Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress
Drought and heat are two major stresses predicted to increase in the future due to climate change. Plants exposed to multiple stressors elicit unique responses from those observed under individual stresses. A comparative transcriptome analysis of <i>Lolium temulentum</i> exposed to droug...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/11/2247 |
| _version_ | 1827675585589018624 |
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| author | Ruth C. Martin Brent A. Kronmiller James E. Dombrowski |
| author_facet | Ruth C. Martin Brent A. Kronmiller James E. Dombrowski |
| author_sort | Ruth C. Martin |
| collection | DOAJ |
| description | Drought and heat are two major stresses predicted to increase in the future due to climate change. Plants exposed to multiple stressors elicit unique responses from those observed under individual stresses. A comparative transcriptome analysis of <i>Lolium temulentum</i> exposed to drought plus heat and non-stressed control plants revealed 20,221 unique up-regulated and 17,034 unique down-regulated differentially regulated transcripts. Gene ontology analysis revealed a strong emphasis on transcriptional regulation, protein folding, cell cycle/parts, organelles, binding, transport, signaling, oxidoreductase, and antioxidant activity. Differentially expressed genes (DEGs) encoding for transcriptional control proteins such as basic leucine zipper, APETALA2/Ethylene Responsive Factor, NAC, and WRKY transcription factors, and Zinc Finger (CCCH type and others) proteins were more often up-regulated, while DEGs encoding Basic Helix-Loop-Helix, MYB and GATA transcription factors, and C2H2 type Zinc Finger proteins were more often down-regulated. The DEGs encoding heat shock transcription factors were only up-regulated. Of the hormones, auxin-related DEGs were the most prevalent, encoding for auxin response factors, binding proteins, and efflux/influx carriers. Gibberellin-, cytokinin- and ABA-related DEGs were also prevalent, with fewer DEGs related to jasmonates and brassinosteroids. Knowledge of genes/pathways that grasses use to respond to the combination of heat/drought will be useful in developing multi-stress resistant grasses. |
| first_indexed | 2024-03-10T05:08:19Z |
| format | Article |
| id | doaj.art-74540539b6684c969b1736694e13ed5a |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T05:08:19Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-74540539b6684c969b1736694e13ed5a2023-11-23T01:02:20ZengMDPI AGPlants2223-77472021-10-011011224710.3390/plants10112247Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat StressRuth C. Martin0Brent A. Kronmiller1James E. Dombrowski2USDA-ARS, National Forage Seed Production Research Center, 3450 SW Campus Way, Corvallis, OR 97331-7102, USACenter for Quantitative Life Sciences, Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331-7102, USAUSDA-ARS, National Forage Seed Production Research Center, 3450 SW Campus Way, Corvallis, OR 97331-7102, USADrought and heat are two major stresses predicted to increase in the future due to climate change. Plants exposed to multiple stressors elicit unique responses from those observed under individual stresses. A comparative transcriptome analysis of <i>Lolium temulentum</i> exposed to drought plus heat and non-stressed control plants revealed 20,221 unique up-regulated and 17,034 unique down-regulated differentially regulated transcripts. Gene ontology analysis revealed a strong emphasis on transcriptional regulation, protein folding, cell cycle/parts, organelles, binding, transport, signaling, oxidoreductase, and antioxidant activity. Differentially expressed genes (DEGs) encoding for transcriptional control proteins such as basic leucine zipper, APETALA2/Ethylene Responsive Factor, NAC, and WRKY transcription factors, and Zinc Finger (CCCH type and others) proteins were more often up-regulated, while DEGs encoding Basic Helix-Loop-Helix, MYB and GATA transcription factors, and C2H2 type Zinc Finger proteins were more often down-regulated. The DEGs encoding heat shock transcription factors were only up-regulated. Of the hormones, auxin-related DEGs were the most prevalent, encoding for auxin response factors, binding proteins, and efflux/influx carriers. Gibberellin-, cytokinin- and ABA-related DEGs were also prevalent, with fewer DEGs related to jasmonates and brassinosteroids. Knowledge of genes/pathways that grasses use to respond to the combination of heat/drought will be useful in developing multi-stress resistant grasses.https://www.mdpi.com/2223-7747/10/11/2247abiotic stressdrought stressheat stresshormones<i>Lolium temulentum</i>RNA-sequencing |
| spellingShingle | Ruth C. Martin Brent A. Kronmiller James E. Dombrowski Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress Plants abiotic stress drought stress heat stress hormones <i>Lolium temulentum</i> RNA-sequencing |
| title | Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress |
| title_full | Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress |
| title_fullStr | Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress |
| title_full_unstemmed | Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress |
| title_short | Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress |
| title_sort | transcriptome analysis of i lolium temulentum i exposed to a combination of drought and heat stress |
| topic | abiotic stress drought stress heat stress hormones <i>Lolium temulentum</i> RNA-sequencing |
| url | https://www.mdpi.com/2223-7747/10/11/2247 |
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