Conjugation of vascular endothelial growth factor to poly lactic-co-glycolic acid nanospheres enhances differentiation of embryonic stem cells to lymphatic endothelial cells
Objective Pluripotent stem cell-derived lymphatic endothelial cells (LECs) show great promise in their therapeutic application in the field of regenerative medicine related to lymphatic vessels. We tested the approach of forced differentiation of mouse embryonal stem cells into LECs using biodegrada...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2021-04-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ajas-20-0202.pdf |
| _version_ | 1818911896502272000 |
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| author | Hyunjin Yoo Dongyoon Choi Youngsok Choi |
| author_facet | Hyunjin Yoo Dongyoon Choi Youngsok Choi |
| author_sort | Hyunjin Yoo |
| collection | DOAJ |
| description | Objective Pluripotent stem cell-derived lymphatic endothelial cells (LECs) show great promise in their therapeutic application in the field of regenerative medicine related to lymphatic vessels. We tested the approach of forced differentiation of mouse embryonal stem cells into LECs using biodegradable poly lactic-co-glycolic acid (PLGA) nanospheres in conjugation with growth factors (vascular endothelial growth factors [VEGF-A and VEGF-C]). Methods We evaluated the practical use of heparin-conjugated PLGA nanoparticles (molecular weight ~15,000) in conjugation with VEGF-A/C, embryoid body (EB) formation, and LEC differentiation using immunofluorescence staining followed by quantification and quantitative real-time polymerase chain reaction analysis. Results We showed that formation and differentiation of EB with VEGF-A/C-conjugated PLGA nanospheres, compared to direct supplementation of VEGF-A/C to the EB differentiation media, greatly improved yield of LYVE1(+) LECs. Our analyses revealed that the enhanced potential of LEC differentiation using VEGF-A/C-conjugated PLGA nanospheres was mediated by elevation of expression of the genes that are important for lymphatic vessel formation. Conclusion Together, we not only established an improved protocol for LEC differentiation using PLGA nanospheres but also provided a platform technology for the mechanistic study of LEC development in mammals. |
| first_indexed | 2024-12-19T23:05:59Z |
| format | Article |
| id | doaj.art-7446010ec5f248d9b1b898fdca9a3d16 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-12-19T23:05:59Z |
| publishDate | 2021-04-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-7446010ec5f248d9b1b898fdca9a3d162022-12-21T20:02:22ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352021-04-0134453353810.5713/ajas.20.020224566Conjugation of vascular endothelial growth factor to poly lactic-co-glycolic acid nanospheres enhances differentiation of embryonic stem cells to lymphatic endothelial cellsHyunjin Yoo0Dongyoon Choi1Youngsok Choi2 Department of Stem Cell and Regenerative Biotechnology, Institute of Advanced and Regenerative Science, Konkuk University, Seoul 05029, Korea Department of Stem Cell and Regenerative Biotechnology, Institute of Advanced and Regenerative Science, Konkuk University, Seoul 05029, Korea Department of Stem Cell and Regenerative Biotechnology, Institute of Advanced and Regenerative Science, Konkuk University, Seoul 05029, KoreaObjective Pluripotent stem cell-derived lymphatic endothelial cells (LECs) show great promise in their therapeutic application in the field of regenerative medicine related to lymphatic vessels. We tested the approach of forced differentiation of mouse embryonal stem cells into LECs using biodegradable poly lactic-co-glycolic acid (PLGA) nanospheres in conjugation with growth factors (vascular endothelial growth factors [VEGF-A and VEGF-C]). Methods We evaluated the practical use of heparin-conjugated PLGA nanoparticles (molecular weight ~15,000) in conjugation with VEGF-A/C, embryoid body (EB) formation, and LEC differentiation using immunofluorescence staining followed by quantification and quantitative real-time polymerase chain reaction analysis. Results We showed that formation and differentiation of EB with VEGF-A/C-conjugated PLGA nanospheres, compared to direct supplementation of VEGF-A/C to the EB differentiation media, greatly improved yield of LYVE1(+) LECs. Our analyses revealed that the enhanced potential of LEC differentiation using VEGF-A/C-conjugated PLGA nanospheres was mediated by elevation of expression of the genes that are important for lymphatic vessel formation. Conclusion Together, we not only established an improved protocol for LEC differentiation using PLGA nanospheres but also provided a platform technology for the mechanistic study of LEC development in mammals.http://www.animbiosci.org/upload/pdf/ajas-20-0202.pdfstem celllymphatic endothelial cellpoly lactic-co-glycolic acid (plga) |
| spellingShingle | Hyunjin Yoo Dongyoon Choi Youngsok Choi Conjugation of vascular endothelial growth factor to poly lactic-co-glycolic acid nanospheres enhances differentiation of embryonic stem cells to lymphatic endothelial cells Animal Bioscience stem cell lymphatic endothelial cell poly lactic-co-glycolic acid (plga) |
| title | Conjugation of vascular endothelial growth factor to poly lactic-co-glycolic acid nanospheres enhances differentiation of embryonic stem cells to lymphatic endothelial cells |
| title_full | Conjugation of vascular endothelial growth factor to poly lactic-co-glycolic acid nanospheres enhances differentiation of embryonic stem cells to lymphatic endothelial cells |
| title_fullStr | Conjugation of vascular endothelial growth factor to poly lactic-co-glycolic acid nanospheres enhances differentiation of embryonic stem cells to lymphatic endothelial cells |
| title_full_unstemmed | Conjugation of vascular endothelial growth factor to poly lactic-co-glycolic acid nanospheres enhances differentiation of embryonic stem cells to lymphatic endothelial cells |
| title_short | Conjugation of vascular endothelial growth factor to poly lactic-co-glycolic acid nanospheres enhances differentiation of embryonic stem cells to lymphatic endothelial cells |
| title_sort | conjugation of vascular endothelial growth factor to poly lactic co glycolic acid nanospheres enhances differentiation of embryonic stem cells to lymphatic endothelial cells |
| topic | stem cell lymphatic endothelial cell poly lactic-co-glycolic acid (plga) |
| url | http://www.animbiosci.org/upload/pdf/ajas-20-0202.pdf |
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