Genomic Selection for F1 Hybrid Breeding in Strawberry (Fragaria × ananassa)
Cultivated strawberry is the most widely consumed fruit crop in the world, and therefore, many breeding programs are underway to improve its agronomic traits such as fruit quality. Strawberry cultivars were vegetatively propagated through runners and carried a high risk of infection with viruses and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.645111/full |
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| author | Eiji Yamamoto Sono Kataoka Kenta Shirasawa Yuji Noguchi Sachiko Isobe |
| author_facet | Eiji Yamamoto Sono Kataoka Kenta Shirasawa Yuji Noguchi Sachiko Isobe |
| author_sort | Eiji Yamamoto |
| collection | DOAJ |
| description | Cultivated strawberry is the most widely consumed fruit crop in the world, and therefore, many breeding programs are underway to improve its agronomic traits such as fruit quality. Strawberry cultivars were vegetatively propagated through runners and carried a high risk of infection with viruses and insects. To solve this problem, the development of F1 hybrid seeds has been proposed as an alternative breeding strategy in strawberry. In this study, we conducted a potential assessment of genomic selection (GS) in strawberry F1 hybrid breeding. A total of 105 inbred lines were developed as candidate parents of strawberry F1 hybrids. In addition, 275 parental combinations were randomly selected from the 105 inbred lines and crossed to develop test F1 hybrids for GS model training. These populations were phenotyped for petiole length, leaf area, Brix, fruit hardness, and pericarp color. Whole-genome shotgun sequencing of the 105 inbred lines detected 20,811 single nucleotide polymorphism sites that were provided for subsequent GS analyses. In a GS model construction, inclusion of dominant effects showed a slight advantage in GS accuracy. In the across population prediction analysis, GS models using the inbred lines showed predictability for the test F1 hybrids and vice versa, except for Brix. Finally, the GS models were used for phenotype prediction of 5,460 possible F1 hybrids from 105 inbred lines to select F1 hybrids with high fruit hardness or high pericarp color. These F1 hybrids were developed and phenotyped to evaluate the efficacy of the GS. As expected, F1 hybrids that were predicted to have high fruit hardness or high pericarp color expressed higher observed phenotypic values than the F1 hybrids that were selected for other objectives. Through the analyses in this study, we demonstrated that GS can be applied for strawberry F1 hybrid breeding. |
| first_indexed | 2024-12-16T15:15:08Z |
| format | Article |
| id | doaj.art-7451ebc25a7b45faa6c24bd1f77c4c59 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-16T15:15:08Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7451ebc25a7b45faa6c24bd1f77c4c592022-12-21T22:26:49ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-03-011210.3389/fpls.2021.645111645111Genomic Selection for F1 Hybrid Breeding in Strawberry (Fragaria × ananassa)Eiji Yamamoto0Sono Kataoka1Kenta Shirasawa2Yuji Noguchi3Sachiko Isobe4Graduate School of Agriculture, Meiji University, Kawasaki, JapanInstitute of Vegetable and Floriculture Science, National Agriculture and Food Research Organization, Tsu, JapanDepartment of Frontier Research and Development, Kazusa DNA Research Institute, Kisarazu, JapanInstitute of Vegetable and Floriculture Science, National Agriculture and Food Research Organization, Tsu, JapanDepartment of Frontier Research and Development, Kazusa DNA Research Institute, Kisarazu, JapanCultivated strawberry is the most widely consumed fruit crop in the world, and therefore, many breeding programs are underway to improve its agronomic traits such as fruit quality. Strawberry cultivars were vegetatively propagated through runners and carried a high risk of infection with viruses and insects. To solve this problem, the development of F1 hybrid seeds has been proposed as an alternative breeding strategy in strawberry. In this study, we conducted a potential assessment of genomic selection (GS) in strawberry F1 hybrid breeding. A total of 105 inbred lines were developed as candidate parents of strawberry F1 hybrids. In addition, 275 parental combinations were randomly selected from the 105 inbred lines and crossed to develop test F1 hybrids for GS model training. These populations were phenotyped for petiole length, leaf area, Brix, fruit hardness, and pericarp color. Whole-genome shotgun sequencing of the 105 inbred lines detected 20,811 single nucleotide polymorphism sites that were provided for subsequent GS analyses. In a GS model construction, inclusion of dominant effects showed a slight advantage in GS accuracy. In the across population prediction analysis, GS models using the inbred lines showed predictability for the test F1 hybrids and vice versa, except for Brix. Finally, the GS models were used for phenotype prediction of 5,460 possible F1 hybrids from 105 inbred lines to select F1 hybrids with high fruit hardness or high pericarp color. These F1 hybrids were developed and phenotyped to evaluate the efficacy of the GS. As expected, F1 hybrids that were predicted to have high fruit hardness or high pericarp color expressed higher observed phenotypic values than the F1 hybrids that were selected for other objectives. Through the analyses in this study, we demonstrated that GS can be applied for strawberry F1 hybrid breeding.https://www.frontiersin.org/articles/10.3389/fpls.2021.645111/fullstrawberryF1 hybrid breedinggenomic selectionfruit hardnesspericarp color |
| spellingShingle | Eiji Yamamoto Sono Kataoka Kenta Shirasawa Yuji Noguchi Sachiko Isobe Genomic Selection for F1 Hybrid Breeding in Strawberry (Fragaria × ananassa) Frontiers in Plant Science strawberry F1 hybrid breeding genomic selection fruit hardness pericarp color |
| title | Genomic Selection for F1 Hybrid Breeding in Strawberry (Fragaria × ananassa) |
| title_full | Genomic Selection for F1 Hybrid Breeding in Strawberry (Fragaria × ananassa) |
| title_fullStr | Genomic Selection for F1 Hybrid Breeding in Strawberry (Fragaria × ananassa) |
| title_full_unstemmed | Genomic Selection for F1 Hybrid Breeding in Strawberry (Fragaria × ananassa) |
| title_short | Genomic Selection for F1 Hybrid Breeding in Strawberry (Fragaria × ananassa) |
| title_sort | genomic selection for f1 hybrid breeding in strawberry fragaria ananassa |
| topic | strawberry F1 hybrid breeding genomic selection fruit hardness pericarp color |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.645111/full |
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