Production of a Fermented Solid Containing Lipases of Rhizopus microsporus and Its Application in the Pre-Hydrolysis of a High-Fat Dairy Wastewater
The filamentous fungus Rhizopus microsporus CPQBA 312-07 DRM was grown in solid-state cultivation and the fermented solid produced was used to hydrolyze triacylglycerols in a high-fat dairy wastewater. For the solid-state cultivation, a mixture of sunflower seed meal and sugarcane bagasse (1:3 by ma...
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| Format: | Article |
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University of Zagreb Faculty of Food Technology and Biotechnology
2010-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/74729 |
| _version_ | 1797425060705730560 |
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| author | Dayane Alberton David Alexander Mitchell Jesús Cordova Patrício Peralta-Zamora Nadia Krieger |
| author_facet | Dayane Alberton David Alexander Mitchell Jesús Cordova Patrício Peralta-Zamora Nadia Krieger |
| author_sort | Dayane Alberton |
| collection | DOAJ |
| description | The filamentous fungus Rhizopus microsporus CPQBA 312-07 DRM was grown in solid-state cultivation and the fermented solid produced was used to hydrolyze triacylglycerols in a high-fat dairy wastewater. For the solid-state cultivation, a mixture of sunflower seed meal and sugarcane bagasse (1:3 by mass on dry basis) was selected. After 18 h of culture, the fermented product had an activity, measured titrimetrically against triolein, of 26 U per gram of dry solids. This substrate mixture does not suffer from compaction and therefore can be used in large scale solid-state cultivation bioreactors. When used to pretreat a high-fat dairy wastewater, with an oil and grease level above 1300 mg/L, the fermented solid reduced the oil and grease level to below 300 mg/L after 72 h at 35 °C. Further work is required to improve the production of lipolytic activity in the solid-state cultivation step and to find the optimum pretreatment time in the wastewater pretreatment step. |
| first_indexed | 2024-03-09T08:10:36Z |
| format | Article |
| id | doaj.art-7440ae24d497429da69a036f7a4dfb9a |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T08:10:36Z |
| publishDate | 2010-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-7440ae24d497429da69a036f7a4dfb9a2023-12-02T23:25:40ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062010-01-014812835Production of a Fermented Solid Containing Lipases of Rhizopus microsporus and Its Application in the Pre-Hydrolysis of a High-Fat Dairy WastewaterDayane Alberton0David Alexander Mitchell1Jesús Cordova2Patrício Peralta-Zamora3Nadia Krieger4Department of Biochemistry and Molecular Biology, Federal University of Paraná, Centro Politécnico, P.O. Box 19046, Curitiba 81531-980, Paraná, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Paraná, Centro Politécnico, P.O. Box 19046, Curitiba 81531-980, Paraná, BrazilDepartment of Chemical Engineering, CUCEI, University of Guadalajara, Blvd. Marcelino García Barragán y Calz. Olímpica, 44840Department of Chemistry, Federal University of Paraná, Centro Politécnico, P.O. Box 19081, Curitiba 81531-980, Paraná, BrazilDepartment of Chemistry, Federal University of Paraná, Centro Politécnico, P.O. Box 19081, Curitiba 81531-980, Paraná, BrazilThe filamentous fungus Rhizopus microsporus CPQBA 312-07 DRM was grown in solid-state cultivation and the fermented solid produced was used to hydrolyze triacylglycerols in a high-fat dairy wastewater. For the solid-state cultivation, a mixture of sunflower seed meal and sugarcane bagasse (1:3 by mass on dry basis) was selected. After 18 h of culture, the fermented product had an activity, measured titrimetrically against triolein, of 26 U per gram of dry solids. This substrate mixture does not suffer from compaction and therefore can be used in large scale solid-state cultivation bioreactors. When used to pretreat a high-fat dairy wastewater, with an oil and grease level above 1300 mg/L, the fermented solid reduced the oil and grease level to below 300 mg/L after 72 h at 35 °C. Further work is required to improve the production of lipolytic activity in the solid-state cultivation step and to find the optimum pretreatment time in the wastewater pretreatment step.http://hrcak.srce.hr/file/74729lipolysisenzymatic pretreatmentsolid-state fermentationsolid-state cultivationdairy wastewaterRhizopus microsporus |
| spellingShingle | Dayane Alberton David Alexander Mitchell Jesús Cordova Patrício Peralta-Zamora Nadia Krieger Production of a Fermented Solid Containing Lipases of Rhizopus microsporus and Its Application in the Pre-Hydrolysis of a High-Fat Dairy Wastewater Food Technology and Biotechnology lipolysis enzymatic pretreatment solid-state fermentation solid-state cultivation dairy wastewater Rhizopus microsporus |
| title | Production of a Fermented Solid Containing Lipases of Rhizopus microsporus and Its Application in the Pre-Hydrolysis of a High-Fat Dairy Wastewater |
| title_full | Production of a Fermented Solid Containing Lipases of Rhizopus microsporus and Its Application in the Pre-Hydrolysis of a High-Fat Dairy Wastewater |
| title_fullStr | Production of a Fermented Solid Containing Lipases of Rhizopus microsporus and Its Application in the Pre-Hydrolysis of a High-Fat Dairy Wastewater |
| title_full_unstemmed | Production of a Fermented Solid Containing Lipases of Rhizopus microsporus and Its Application in the Pre-Hydrolysis of a High-Fat Dairy Wastewater |
| title_short | Production of a Fermented Solid Containing Lipases of Rhizopus microsporus and Its Application in the Pre-Hydrolysis of a High-Fat Dairy Wastewater |
| title_sort | production of a fermented solid containing lipases of rhizopus microsporus and its application in the pre hydrolysis of a high fat dairy wastewater |
| topic | lipolysis enzymatic pretreatment solid-state fermentation solid-state cultivation dairy wastewater Rhizopus microsporus |
| url | http://hrcak.srce.hr/file/74729 |
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