Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydans
The characteristics and kinetics of whole-cell catalysis were investigated individually or simultaneously relative to the bio-oxidation of five monosaccharides to corresponding aldonic acids using Gluconobacter oxydans. For individual catalysis, 30 g/L glucose could be consumed completely at 3.21 g/...
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North Carolina State University
2015-05-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_4277_Zhou_Aldonic_Acids_Production |
| _version_ | 1818512673367654400 |
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| author | Xin Zhou Xing Wang Rou Cao Yuheng Tao Yong Xu Shiyuan Yu |
| author_facet | Xin Zhou Xing Wang Rou Cao Yuheng Tao Yong Xu Shiyuan Yu |
| author_sort | Xin Zhou |
| collection | DOAJ |
| description | The characteristics and kinetics of whole-cell catalysis were investigated individually or simultaneously relative to the bio-oxidation of five monosaccharides to corresponding aldonic acids using Gluconobacter oxydans. For individual catalysis, 30 g/L glucose could be consumed completely at 3.21 g/L/h in 8 h, and arabinose was the slowest-consumed at 1.18 g/L/h in 8 h. Among five monosaccharides, the yield of xylonic acid was highest at 90.4%, and about 31.0 g/L xylonic acid could be obtained in 24 h. For simultaneous catalysis, a complex substrate competition appeared in the mixed-aldoses solution. The utilization rate of arabinose, galactose, xylose and mannose were repressed distinctly. And glucose exhibited variable inhibitory effects on the remaining four monosaccharides utilization. Higher concentration of mixed aldoses showed a tendency to minimize the difference of glucose inhibition on arabinose, galactose, xylose, and mannose conversion to corresponding acids. Thus, the total concentration and proportion between various aldoses should be properly controlled for a highly efficient production of aldonic acids from lignocellulosic material. |
| first_indexed | 2024-12-10T23:50:09Z |
| format | Article |
| id | doaj.art-7453ceb5a7944a94ba0455a5edd6a4b9 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-10T23:50:09Z |
| publishDate | 2015-05-01 |
| publisher | North Carolina State University |
| record_format | Article |
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| spelling | doaj.art-7453ceb5a7944a94ba0455a5edd6a4b92022-12-22T01:28:47ZengNorth Carolina State UniversityBioResources1930-21261930-21262015-05-011034277428610.15376/biores.10.3.4277-4286Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydansXin Zhou0Xing Wang1Rou Cao2Yuheng Tao3Yong Xu4Shiyuan Yu5College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu, China; Jiangsu Key Laboratory of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing, Jiangsu, China; ChinaCollege of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu, China; ChinaCollege of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu, China; ChinaCollege of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu, China; ChinaCollege of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu, China; Jiangsu Key Laboratory of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing, Jiangsu, China; ChinaCollege of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu, China; Jiangsu Key Laboratory of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing, Jiangsu, China; ChinaThe characteristics and kinetics of whole-cell catalysis were investigated individually or simultaneously relative to the bio-oxidation of five monosaccharides to corresponding aldonic acids using Gluconobacter oxydans. For individual catalysis, 30 g/L glucose could be consumed completely at 3.21 g/L/h in 8 h, and arabinose was the slowest-consumed at 1.18 g/L/h in 8 h. Among five monosaccharides, the yield of xylonic acid was highest at 90.4%, and about 31.0 g/L xylonic acid could be obtained in 24 h. For simultaneous catalysis, a complex substrate competition appeared in the mixed-aldoses solution. The utilization rate of arabinose, galactose, xylose and mannose were repressed distinctly. And glucose exhibited variable inhibitory effects on the remaining four monosaccharides utilization. Higher concentration of mixed aldoses showed a tendency to minimize the difference of glucose inhibition on arabinose, galactose, xylose, and mannose conversion to corresponding acids. Thus, the total concentration and proportion between various aldoses should be properly controlled for a highly efficient production of aldonic acids from lignocellulosic material.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_4277_Zhou_Aldonic_Acids_ProductionAldonic acidsCatalyticPlant monosaccharidesGluconobacter oxydansCharacteristics and kinetics of bio-catalysis |
| spellingShingle | Xin Zhou Xing Wang Rou Cao Yuheng Tao Yong Xu Shiyuan Yu Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydans BioResources Aldonic acids Catalytic Plant monosaccharides Gluconobacter oxydans Characteristics and kinetics of bio-catalysis |
| title | Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydans |
| title_full | Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydans |
| title_fullStr | Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydans |
| title_full_unstemmed | Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydans |
| title_short | Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydans |
| title_sort | characteristics and kinetics of the aldonic acids production using whole cell catalysis of gluconobacter oxydans |
| topic | Aldonic acids Catalytic Plant monosaccharides Gluconobacter oxydans Characteristics and kinetics of bio-catalysis |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_4277_Zhou_Aldonic_Acids_Production |
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