Green Synthesized Silver Nanoparticle-Loaded Liposome-Based Nanoarchitectonics for Cancer Management: In Vitro Drug Release Analysis
Silver nanoparticles act as antitumor agents because of their antiproliferative and apoptosis-inducing properties. The present study aims to develop silver nanoparticle-loaded liposomes for the effective management of cancer. Silver nanoparticle-encapsulated liposomes were prepared using the thin-fi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/11/1/217 |
| _version_ | 1797445603666427904 |
|---|---|
| author | Priyanka Jayachandran Suganya Ilango Vivekananthan Suseela Ramalingam Nirmaladevi Mohammed Rafi Shaik Mujeeb Khan Merajuddin Khan Baji Shaik |
| author_facet | Priyanka Jayachandran Suganya Ilango Vivekananthan Suseela Ramalingam Nirmaladevi Mohammed Rafi Shaik Mujeeb Khan Merajuddin Khan Baji Shaik |
| author_sort | Priyanka Jayachandran |
| collection | DOAJ |
| description | Silver nanoparticles act as antitumor agents because of their antiproliferative and apoptosis-inducing properties. The present study aims to develop silver nanoparticle-loaded liposomes for the effective management of cancer. Silver nanoparticle-encapsulated liposomes were prepared using the thin-film hydration method coupled with sonication. The prepared liposomes were characterized by DLS (Dynamic Light Scattering analysis), FESEM (Field Emission Scanning Electron Microscope), and FTIR (Fourier Transform Infrared spectroscopy). The in vitro drug release profile of the silver nanoparticle-loaded liposomes was carried out using the dialysis bag method and the drug release profile was validated using various mathematical models. <b>A</b> high encapsulation efficiency of silver nanoparticle-loaded liposome was observed (82.25%). A particle size and polydispersity index of 172.1 nm and 0.381, respectively, and the zeta potential of −21.5 mV were recorded. FESEM analysis revealed spherical-shaped nanoparticles in the size range of 80–97 nm. The in vitro drug release profile of the silver nanoparticle-loaded liposomes was carried out using the dialysis bag method in three different pHs: pH 5.5, pH 6.8, and pH 7.4. A high silver nanoparticle release was observed in pH 5.5 which corresponds to the mature endosomes of tumor cells; 73.32 ± 0.68% nanoparticle was released at 72 h in pH 5.5. Among the various mathematical models analyzed, the Higuchi model was the best-fitted model as there is the highest value of the correlation coefficient which confirms that the drug release follows the diffusion-controlled process. From the Korsmeyer–Peppas model, it was confirmed that the drug release is based on anomalous non-Fickian diffusion. The results indicate that the silver nanoparticle-loaded liposomes can be used as an efficient drug delivery carrier to target cancer cells of various types. |
| first_indexed | 2024-03-09T13:28:15Z |
| format | Article |
| id | doaj.art-743bad9ccadd4caa9b0c33173c4e67db |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T13:28:15Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-743bad9ccadd4caa9b0c33173c4e67db2023-11-30T21:21:11ZengMDPI AGBiomedicines2227-90592023-01-0111121710.3390/biomedicines11010217Green Synthesized Silver Nanoparticle-Loaded Liposome-Based Nanoarchitectonics for Cancer Management: In Vitro Drug Release AnalysisPriyanka Jayachandran0Suganya Ilango1Vivekananthan Suseela2Ramalingam Nirmaladevi3Mohammed Rafi Shaik4Mujeeb Khan5Merajuddin Khan6Baji Shaik7Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore 641043, Tamilnadu, IndiaDepartment of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore 641043, Tamilnadu, IndiaDepartment of Biochemistry, P.S.G College of Arts and Science, Coimbatore 641014, Tamilnadu, IndiaDepartment of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore 641043, Tamilnadu, IndiaDepartment of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaSchool of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Republic of KoreaSilver nanoparticles act as antitumor agents because of their antiproliferative and apoptosis-inducing properties. The present study aims to develop silver nanoparticle-loaded liposomes for the effective management of cancer. Silver nanoparticle-encapsulated liposomes were prepared using the thin-film hydration method coupled with sonication. The prepared liposomes were characterized by DLS (Dynamic Light Scattering analysis), FESEM (Field Emission Scanning Electron Microscope), and FTIR (Fourier Transform Infrared spectroscopy). The in vitro drug release profile of the silver nanoparticle-loaded liposomes was carried out using the dialysis bag method and the drug release profile was validated using various mathematical models. <b>A</b> high encapsulation efficiency of silver nanoparticle-loaded liposome was observed (82.25%). A particle size and polydispersity index of 172.1 nm and 0.381, respectively, and the zeta potential of −21.5 mV were recorded. FESEM analysis revealed spherical-shaped nanoparticles in the size range of 80–97 nm. The in vitro drug release profile of the silver nanoparticle-loaded liposomes was carried out using the dialysis bag method in three different pHs: pH 5.5, pH 6.8, and pH 7.4. A high silver nanoparticle release was observed in pH 5.5 which corresponds to the mature endosomes of tumor cells; 73.32 ± 0.68% nanoparticle was released at 72 h in pH 5.5. Among the various mathematical models analyzed, the Higuchi model was the best-fitted model as there is the highest value of the correlation coefficient which confirms that the drug release follows the diffusion-controlled process. From the Korsmeyer–Peppas model, it was confirmed that the drug release is based on anomalous non-Fickian diffusion. The results indicate that the silver nanoparticle-loaded liposomes can be used as an efficient drug delivery carrier to target cancer cells of various types.https://www.mdpi.com/2227-9059/11/1/217silver nanoparticlesliposomesdynamic light scatteringdrug releasemathematical modelsrelease kinetics |
| spellingShingle | Priyanka Jayachandran Suganya Ilango Vivekananthan Suseela Ramalingam Nirmaladevi Mohammed Rafi Shaik Mujeeb Khan Merajuddin Khan Baji Shaik Green Synthesized Silver Nanoparticle-Loaded Liposome-Based Nanoarchitectonics for Cancer Management: In Vitro Drug Release Analysis Biomedicines silver nanoparticles liposomes dynamic light scattering drug release mathematical models release kinetics |
| title | Green Synthesized Silver Nanoparticle-Loaded Liposome-Based Nanoarchitectonics for Cancer Management: In Vitro Drug Release Analysis |
| title_full | Green Synthesized Silver Nanoparticle-Loaded Liposome-Based Nanoarchitectonics for Cancer Management: In Vitro Drug Release Analysis |
| title_fullStr | Green Synthesized Silver Nanoparticle-Loaded Liposome-Based Nanoarchitectonics for Cancer Management: In Vitro Drug Release Analysis |
| title_full_unstemmed | Green Synthesized Silver Nanoparticle-Loaded Liposome-Based Nanoarchitectonics for Cancer Management: In Vitro Drug Release Analysis |
| title_short | Green Synthesized Silver Nanoparticle-Loaded Liposome-Based Nanoarchitectonics for Cancer Management: In Vitro Drug Release Analysis |
| title_sort | green synthesized silver nanoparticle loaded liposome based nanoarchitectonics for cancer management in vitro drug release analysis |
| topic | silver nanoparticles liposomes dynamic light scattering drug release mathematical models release kinetics |
| url | https://www.mdpi.com/2227-9059/11/1/217 |
| work_keys_str_mv | AT priyankajayachandran greensynthesizedsilvernanoparticleloadedliposomebasednanoarchitectonicsforcancermanagementinvitrodrugreleaseanalysis AT suganyailango greensynthesizedsilvernanoparticleloadedliposomebasednanoarchitectonicsforcancermanagementinvitrodrugreleaseanalysis AT vivekananthansuseela greensynthesizedsilvernanoparticleloadedliposomebasednanoarchitectonicsforcancermanagementinvitrodrugreleaseanalysis AT ramalingamnirmaladevi greensynthesizedsilvernanoparticleloadedliposomebasednanoarchitectonicsforcancermanagementinvitrodrugreleaseanalysis AT mohammedrafishaik greensynthesizedsilvernanoparticleloadedliposomebasednanoarchitectonicsforcancermanagementinvitrodrugreleaseanalysis AT mujeebkhan greensynthesizedsilvernanoparticleloadedliposomebasednanoarchitectonicsforcancermanagementinvitrodrugreleaseanalysis AT merajuddinkhan greensynthesizedsilvernanoparticleloadedliposomebasednanoarchitectonicsforcancermanagementinvitrodrugreleaseanalysis AT bajishaik greensynthesizedsilvernanoparticleloadedliposomebasednanoarchitectonicsforcancermanagementinvitrodrugreleaseanalysis |