Alteration of Mitochondrial Transcript Expression in <i>Arabidopsis thaliana</i> Using a Custom-Made Library of Pentatricopeptide Repeat Proteins
Pentatricopeptide repeat (PPR) proteins are considered a potential tool for manipulating organelle gene expression in plants because they can recognise a wide range of different RNA sequences, and the molecular basis for this sequence recognition is partially known and understood. A library of redes...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/17/13233 |
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| author | Lilian Vincis Pereira Sanglard Ian D. Small Catherine Colas des Francs-Small |
| author_facet | Lilian Vincis Pereira Sanglard Ian D. Small Catherine Colas des Francs-Small |
| author_sort | Lilian Vincis Pereira Sanglard |
| collection | DOAJ |
| description | Pentatricopeptide repeat (PPR) proteins are considered a potential tool for manipulating organelle gene expression in plants because they can recognise a wide range of different RNA sequences, and the molecular basis for this sequence recognition is partially known and understood. A library of redesigned PPR proteins related to restorer-of-fertility proteins was created and transformed into plants in order to target mitochondrial transcripts. Ninety different variants tested in vivo showed a wide range of phenotypes. One of these lines, which displayed slow growth and downward curled leaves, showed a clear reduction in complex V. The phenotype was due to a specific cleavage of <i>atp1</i> transcripts induced by a modified PPR protein from the library, validating the use of this library as a source of mitochondrial ‘mutants’. This study is a step towards developing specific RNA targeting tools using PPR proteins that can be aimed at desired targets. |
| first_indexed | 2024-03-10T23:21:29Z |
| format | Article |
| id | doaj.art-744fd7ade30a4f49ae3d3d5b630879bf |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:21:29Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-744fd7ade30a4f49ae3d3d5b630879bf2023-11-19T08:14:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124171323310.3390/ijms241713233Alteration of Mitochondrial Transcript Expression in <i>Arabidopsis thaliana</i> Using a Custom-Made Library of Pentatricopeptide Repeat ProteinsLilian Vincis Pereira Sanglard0Ian D. Small1Catherine Colas des Francs-Small2Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, Crawley, WA 6009, AustraliaAustralian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, Crawley, WA 6009, AustraliaAustralian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, Crawley, WA 6009, AustraliaPentatricopeptide repeat (PPR) proteins are considered a potential tool for manipulating organelle gene expression in plants because they can recognise a wide range of different RNA sequences, and the molecular basis for this sequence recognition is partially known and understood. A library of redesigned PPR proteins related to restorer-of-fertility proteins was created and transformed into plants in order to target mitochondrial transcripts. Ninety different variants tested in vivo showed a wide range of phenotypes. One of these lines, which displayed slow growth and downward curled leaves, showed a clear reduction in complex V. The phenotype was due to a specific cleavage of <i>atp1</i> transcripts induced by a modified PPR protein from the library, validating the use of this library as a source of mitochondrial ‘mutants’. This study is a step towards developing specific RNA targeting tools using PPR proteins that can be aimed at desired targets.https://www.mdpi.com/1422-0067/24/17/13233plant mitochondriapentatricopeptide repeat (PPR) proteinsATP synthaseRNA processing factor 2 (RPF2)biotechnology |
| spellingShingle | Lilian Vincis Pereira Sanglard Ian D. Small Catherine Colas des Francs-Small Alteration of Mitochondrial Transcript Expression in <i>Arabidopsis thaliana</i> Using a Custom-Made Library of Pentatricopeptide Repeat Proteins International Journal of Molecular Sciences plant mitochondria pentatricopeptide repeat (PPR) proteins ATP synthase RNA processing factor 2 (RPF2) biotechnology |
| title | Alteration of Mitochondrial Transcript Expression in <i>Arabidopsis thaliana</i> Using a Custom-Made Library of Pentatricopeptide Repeat Proteins |
| title_full | Alteration of Mitochondrial Transcript Expression in <i>Arabidopsis thaliana</i> Using a Custom-Made Library of Pentatricopeptide Repeat Proteins |
| title_fullStr | Alteration of Mitochondrial Transcript Expression in <i>Arabidopsis thaliana</i> Using a Custom-Made Library of Pentatricopeptide Repeat Proteins |
| title_full_unstemmed | Alteration of Mitochondrial Transcript Expression in <i>Arabidopsis thaliana</i> Using a Custom-Made Library of Pentatricopeptide Repeat Proteins |
| title_short | Alteration of Mitochondrial Transcript Expression in <i>Arabidopsis thaliana</i> Using a Custom-Made Library of Pentatricopeptide Repeat Proteins |
| title_sort | alteration of mitochondrial transcript expression in i arabidopsis thaliana i using a custom made library of pentatricopeptide repeat proteins |
| topic | plant mitochondria pentatricopeptide repeat (PPR) proteins ATP synthase RNA processing factor 2 (RPF2) biotechnology |
| url | https://www.mdpi.com/1422-0067/24/17/13233 |
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