Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of <i>Pulicaria incisa</i> (Lam.) DC Inherent to Arid Regions
In this study, 15 bacterial endophytes linked with the leaves of the native medicinal plant <i>Pulicaria incisa</i> were isolated and identified as <i>Agrobacterium fabrum</i>, <i>Acinetobacter radioresistant</i>, <i>Brevibacillus brevis</i>, <i>...
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MDPI AG
2021-01-01
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| author | Amr Fouda Ahmed M. Eid Albaraa Elsaied Ehab F. El-Belely Mohammed G. Barghoth Ehab Azab Adil A. Gobouri Saad El-Din Hassan |
| author_facet | Amr Fouda Ahmed M. Eid Albaraa Elsaied Ehab F. El-Belely Mohammed G. Barghoth Ehab Azab Adil A. Gobouri Saad El-Din Hassan |
| author_sort | Amr Fouda |
| collection | DOAJ |
| description | In this study, 15 bacterial endophytes linked with the leaves of the native medicinal plant <i>Pulicaria incisa</i> were isolated and identified as <i>Agrobacterium fabrum</i>, <i>Acinetobacter radioresistant</i>, <i>Brevibacillus brevis</i>, <i>Bacillus cereus</i>, <i>Bacillus subtilis</i>, <i>Paenibacillus barengoltzii</i>, and <i>Burkholderia cepacia.</i> These isolates exhibited variant tolerances to salt stress and showed high efficacy in indole-3-acetic acid (IAA) production in the absence/presence of tryptophan. The maximum productivity of IAA was recorded for <i>B. cereus</i> BI-8 and <i>B. subtilis</i> BI-10 with values of 117 ± 6 and 108 ± 4.6 μg mL<sup>−1</sup>, respectively, in the presence of 5 mg mL<sup>−1</sup> tryptophan after 10 days. These two isolates had a high potential in phosphate solubilization and ammonia production, and they showed enzymatic activities for amylase, protease, xylanase, cellulase, chitinase, and catalase. In vitro antagonistic investigation showed their high efficacy against the three phytopathogens <i>Fusarium oxysporum</i>, <i>Alternaria alternata</i>, and <i>Pythium ultimum</i>, with inhibition percentages ranging from 20% ± 0.2% to 52.6% ± 0.2% (<i>p</i> ≤ 0.05). Therefore, these two endophytic bacteria were used as bio-inoculants for maize seeds, and the results showed that bacterial inoculations significantly increased the root length as well as the fresh and dry weights of the roots compared to the control plants. The <i>Zea mays</i> plant inoculated with the two endophytic strains BI-8 and BI-10 significantly improved (<i>p</i> ≤ 0.05) the growth performance as well as the nutrient uptake compared with an un-inoculated plant. |
| first_indexed | 2024-03-10T13:33:56Z |
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| institution | Directory Open Access Journal |
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| spelling | doaj.art-745275dfab9e49e1b3a3aa018e4b4f972023-11-21T07:41:38ZengMDPI AGPlants2223-77472021-01-011017610.3390/plants10010076Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of <i>Pulicaria incisa</i> (Lam.) DC Inherent to Arid RegionsAmr Fouda0Ahmed M. Eid1Albaraa Elsaied2Ehab F. El-Belely3Mohammed G. Barghoth4Ehab Azab5Adil A. Gobouri6Saad El-Din Hassan7Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptIn this study, 15 bacterial endophytes linked with the leaves of the native medicinal plant <i>Pulicaria incisa</i> were isolated and identified as <i>Agrobacterium fabrum</i>, <i>Acinetobacter radioresistant</i>, <i>Brevibacillus brevis</i>, <i>Bacillus cereus</i>, <i>Bacillus subtilis</i>, <i>Paenibacillus barengoltzii</i>, and <i>Burkholderia cepacia.</i> These isolates exhibited variant tolerances to salt stress and showed high efficacy in indole-3-acetic acid (IAA) production in the absence/presence of tryptophan. The maximum productivity of IAA was recorded for <i>B. cereus</i> BI-8 and <i>B. subtilis</i> BI-10 with values of 117 ± 6 and 108 ± 4.6 μg mL<sup>−1</sup>, respectively, in the presence of 5 mg mL<sup>−1</sup> tryptophan after 10 days. These two isolates had a high potential in phosphate solubilization and ammonia production, and they showed enzymatic activities for amylase, protease, xylanase, cellulase, chitinase, and catalase. In vitro antagonistic investigation showed their high efficacy against the three phytopathogens <i>Fusarium oxysporum</i>, <i>Alternaria alternata</i>, and <i>Pythium ultimum</i>, with inhibition percentages ranging from 20% ± 0.2% to 52.6% ± 0.2% (<i>p</i> ≤ 0.05). Therefore, these two endophytic bacteria were used as bio-inoculants for maize seeds, and the results showed that bacterial inoculations significantly increased the root length as well as the fresh and dry weights of the roots compared to the control plants. The <i>Zea mays</i> plant inoculated with the two endophytic strains BI-8 and BI-10 significantly improved (<i>p</i> ≤ 0.05) the growth performance as well as the nutrient uptake compared with an un-inoculated plant.https://www.mdpi.com/2223-7747/10/1/76<i>Pulicaria incisa</i>bacterial endophytesplant growth-promotingIAA productionantagonistic activity<i>Zea mays</i> |
| spellingShingle | Amr Fouda Ahmed M. Eid Albaraa Elsaied Ehab F. El-Belely Mohammed G. Barghoth Ehab Azab Adil A. Gobouri Saad El-Din Hassan Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of <i>Pulicaria incisa</i> (Lam.) DC Inherent to Arid Regions Plants <i>Pulicaria incisa</i> bacterial endophytes plant growth-promoting IAA production antagonistic activity <i>Zea mays</i> |
| title | Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of <i>Pulicaria incisa</i> (Lam.) DC Inherent to Arid Regions |
| title_full | Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of <i>Pulicaria incisa</i> (Lam.) DC Inherent to Arid Regions |
| title_fullStr | Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of <i>Pulicaria incisa</i> (Lam.) DC Inherent to Arid Regions |
| title_full_unstemmed | Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of <i>Pulicaria incisa</i> (Lam.) DC Inherent to Arid Regions |
| title_short | Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of <i>Pulicaria incisa</i> (Lam.) DC Inherent to Arid Regions |
| title_sort | plant growth promoting endophytic bacterial community inhabiting the leaves of i pulicaria incisa i lam dc inherent to arid regions |
| topic | <i>Pulicaria incisa</i> bacterial endophytes plant growth-promoting IAA production antagonistic activity <i>Zea mays</i> |
| url | https://www.mdpi.com/2223-7747/10/1/76 |
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