Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection
Tripartite-motif 21 (TRIM21) is thought to regulate the type I interferon (IFN) response to virus pathogens and serve as a cytosolic Fc receptor for immunoglobulin. Since herpes simplex virus (HSV)-1 is sensitive to type I IFN and neutralizing antibody, we investigated the role of TRIM21 in response...
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MDPI AG
2022-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/3/589 |
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| author | Amanda Berube Grzegorz B. Gmyrek Derek J. Royer Daniel J. J. Carr |
| author_facet | Amanda Berube Grzegorz B. Gmyrek Derek J. Royer Daniel J. J. Carr |
| author_sort | Amanda Berube |
| collection | DOAJ |
| description | Tripartite-motif 21 (TRIM21) is thought to regulate the type I interferon (IFN) response to virus pathogens and serve as a cytosolic Fc receptor for immunoglobulin. Since herpes simplex virus (HSV)-1 is sensitive to type I IFN and neutralizing antibody, we investigated the role of TRIM21 in response to ocular HSV-1 infection in mice. In comparison to wild type (WT) mice, TRIM21 deficient (TRIM21 KO) mice were found to be no more susceptible to ocular HSV-1 infection than WT animals, in terms of infectious virus recovered in the cornea. Similar pathology, in terms of neovascularization, opacity, and loss of peripheral vision function, was observed in both WT and TRIM21 KO mice. However, TRIM21 KO mice did possess a significant increase in infectious virus recovered in the trigeminal ganglia, in comparison to the WT animals. The increased susceptibility was not due to changes in HSV-1-specific CD4+ or CD8+ T cell numbers or functional capabilities, or in changes in type I IFN or IFN-inducible gene expression. In summary, the absence of TRIM21 results in a modest, but significant, increase in HSV-1 titers recovered from the TG of TRIM21 KO mice during acute infection, by a mechanism yet to be determined. |
| first_indexed | 2024-03-09T12:15:52Z |
| format | Article |
| id | doaj.art-7431e9c4f2124a2bbbc05116bc9490cc |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:15:52Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-7431e9c4f2124a2bbbc05116bc9490cc2023-11-30T22:46:45ZengMDPI AGViruses1999-49152022-03-0114358910.3390/v14030589Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea InfectionAmanda Berube0Grzegorz B. Gmyrek1Derek J. Royer2Daniel J. J. Carr3Department of Ophthalmology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USADepartment of Ophthalmology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USADepartment of Biology, East Texas Baptist University, Marshall, TX 75670, USADepartment of Ophthalmology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USATripartite-motif 21 (TRIM21) is thought to regulate the type I interferon (IFN) response to virus pathogens and serve as a cytosolic Fc receptor for immunoglobulin. Since herpes simplex virus (HSV)-1 is sensitive to type I IFN and neutralizing antibody, we investigated the role of TRIM21 in response to ocular HSV-1 infection in mice. In comparison to wild type (WT) mice, TRIM21 deficient (TRIM21 KO) mice were found to be no more susceptible to ocular HSV-1 infection than WT animals, in terms of infectious virus recovered in the cornea. Similar pathology, in terms of neovascularization, opacity, and loss of peripheral vision function, was observed in both WT and TRIM21 KO mice. However, TRIM21 KO mice did possess a significant increase in infectious virus recovered in the trigeminal ganglia, in comparison to the WT animals. The increased susceptibility was not due to changes in HSV-1-specific CD4+ or CD8+ T cell numbers or functional capabilities, or in changes in type I IFN or IFN-inducible gene expression. In summary, the absence of TRIM21 results in a modest, but significant, increase in HSV-1 titers recovered from the TG of TRIM21 KO mice during acute infection, by a mechanism yet to be determined.https://www.mdpi.com/1999-4915/14/3/589HSV-1corneatrigeminal gangliaT cellsinterferon-γinterferon stimulatory genes |
| spellingShingle | Amanda Berube Grzegorz B. Gmyrek Derek J. Royer Daniel J. J. Carr Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection Viruses HSV-1 cornea trigeminal ganglia T cells interferon-γ interferon stimulatory genes |
| title | Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection |
| title_full | Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection |
| title_fullStr | Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection |
| title_full_unstemmed | Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection |
| title_short | Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection |
| title_sort | tripartite motif 21 trim21 deficiency results in a modest loss of herpes simplex virus hsv 1 surveillance in the trigeminal ganglia following cornea infection |
| topic | HSV-1 cornea trigeminal ganglia T cells interferon-γ interferon stimulatory genes |
| url | https://www.mdpi.com/1999-4915/14/3/589 |
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