Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food
Abstract Background Threonine is an essential amino acid that is extensively used in livestock industry as feed supplement due to its pronounced effect in improving the growth performance of animals. Application of genetically engineered bacteria for amino acid production has its share of controvers...
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-019-1173-2 |
| _version_ | 1819207365256282112 |
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| author | Ye Heng Lim Hooi Ling Foo Teck Chwen Loh Rosfarizan Mohamad Raha Abdul Rahim Zulkifli Idrus |
| author_facet | Ye Heng Lim Hooi Ling Foo Teck Chwen Loh Rosfarizan Mohamad Raha Abdul Rahim Zulkifli Idrus |
| author_sort | Ye Heng Lim |
| collection | DOAJ |
| description | Abstract Background Threonine is an essential amino acid that is extensively used in livestock industry as feed supplement due to its pronounced effect in improving the growth performance of animals. Application of genetically engineered bacteria for amino acid production has its share of controversies after eosinophils myalgia syndrome outbreak in 1980s. This has urged for continuous search for a food grade producer as a safer alternative for industrial amino acid production. Lactic acid bacteria (LAB) appear as an exceptional candidate owing to their non-pathogenic nature and reputation of Generally Recognized as Safe (GRAS) status. Recently, we have identified a LAB, Pediococcus pentosaceus TL-3, isolated from Malaysian food as a potential threonine producer. Thus, the objective of this study was to enhance the threonine production by P. pentosaceus TL-3 via optimized medium developed by using Plackett–Burman design (PBD) and central composite design (CCD). Results Molasses, meat extract, (NH4)2SO4, and MnSO4 were identified as the main medium components for threonine production by P. pentosaceus TL-3. The optimum concentration of molasses, meat extract, (NH4)2SO4 and MnSO4 were found to be 30.79 g/L, 25.30 g/L, 8.59 g/L, and 0.098 g/L respectively based on model obtained in CCD with a predicted net threonine production of 123.07 mg/L. The net threonine production by P. pentosaceus TL-3 in the optimized medium was enhanced approximately 2 folds compared to the control. Conclusions This study has revealed the potential of P. pentosaceus TL-3 as a safer alternative to produce threonine. Additionally, the current study has identified the key medium components affecting the production of threonine by P. pentosaceus TL-3, followed by optimization of their concentrations by means of statistical approach. The findings of this study could act as a guideline for the future exploration of amino acid production by LAB. |
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| format | Article |
| id | doaj.art-7431ef657fc144e296cc53a58b817107 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-23T05:22:20Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-7431ef657fc144e296cc53a58b8171072022-12-21T17:58:39ZengBMCMicrobial Cell Factories1475-28592019-07-0118111910.1186/s12934-019-1173-2Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian foodYe Heng Lim0Hooi Ling Foo1Teck Chwen Loh2Rosfarizan Mohamad3Raha Abdul Rahim4Zulkifli Idrus5Institute of Bioscience, Universiti Putra MalaysiaInstitute of Bioscience, Universiti Putra MalaysiaDepartment of Animal Science, Faculty of Agriculture, Universiti Putra MalaysiaDepartment of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaInstitute of Bioscience, Universiti Putra MalaysiaDepartment of Animal Science, Faculty of Agriculture, Universiti Putra MalaysiaAbstract Background Threonine is an essential amino acid that is extensively used in livestock industry as feed supplement due to its pronounced effect in improving the growth performance of animals. Application of genetically engineered bacteria for amino acid production has its share of controversies after eosinophils myalgia syndrome outbreak in 1980s. This has urged for continuous search for a food grade producer as a safer alternative for industrial amino acid production. Lactic acid bacteria (LAB) appear as an exceptional candidate owing to their non-pathogenic nature and reputation of Generally Recognized as Safe (GRAS) status. Recently, we have identified a LAB, Pediococcus pentosaceus TL-3, isolated from Malaysian food as a potential threonine producer. Thus, the objective of this study was to enhance the threonine production by P. pentosaceus TL-3 via optimized medium developed by using Plackett–Burman design (PBD) and central composite design (CCD). Results Molasses, meat extract, (NH4)2SO4, and MnSO4 were identified as the main medium components for threonine production by P. pentosaceus TL-3. The optimum concentration of molasses, meat extract, (NH4)2SO4 and MnSO4 were found to be 30.79 g/L, 25.30 g/L, 8.59 g/L, and 0.098 g/L respectively based on model obtained in CCD with a predicted net threonine production of 123.07 mg/L. The net threonine production by P. pentosaceus TL-3 in the optimized medium was enhanced approximately 2 folds compared to the control. Conclusions This study has revealed the potential of P. pentosaceus TL-3 as a safer alternative to produce threonine. Additionally, the current study has identified the key medium components affecting the production of threonine by P. pentosaceus TL-3, followed by optimization of their concentrations by means of statistical approach. The findings of this study could act as a guideline for the future exploration of amino acid production by LAB.http://link.springer.com/article/10.1186/s12934-019-1173-2Central composite designPediococcus pentosaceus TL-3Plackett–Burman designStatistical optimizationThreonine production |
| spellingShingle | Ye Heng Lim Hooi Ling Foo Teck Chwen Loh Rosfarizan Mohamad Raha Abdul Rahim Zulkifli Idrus Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food Microbial Cell Factories Central composite design Pediococcus pentosaceus TL-3 Plackett–Burman design Statistical optimization Threonine production |
| title | Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food |
| title_full | Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food |
| title_fullStr | Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food |
| title_full_unstemmed | Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food |
| title_short | Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food |
| title_sort | optimized medium via statistical approach enhanced threonine production by pediococcus pentosaceus tl 3 isolated from malaysian food |
| topic | Central composite design Pediococcus pentosaceus TL-3 Plackett–Burman design Statistical optimization Threonine production |
| url | http://link.springer.com/article/10.1186/s12934-019-1173-2 |
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