Proteinuria increases oxylipid concentrations in VLDL and HDL but not LDL particles in the rat
We previously established that proteinuria alters the apolipoprotein content of lipoproteins. This study was conducted to establish whether proteinuria also alters the concentrations of oxidized lipids within lipoprotein density fractions. To this end, we induced passive Heymann nephritis in Sprague...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2007-08-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520425155 |
| _version_ | 1818972482655223808 |
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| author | John W. Newman George A. Kaysen Bruce D. Hammock Gregory C. Shearer |
| author_facet | John W. Newman George A. Kaysen Bruce D. Hammock Gregory C. Shearer |
| author_sort | John W. Newman |
| collection | DOAJ |
| description | We previously established that proteinuria alters the apolipoprotein content of lipoproteins. This study was conducted to establish whether proteinuria also alters the concentrations of oxidized lipids within lipoprotein density fractions. To this end, we induced passive Heymann nephritis in Sprague Dawley rats and measured an array of alkaline-stable oxylipids in VLDL, LDL, and HDL particles. Proteinuria increased the total oxylipid amounts in the HDL and VLDL fractions. More importantly, these levels were increased when expressed per unit lipoprotein protein, indicating that the oxidized lipid load per particle was increased. Epoxides and diols increased ∼2-fold in HDL and ∼5-fold in VLDL, whereas LDL showed ∼2-fold decreases. The hydroxyeicosatetraenoic acids and hydroxyoctadecadienoic acids (HODEs) increased >4-fold in HDL and >20-fold in VLDL, whereas LDL showed ∼2-fold decreases in the HODEs. Therefore, nephrotic syndrome alters the lipoprotein oxylipid composition independently of an increase in total lipoprotein levels. These proteinuria-induced changes may be associated with the cardiovascular risk of lipoprotein oxidation. |
| first_indexed | 2024-12-20T15:08:58Z |
| format | Article |
| id | doaj.art-7433702086664245929b8371f62f6b97 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T15:08:58Z |
| publishDate | 2007-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7433702086664245929b8371f62f6b972022-12-21T19:36:23ZengElsevierJournal of Lipid Research0022-22752007-08-0148817921800Proteinuria increases oxylipid concentrations in VLDL and HDL but not LDL particles in the ratJohn W. Newman0George A. Kaysen1Bruce D. Hammock2Gregory C. Shearer3Western Human Nutrition Research Center, United States Department of Agriculture, Davis, CA; Department of Nutrition, University of California Davis, Davis, CA; Department of Entomology, University of California Davis, Davis, CADepartment of Internal Medicine, University of California Davis, Davis, CA; Department of Veterans Affairs, Northern California Health Care System, Mather, CADepartment of Entomology, University of California Davis, Davis, CA; UC Davis Cancer Center, University of California Davis, Davis, CADepartment of Internal Medicine, University of California Davis, Davis, CA; Department of Veterans Affairs, Northern California Health Care System, Mather, CAWe previously established that proteinuria alters the apolipoprotein content of lipoproteins. This study was conducted to establish whether proteinuria also alters the concentrations of oxidized lipids within lipoprotein density fractions. To this end, we induced passive Heymann nephritis in Sprague Dawley rats and measured an array of alkaline-stable oxylipids in VLDL, LDL, and HDL particles. Proteinuria increased the total oxylipid amounts in the HDL and VLDL fractions. More importantly, these levels were increased when expressed per unit lipoprotein protein, indicating that the oxidized lipid load per particle was increased. Epoxides and diols increased ∼2-fold in HDL and ∼5-fold in VLDL, whereas LDL showed ∼2-fold decreases. The hydroxyeicosatetraenoic acids and hydroxyoctadecadienoic acids (HODEs) increased >4-fold in HDL and >20-fold in VLDL, whereas LDL showed ∼2-fold decreases in the HODEs. Therefore, nephrotic syndrome alters the lipoprotein oxylipid composition independently of an increase in total lipoprotein levels. These proteinuria-induced changes may be associated with the cardiovascular risk of lipoprotein oxidation.http://www.sciencedirect.com/science/article/pii/S0022227520425155hyperlipidemiametabolic profilingeicosanoidsoctadecanoidsnephrotic syndromeoxylipin |
| spellingShingle | John W. Newman George A. Kaysen Bruce D. Hammock Gregory C. Shearer Proteinuria increases oxylipid concentrations in VLDL and HDL but not LDL particles in the rat Journal of Lipid Research hyperlipidemia metabolic profiling eicosanoids octadecanoids nephrotic syndrome oxylipin |
| title | Proteinuria increases oxylipid concentrations in VLDL and HDL but not LDL particles in the rat |
| title_full | Proteinuria increases oxylipid concentrations in VLDL and HDL but not LDL particles in the rat |
| title_fullStr | Proteinuria increases oxylipid concentrations in VLDL and HDL but not LDL particles in the rat |
| title_full_unstemmed | Proteinuria increases oxylipid concentrations in VLDL and HDL but not LDL particles in the rat |
| title_short | Proteinuria increases oxylipid concentrations in VLDL and HDL but not LDL particles in the rat |
| title_sort | proteinuria increases oxylipid concentrations in vldl and hdl but not ldl particles in the rat |
| topic | hyperlipidemia metabolic profiling eicosanoids octadecanoids nephrotic syndrome oxylipin |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520425155 |
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