Light driven CO2 fixation by using cyanobacterial photosystem I and NADPH-dependent formate dehydrogenase.
The ultimate goal of this research is to construct a new direct CO2 fixation system using photosystems in living algae. Here, we report light-driven formate production from CO2 by using cyanobacterial photosystem I (PS I). Formate, a chemical hydrogen carrier and important industrial material, can b...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3735542?pdf=render |
| _version_ | 1811321541236883456 |
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| author | Masaki Ihara Yusuke Kawano Miho Urano Ayako Okabe |
| author_facet | Masaki Ihara Yusuke Kawano Miho Urano Ayako Okabe |
| author_sort | Masaki Ihara |
| collection | DOAJ |
| description | The ultimate goal of this research is to construct a new direct CO2 fixation system using photosystems in living algae. Here, we report light-driven formate production from CO2 by using cyanobacterial photosystem I (PS I). Formate, a chemical hydrogen carrier and important industrial material, can be produced from CO2 by using the reducing power and the catalytic function of formate dehydrogenase (FDH). We created a bacterial FDH mutant that experimentally switched the cofactor specificity from NADH to NADPH, and combined it with an in vitro-reconstituted cyanobacterial light-driven NADPH production system consisting of PS I, ferredoxin (Fd), and ferredoxin-NADP(+)-reductase (FNR). Consequently, light-dependent formate production under a CO2 atmosphere was successfully achieved. In addition, we introduced the NADPH-dependent FDH mutant into heterocysts of the cyanobacterium Anabaena sp. PCC 7120 and demonstrated an increased formate concentration in the cells. These results provide a new possibility for photo-biological CO2 fixation. |
| first_indexed | 2024-04-13T13:19:07Z |
| format | Article |
| id | doaj.art-7452cc792bef43cb88b7a9e83d84eeb5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T13:19:07Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7452cc792bef43cb88b7a9e83d84eeb52022-12-22T02:45:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7158110.1371/journal.pone.0071581Light driven CO2 fixation by using cyanobacterial photosystem I and NADPH-dependent formate dehydrogenase.Masaki IharaYusuke KawanoMiho UranoAyako OkabeThe ultimate goal of this research is to construct a new direct CO2 fixation system using photosystems in living algae. Here, we report light-driven formate production from CO2 by using cyanobacterial photosystem I (PS I). Formate, a chemical hydrogen carrier and important industrial material, can be produced from CO2 by using the reducing power and the catalytic function of formate dehydrogenase (FDH). We created a bacterial FDH mutant that experimentally switched the cofactor specificity from NADH to NADPH, and combined it with an in vitro-reconstituted cyanobacterial light-driven NADPH production system consisting of PS I, ferredoxin (Fd), and ferredoxin-NADP(+)-reductase (FNR). Consequently, light-dependent formate production under a CO2 atmosphere was successfully achieved. In addition, we introduced the NADPH-dependent FDH mutant into heterocysts of the cyanobacterium Anabaena sp. PCC 7120 and demonstrated an increased formate concentration in the cells. These results provide a new possibility for photo-biological CO2 fixation.http://europepmc.org/articles/PMC3735542?pdf=render |
| spellingShingle | Masaki Ihara Yusuke Kawano Miho Urano Ayako Okabe Light driven CO2 fixation by using cyanobacterial photosystem I and NADPH-dependent formate dehydrogenase. PLoS ONE |
| title | Light driven CO2 fixation by using cyanobacterial photosystem I and NADPH-dependent formate dehydrogenase. |
| title_full | Light driven CO2 fixation by using cyanobacterial photosystem I and NADPH-dependent formate dehydrogenase. |
| title_fullStr | Light driven CO2 fixation by using cyanobacterial photosystem I and NADPH-dependent formate dehydrogenase. |
| title_full_unstemmed | Light driven CO2 fixation by using cyanobacterial photosystem I and NADPH-dependent formate dehydrogenase. |
| title_short | Light driven CO2 fixation by using cyanobacterial photosystem I and NADPH-dependent formate dehydrogenase. |
| title_sort | light driven co2 fixation by using cyanobacterial photosystem i and nadph dependent formate dehydrogenase |
| url | http://europepmc.org/articles/PMC3735542?pdf=render |
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