatments (50 ppm, 6 h) resulted in a significant decrease of the biofilm biomass for 59% of the strains tested, whilst a slight dampening of live cell loads in the biofilm state was observed. In conclusion, gaseous ozone is not sufficient to completely counteract <i>Listeria monocytogenes</i> biofilm, but it may be useful as an additional tool to contrast <i>Listeria monocytogenes</i> free-living cells and to improve the existing sanitization procedures in food processing environments.https://www.mdpi.com/2304-8158/10/7/1484antimicrobialbiofilm<i>Listeria monocytogenes</i>ozoneeco-friendly technologyfoodborne pathogens |
| spellingShingle | Felice Panebianco Selene Rubiola Francesco Chiesa Tiziana Civera Pierluigi Aldo Di Ciccio Effect of Gaseous Ozone on <i>Listeria monocytogenes</i> Planktonic Cells and Biofilm: An In Vitro Study Foods antimicrobial biofilm <i>Listeria monocytogenes</i> ozone eco-friendly technology foodborne pathogens |
| title | Effect of Gaseous Ozone on <i>Listeria monocytogenes</i> Planktonic Cells and Biofilm: An In Vitro Study |
| title_full | Effect of Gaseous Ozone on <i>Listeria monocytogenes</i> Planktonic Cells and Biofilm: An In Vitro Study |
| title_fullStr | Effect of Gaseous Ozone on <i>Listeria monocytogenes</i> Planktonic Cells and Biofilm: An In Vitro Study |
| title_full_unstemmed | Effect of Gaseous Ozone on <i>Listeria monocytogenes</i> Planktonic Cells and Biofilm: An In Vitro Study |
| title_short | Effect of Gaseous Ozone on <i>Listeria monocytogenes</i> Planktonic Cells and Biofilm: An In Vitro Study |
| title_sort | effect of gaseous ozone on i listeria monocytogenes i planktonic cells and biofilm an in vitro study |
| topic | antimicrobial biofilm <i>Listeria monocytogenes</i> ozone eco-friendly technology foodborne pathogens |
| url | https://www.mdpi.com/2304-8158/10/7/1484 |
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