Phytochemical Constituents, Antioxidant, Cytotoxic, and Antimicrobial Activities of the Ethanolic Extract of Mexican Brown Propolis
Propolis is a complex mixture of natural sticky and resinous components produced by honeybees from living plant exudates. Globally, research has been dedicated to studying the biological properties and chemical composition of propolis from various geographical and climatic regions. However, the chem...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/1/70 |
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| author | J. Fausto Rivero-Cruz Jessica Granados-Pineda José Pedraza-Chaverri Jazmin Marlen Pérez-Rojas Ajit Kumar-Passari Gloria Diaz-Ruiz Blanca Estela Rivero-Cruz |
| author_facet | J. Fausto Rivero-Cruz Jessica Granados-Pineda José Pedraza-Chaverri Jazmin Marlen Pérez-Rojas Ajit Kumar-Passari Gloria Diaz-Ruiz Blanca Estela Rivero-Cruz |
| author_sort | J. Fausto Rivero-Cruz |
| collection | DOAJ |
| description | Propolis is a complex mixture of natural sticky and resinous components produced by honeybees from living plant exudates. Globally, research has been dedicated to studying the biological properties and chemical composition of propolis from various geographical and climatic regions. However, the chemical data and biological properties of Mexican brown propolis are scant. The antioxidant activity of the ethanolic extract of propolis (EEP) sample collected in México and the isolated compounds is described. Cytotoxic activity was evaluated in a central nervous system and cervical cancer cell lines. Cytotoxicity of EEP was evaluated in a C6 cell line and cervical cancer (HeLa, SiHa, and CasKi) measured by the 3-(3,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium (MTT) assay. The antibacterial activity was tested using the minimum inhibitory concentration (MIC) assay. Twelve known compounds were isolated and identified by nuclear magnetic resonance spectroscopy (NMR). Additionally, forty volatile compounds were identified by means of headspace-solid phase microextraction with gas chromatography and mass spectrometry time of flight analysis (HS-SPME/GC-MS-TOF). The main volatile compounds detected include nonanal (18.82%), α-pinene (12.45%), neryl alcohol (10.13%), and α-pinene (8.04%). EEP showed an anti-proliferative effect on glioma cells better than temozolomide, also decreased proliferation and viability in cervical cancer cells, but its effectiveness was lower compared to cisplatin. |
| first_indexed | 2024-03-12T19:31:33Z |
| format | Article |
| id | doaj.art-744982eb95c54a4096cb41f06f59ee89 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T19:31:33Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-744982eb95c54a4096cb41f06f59ee892023-08-02T04:27:08ZengMDPI AGAntioxidants2076-39212020-01-01917010.3390/antiox9010070antiox9010070Phytochemical Constituents, Antioxidant, Cytotoxic, and Antimicrobial Activities of the Ethanolic Extract of Mexican Brown PropolisJ. Fausto Rivero-Cruz0Jessica Granados-Pineda1José Pedraza-Chaverri2Jazmin Marlen Pérez-Rojas3Ajit Kumar-Passari4Gloria Diaz-Ruiz5Blanca Estela Rivero-Cruz6Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, MexicoFacultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, MexicoFacultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, MexicoDivisión de Investigación Básica, Instituto Nacional de Cancerología, Av. San Fernando 22, Apartado Postal 22026, Tlalpan 14000, MexicoInstituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, MexicoFacultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, MexicoFacultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, MexicoPropolis is a complex mixture of natural sticky and resinous components produced by honeybees from living plant exudates. Globally, research has been dedicated to studying the biological properties and chemical composition of propolis from various geographical and climatic regions. However, the chemical data and biological properties of Mexican brown propolis are scant. The antioxidant activity of the ethanolic extract of propolis (EEP) sample collected in México and the isolated compounds is described. Cytotoxic activity was evaluated in a central nervous system and cervical cancer cell lines. Cytotoxicity of EEP was evaluated in a C6 cell line and cervical cancer (HeLa, SiHa, and CasKi) measured by the 3-(3,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium (MTT) assay. The antibacterial activity was tested using the minimum inhibitory concentration (MIC) assay. Twelve known compounds were isolated and identified by nuclear magnetic resonance spectroscopy (NMR). Additionally, forty volatile compounds were identified by means of headspace-solid phase microextraction with gas chromatography and mass spectrometry time of flight analysis (HS-SPME/GC-MS-TOF). The main volatile compounds detected include nonanal (18.82%), α-pinene (12.45%), neryl alcohol (10.13%), and α-pinene (8.04%). EEP showed an anti-proliferative effect on glioma cells better than temozolomide, also decreased proliferation and viability in cervical cancer cells, but its effectiveness was lower compared to cisplatin.https://www.mdpi.com/2076-3921/9/1/70propolisantioxidant activitycytotoxicantibacterialméxicohs-spme/gc-ms-tofnmrvolatile compoundsflavonoidsphenolic acids |
| spellingShingle | J. Fausto Rivero-Cruz Jessica Granados-Pineda José Pedraza-Chaverri Jazmin Marlen Pérez-Rojas Ajit Kumar-Passari Gloria Diaz-Ruiz Blanca Estela Rivero-Cruz Phytochemical Constituents, Antioxidant, Cytotoxic, and Antimicrobial Activities of the Ethanolic Extract of Mexican Brown Propolis Antioxidants propolis antioxidant activity cytotoxic antibacterial méxico hs-spme/gc-ms-tof nmr volatile compounds flavonoids phenolic acids |
| title | Phytochemical Constituents, Antioxidant, Cytotoxic, and Antimicrobial Activities of the Ethanolic Extract of Mexican Brown Propolis |
| title_full | Phytochemical Constituents, Antioxidant, Cytotoxic, and Antimicrobial Activities of the Ethanolic Extract of Mexican Brown Propolis |
| title_fullStr | Phytochemical Constituents, Antioxidant, Cytotoxic, and Antimicrobial Activities of the Ethanolic Extract of Mexican Brown Propolis |
| title_full_unstemmed | Phytochemical Constituents, Antioxidant, Cytotoxic, and Antimicrobial Activities of the Ethanolic Extract of Mexican Brown Propolis |
| title_short | Phytochemical Constituents, Antioxidant, Cytotoxic, and Antimicrobial Activities of the Ethanolic Extract of Mexican Brown Propolis |
| title_sort | phytochemical constituents antioxidant cytotoxic and antimicrobial activities of the ethanolic extract of mexican brown propolis |
| topic | propolis antioxidant activity cytotoxic antibacterial méxico hs-spme/gc-ms-tof nmr volatile compounds flavonoids phenolic acids |
| url | https://www.mdpi.com/2076-3921/9/1/70 |
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