Hepatoprotective effects of astragalin against polystyrene microplastics induced hepatic damage in male albino rats by modulating Nrf-2/Keap-1 pathway
The current study was designed to determine the curative potential of astragalin (AST) against polystyrene microplastics (PS-MPs) induced hepatic toxicity in rats. PS-MPs exposure decreased the expression of Nrf-2 and anti-oxidant enzymes, while increasing Keap-1 expression. The activities of glutat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623003717 |
| _version_ | 1797688969011396608 |
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| author | Ali Hamza Muhammad Umar Ijaz Nazia Ehsan Hammad Ahmad Khan Saad Alkahtani Usman Atique |
| author_facet | Ali Hamza Muhammad Umar Ijaz Nazia Ehsan Hammad Ahmad Khan Saad Alkahtani Usman Atique |
| author_sort | Ali Hamza |
| collection | DOAJ |
| description | The current study was designed to determine the curative potential of astragalin (AST) against polystyrene microplastics (PS-MPs) induced hepatic toxicity in rats. PS-MPs exposure decreased the expression of Nrf-2 and anti-oxidant enzymes, while increasing Keap-1 expression. The activities of glutathione-S-transferase, catalase, glutathione, glutathione peroxidase, superoxide dismutase, glutathione reductase and heme oxygenase-1 were decreased, besides the levels of malondialdehyde and reactive oxygen species were also increased following the exposure of PS-MPs. The intoxication of PS-MPs elevated aspartate aminotransferase, alanine transaminase, alkaline phosphatase levels, as well as cyclooxygenase-2 activity, interleukin-6, interleukin-1 beta, nuclear factor-kappa B and tumor necrosis factor-alpha levels were also escalated. Furthermore, Bcl-2 expression was down-regulated, while Bax and Caspase-3 expressions were upregulated following PS-MPs exposure. Histopathological assessment revealed substantial liver damages in PS-MPs treated rats. However, AST supplementation substantially recovered PS-MPs-induced damages and histological anomalies. Therefore, AST can be used as a curative agent to treat PS-MPs-prompted hepatotoxicity due to its anti-apoptotic, anti-inflammatory and anti-oxidant potentials. |
| first_indexed | 2024-03-12T01:38:13Z |
| format | Article |
| id | doaj.art-743b26a15168471ba9bbeec6e64e1356 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-12T01:38:13Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-743b26a15168471ba9bbeec6e64e13562023-09-11T04:16:57ZengElsevierJournal of Functional Foods1756-46462023-09-01108105771Hepatoprotective effects of astragalin against polystyrene microplastics induced hepatic damage in male albino rats by modulating Nrf-2/Keap-1 pathwayAli Hamza0Muhammad Umar Ijaz1Nazia Ehsan2Hammad Ahmad Khan3Saad Alkahtani4Usman Atique5Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad 38040, PakistanDepartment of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad 38040, Pakistan; Corresponding author.Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad 38040, PakistanDepartment of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad 38040, PakistanDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaCollege of Biological Systems, Chungnam National University, Daejeon 34134, South KoreaThe current study was designed to determine the curative potential of astragalin (AST) against polystyrene microplastics (PS-MPs) induced hepatic toxicity in rats. PS-MPs exposure decreased the expression of Nrf-2 and anti-oxidant enzymes, while increasing Keap-1 expression. The activities of glutathione-S-transferase, catalase, glutathione, glutathione peroxidase, superoxide dismutase, glutathione reductase and heme oxygenase-1 were decreased, besides the levels of malondialdehyde and reactive oxygen species were also increased following the exposure of PS-MPs. The intoxication of PS-MPs elevated aspartate aminotransferase, alanine transaminase, alkaline phosphatase levels, as well as cyclooxygenase-2 activity, interleukin-6, interleukin-1 beta, nuclear factor-kappa B and tumor necrosis factor-alpha levels were also escalated. Furthermore, Bcl-2 expression was down-regulated, while Bax and Caspase-3 expressions were upregulated following PS-MPs exposure. Histopathological assessment revealed substantial liver damages in PS-MPs treated rats. However, AST supplementation substantially recovered PS-MPs-induced damages and histological anomalies. Therefore, AST can be used as a curative agent to treat PS-MPs-prompted hepatotoxicity due to its anti-apoptotic, anti-inflammatory and anti-oxidant potentials.http://www.sciencedirect.com/science/article/pii/S1756464623003717Polystyrene microplasticsAstragalinHepatotoxicityInflammationApoptosis |
| spellingShingle | Ali Hamza Muhammad Umar Ijaz Nazia Ehsan Hammad Ahmad Khan Saad Alkahtani Usman Atique Hepatoprotective effects of astragalin against polystyrene microplastics induced hepatic damage in male albino rats by modulating Nrf-2/Keap-1 pathway Journal of Functional Foods Polystyrene microplastics Astragalin Hepatotoxicity Inflammation Apoptosis |
| title | Hepatoprotective effects of astragalin against polystyrene microplastics induced hepatic damage in male albino rats by modulating Nrf-2/Keap-1 pathway |
| title_full | Hepatoprotective effects of astragalin against polystyrene microplastics induced hepatic damage in male albino rats by modulating Nrf-2/Keap-1 pathway |
| title_fullStr | Hepatoprotective effects of astragalin against polystyrene microplastics induced hepatic damage in male albino rats by modulating Nrf-2/Keap-1 pathway |
| title_full_unstemmed | Hepatoprotective effects of astragalin against polystyrene microplastics induced hepatic damage in male albino rats by modulating Nrf-2/Keap-1 pathway |
| title_short | Hepatoprotective effects of astragalin against polystyrene microplastics induced hepatic damage in male albino rats by modulating Nrf-2/Keap-1 pathway |
| title_sort | hepatoprotective effects of astragalin against polystyrene microplastics induced hepatic damage in male albino rats by modulating nrf 2 keap 1 pathway |
| topic | Polystyrene microplastics Astragalin Hepatotoxicity Inflammation Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623003717 |
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