Insight into the Modification of Phosphatidylcholine with n-3 Polyunsaturated Fatty Acids-Rich Ethyl Esters by Immobilized MAS1 Lipase
This study reported the modification of phosphatidylcholine (PC) with n-3 polyunsaturated fatty acids (PUFA)-rich ethyl esters (EE) by immobilized MAS1 lipase-catalyzed transesterification in the solvent-free system. Effects of n-3 PUFA-rich EE/PC mass ratio, enzyme loading, reaction temperature, an...
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| Format: | Article |
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MDPI AG
2019-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/19/3528 |
| _version_ | 1818411990406660096 |
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| author | Xiumei Wang Xiaoli Qin Xiuting Li Zexin Zhao Bo Yang Yonghua Wang |
| author_facet | Xiumei Wang Xiaoli Qin Xiuting Li Zexin Zhao Bo Yang Yonghua Wang |
| author_sort | Xiumei Wang |
| collection | DOAJ |
| description | This study reported the modification of phosphatidylcholine (PC) with n-3 polyunsaturated fatty acids (PUFA)-rich ethyl esters (EE) by immobilized MAS1 lipase-catalyzed transesterification in the solvent-free system. Effects of n-3 PUFA-rich EE/PC mass ratio, enzyme loading, reaction temperature, and water dosage on the incorporation of n-3 PUFA into PC were investigated, respectively. The results indicate that the maximum incorporation of n-3 PUFA into PC reached 33.5% (24 h) under the following conditions: n-3 PUFA-rich EE/PC mass ratio of 6:1, enzyme loading of 20%, reaction temperature of 55 °C, and water dosage of 1.0%. After 72 h of reaction, the incorporation of n-3 PUFA into PC was 43.55% and the composition of the reaction mixture was analyzed by <sup>31</sup>P nuclear magnetic resonance (NMR). The results show that the reaction product consisted of 32.68% PC, 28.76% 1-diacyl-<i>sn</i>-glycero-3-lysophosphatidylcholine (<i>sn</i>-1 LPC), 4.90% 2-diacyl-<i>sn</i>-glycero-3-lysophosphatidylcholine (<i>sn</i>-2 LPC), and 33.60% <i>sn</i>-glycero-3-phosphatidylcholine (GPC). This study offers insight into the phospholipase activity of immobilized MAS1 lipase and suggests the extended applications of immobilized MAS1 lipase in the modification of phospholipids for industrial purpose. |
| first_indexed | 2024-12-14T10:40:11Z |
| format | Article |
| id | doaj.art-743a6d320fec4d1aaa42894630f2017e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-14T10:40:11Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-743a6d320fec4d1aaa42894630f2017e2022-12-21T23:05:43ZengMDPI AGMolecules1420-30492019-09-012419352810.3390/molecules24193528molecules24193528Insight into the Modification of Phosphatidylcholine with n-3 Polyunsaturated Fatty Acids-Rich Ethyl Esters by Immobilized MAS1 LipaseXiumei Wang0Xiaoli Qin1Xiuting Li2Zexin Zhao3Bo Yang4Yonghua Wang5Key Laboratory of Loquat Germplasm Innovation and Utilization, Fujian Province University, College of Environmental and Biological Engineering, Putian University, Putian 351100, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, ChinaSchool of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, ChinaGuangdong Research Center of Lipid Science and Applied Engineering Technology, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaThis study reported the modification of phosphatidylcholine (PC) with n-3 polyunsaturated fatty acids (PUFA)-rich ethyl esters (EE) by immobilized MAS1 lipase-catalyzed transesterification in the solvent-free system. Effects of n-3 PUFA-rich EE/PC mass ratio, enzyme loading, reaction temperature, and water dosage on the incorporation of n-3 PUFA into PC were investigated, respectively. The results indicate that the maximum incorporation of n-3 PUFA into PC reached 33.5% (24 h) under the following conditions: n-3 PUFA-rich EE/PC mass ratio of 6:1, enzyme loading of 20%, reaction temperature of 55 °C, and water dosage of 1.0%. After 72 h of reaction, the incorporation of n-3 PUFA into PC was 43.55% and the composition of the reaction mixture was analyzed by <sup>31</sup>P nuclear magnetic resonance (NMR). The results show that the reaction product consisted of 32.68% PC, 28.76% 1-diacyl-<i>sn</i>-glycero-3-lysophosphatidylcholine (<i>sn</i>-1 LPC), 4.90% 2-diacyl-<i>sn</i>-glycero-3-lysophosphatidylcholine (<i>sn</i>-2 LPC), and 33.60% <i>sn</i>-glycero-3-phosphatidylcholine (GPC). This study offers insight into the phospholipase activity of immobilized MAS1 lipase and suggests the extended applications of immobilized MAS1 lipase in the modification of phospholipids for industrial purpose.https://www.mdpi.com/1420-3049/24/19/3528immobilized mas1 lipasephosphatidylcholinen-3 polyunsaturated fatty acidsethyl esterstransesterification |
| spellingShingle | Xiumei Wang Xiaoli Qin Xiuting Li Zexin Zhao Bo Yang Yonghua Wang Insight into the Modification of Phosphatidylcholine with n-3 Polyunsaturated Fatty Acids-Rich Ethyl Esters by Immobilized MAS1 Lipase Molecules immobilized mas1 lipase phosphatidylcholine n-3 polyunsaturated fatty acids ethyl esters transesterification |
| title | Insight into the Modification of Phosphatidylcholine with n-3 Polyunsaturated Fatty Acids-Rich Ethyl Esters by Immobilized MAS1 Lipase |
| title_full | Insight into the Modification of Phosphatidylcholine with n-3 Polyunsaturated Fatty Acids-Rich Ethyl Esters by Immobilized MAS1 Lipase |
| title_fullStr | Insight into the Modification of Phosphatidylcholine with n-3 Polyunsaturated Fatty Acids-Rich Ethyl Esters by Immobilized MAS1 Lipase |
| title_full_unstemmed | Insight into the Modification of Phosphatidylcholine with n-3 Polyunsaturated Fatty Acids-Rich Ethyl Esters by Immobilized MAS1 Lipase |
| title_short | Insight into the Modification of Phosphatidylcholine with n-3 Polyunsaturated Fatty Acids-Rich Ethyl Esters by Immobilized MAS1 Lipase |
| title_sort | insight into the modification of phosphatidylcholine with n 3 polyunsaturated fatty acids rich ethyl esters by immobilized mas1 lipase |
| topic | immobilized mas1 lipase phosphatidylcholine n-3 polyunsaturated fatty acids ethyl esters transesterification |
| url | https://www.mdpi.com/1420-3049/24/19/3528 |
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