Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (<i>Helianthus annuus</i> L.)
Rust and downy mildew (DM) are two important sunflower diseases that lead to significant yield losses globally. The use of resistant hybrids to control rust and DM in sunflower has a long history. The rust resistance genes, <i>R<sub>13a</sub></i> and <i>R<sub>16&l...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/17/9516 |
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| author | Guojia Ma Qijian Song Xuehui Li Lili Qi |
| author_facet | Guojia Ma Qijian Song Xuehui Li Lili Qi |
| author_sort | Guojia Ma |
| collection | DOAJ |
| description | Rust and downy mildew (DM) are two important sunflower diseases that lead to significant yield losses globally. The use of resistant hybrids to control rust and DM in sunflower has a long history. The rust resistance genes, <i>R<sub>13a</sub></i> and <i>R<sub>16</sub></i>, were previously mapped to a 3.4 Mb region at the lower end of sunflower chromosome 13, while the DM resistance gene, <i>Pl<sub>33</sub></i>, was previously mapped to a 4.2 Mb region located at the upper end of chromosome 4. High-resolution fine mapping was conducted using whole genome sequencing of HA-R6 (<i>R<sub>13a</sub></i>) and TX16R (<i>R<sub>16</sub></i> and <i>Pl<sub>33</sub></i>) and large segregated populations. <i>R<sub>13a</sub></i> and <i>R<sub>16</sub></i> were fine mapped to a 0.48 cM region in chromosome 13 corresponding to a 790 kb physical interval on the XRQr1.0 genome assembly. Four disease defense-related genes with nucleotide-binding leucine-rich repeat (NLR) motifs were found in this region from XRQr1.0 gene annotation as candidate genes for <i>R<sub>13a</sub></i> and <i>R<sub>16</sub></i>. <i>Pl<sub>33</sub></i> was fine mapped to a 0.04 cM region in chromosome 4 corresponding to a 63 kb physical interval. One NLR gene, HanXRQChr04g0095641, was predicted as the candidate gene for <i>Pl<sub>33</sub></i>. The diagnostic SNP markers developed for each gene in the current study will facilitate marker-assisted selections of resistance genes in sunflower breeding programs. |
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| last_indexed | 2024-03-10T01:48:13Z |
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| spelling | doaj.art-744eba4e65e04a07a2ddd9db75b861572023-11-23T13:12:01ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012317951610.3390/ijms23179516Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (<i>Helianthus annuus</i> L.)Guojia Ma0Qijian Song1Xuehui Li2Lili Qi3Department of Plant Sciences, North Dakota State University, Fargo, ND 58102-6050, USASoybean Genomics and Improvement Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705-2350, USADepartment of Plant Sciences, North Dakota State University, Fargo, ND 58102-6050, USAUSDA-Agricultural Research Service, Edward T. Schafer Agricultural Research Center, Fargo, ND 58102-2765, USARust and downy mildew (DM) are two important sunflower diseases that lead to significant yield losses globally. The use of resistant hybrids to control rust and DM in sunflower has a long history. The rust resistance genes, <i>R<sub>13a</sub></i> and <i>R<sub>16</sub></i>, were previously mapped to a 3.4 Mb region at the lower end of sunflower chromosome 13, while the DM resistance gene, <i>Pl<sub>33</sub></i>, was previously mapped to a 4.2 Mb region located at the upper end of chromosome 4. High-resolution fine mapping was conducted using whole genome sequencing of HA-R6 (<i>R<sub>13a</sub></i>) and TX16R (<i>R<sub>16</sub></i> and <i>Pl<sub>33</sub></i>) and large segregated populations. <i>R<sub>13a</sub></i> and <i>R<sub>16</sub></i> were fine mapped to a 0.48 cM region in chromosome 13 corresponding to a 790 kb physical interval on the XRQr1.0 genome assembly. Four disease defense-related genes with nucleotide-binding leucine-rich repeat (NLR) motifs were found in this region from XRQr1.0 gene annotation as candidate genes for <i>R<sub>13a</sub></i> and <i>R<sub>16</sub></i>. <i>Pl<sub>33</sub></i> was fine mapped to a 0.04 cM region in chromosome 4 corresponding to a 63 kb physical interval. One NLR gene, HanXRQChr04g0095641, was predicted as the candidate gene for <i>Pl<sub>33</sub></i>. The diagnostic SNP markers developed for each gene in the current study will facilitate marker-assisted selections of resistance genes in sunflower breeding programs.https://www.mdpi.com/1422-0067/23/17/9516sunflowerrustdowny mildewresistance genesfine mapping |
| spellingShingle | Guojia Ma Qijian Song Xuehui Li Lili Qi Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (<i>Helianthus annuus</i> L.) International Journal of Molecular Sciences sunflower rust downy mildew resistance genes fine mapping |
| title | Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (<i>Helianthus annuus</i> L.) |
| title_full | Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (<i>Helianthus annuus</i> L.) |
| title_fullStr | Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (<i>Helianthus annuus</i> L.) |
| title_full_unstemmed | Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (<i>Helianthus annuus</i> L.) |
| title_short | Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (<i>Helianthus annuus</i> L.) |
| title_sort | genetic insight into disease resistance gene clusters by using sequencing based fine mapping in sunflower i helianthus annuus i l |
| topic | sunflower rust downy mildew resistance genes fine mapping |
| url | https://www.mdpi.com/1422-0067/23/17/9516 |
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