Morphological, physiological, and transcriptional responses to low nitrogen stress in Populus deltoides Marsh. clones with contrasting nitrogen use efficiency
Abstract Background Nitrogen (N) is one of the main factors limiting the wood yield in poplar cultivation. Understanding the molecular mechanism of N utilization could play a guiding role in improving the nitrogen use efficiency (NUE) of poplar. Results In this study, three N-efficient genotypes (A1...
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BMC
2021-09-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07991-7 |
| _version_ | 1818676823320428544 |
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| author | Cun Chen Yanguang Chu Qinjun Huang Weixi Zhang Changjun Ding Jing Zhang Bo Li Tengqian Zhang Zhenghong Li Xiaohua Su |
| author_facet | Cun Chen Yanguang Chu Qinjun Huang Weixi Zhang Changjun Ding Jing Zhang Bo Li Tengqian Zhang Zhenghong Li Xiaohua Su |
| author_sort | Cun Chen |
| collection | DOAJ |
| description | Abstract Background Nitrogen (N) is one of the main factors limiting the wood yield in poplar cultivation. Understanding the molecular mechanism of N utilization could play a guiding role in improving the nitrogen use efficiency (NUE) of poplar. Results In this study, three N-efficient genotypes (A1-A3) and three N-inefficient genotypes (C1-C3) of Populus deltoides were cultured under low N stress (5 μM NH4NO3) and normal N supply (750 μM NH4NO3). The dry matter mass, leaf morphology, and chlorophyll content of both genotypes decreased under N starvation. The low nitrogen adaptation coefficients of the leaves and stems biomass of group A were significantly higher than those of group C (p < 0.05). Interestingly, N starvation induced fine root growth in group A, but not in group C. Next, a detailed time-course analysis of enzyme activities and gene expression in leaves identified 2062 specifically differentially expressed genes (DEGs) in group A and 1118 in group C. Moreover, the sensitivity to N starvation of group A was weak, and DEGs related to hormone signal transduction and stimulus response played an important role in the low N response this group. Weighted gene co-expression network analysis identified genes related to membranes, catalytic activity, enzymatic activity, and response to stresses that might be critical for poplar’s adaption to N starvation and these genes participated in the negative regulation of various biological processes. Finally, ten influential hub genes and twelve transcription factors were identified in the response to N starvation. Among them, four hub genes were related to programmed cell death and the defense response, and PodelWRKY18, with high connectivity, was involved in plant signal transduction. The expression of hub genes increased gradually with the extension of low N stress time, and the expression changes in group A were more obvious than those in group C. Conclusions Under N starvation, group A showed stronger adaptability and better NUE than group C in terms of morphology and physiology. The discovery of hub genes and transcription factors might provide new information for the analysis of the molecular mechanism of NUE and its improvement in poplar. |
| first_indexed | 2024-12-17T08:49:36Z |
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| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-17T08:49:36Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj.art-7457ad756b284807a6198169e87544b22022-12-21T21:56:07ZengBMCBMC Genomics1471-21642021-09-0122111810.1186/s12864-021-07991-7Morphological, physiological, and transcriptional responses to low nitrogen stress in Populus deltoides Marsh. clones with contrasting nitrogen use efficiencyCun Chen0Yanguang Chu1Qinjun Huang2Weixi Zhang3Changjun Ding4Jing Zhang5Bo Li6Tengqian Zhang7Zhenghong Li8Xiaohua Su9State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of ForestryAbstract Background Nitrogen (N) is one of the main factors limiting the wood yield in poplar cultivation. Understanding the molecular mechanism of N utilization could play a guiding role in improving the nitrogen use efficiency (NUE) of poplar. Results In this study, three N-efficient genotypes (A1-A3) and three N-inefficient genotypes (C1-C3) of Populus deltoides were cultured under low N stress (5 μM NH4NO3) and normal N supply (750 μM NH4NO3). The dry matter mass, leaf morphology, and chlorophyll content of both genotypes decreased under N starvation. The low nitrogen adaptation coefficients of the leaves and stems biomass of group A were significantly higher than those of group C (p < 0.05). Interestingly, N starvation induced fine root growth in group A, but not in group C. Next, a detailed time-course analysis of enzyme activities and gene expression in leaves identified 2062 specifically differentially expressed genes (DEGs) in group A and 1118 in group C. Moreover, the sensitivity to N starvation of group A was weak, and DEGs related to hormone signal transduction and stimulus response played an important role in the low N response this group. Weighted gene co-expression network analysis identified genes related to membranes, catalytic activity, enzymatic activity, and response to stresses that might be critical for poplar’s adaption to N starvation and these genes participated in the negative regulation of various biological processes. Finally, ten influential hub genes and twelve transcription factors were identified in the response to N starvation. Among them, four hub genes were related to programmed cell death and the defense response, and PodelWRKY18, with high connectivity, was involved in plant signal transduction. The expression of hub genes increased gradually with the extension of low N stress time, and the expression changes in group A were more obvious than those in group C. Conclusions Under N starvation, group A showed stronger adaptability and better NUE than group C in terms of morphology and physiology. The discovery of hub genes and transcription factors might provide new information for the analysis of the molecular mechanism of NUE and its improvement in poplar.https://doi.org/10.1186/s12864-021-07991-7Nitrogen deficiencyNitrogen use efficiencyGene expressionPopulus deltoides Marsh. |
| spellingShingle | Cun Chen Yanguang Chu Qinjun Huang Weixi Zhang Changjun Ding Jing Zhang Bo Li Tengqian Zhang Zhenghong Li Xiaohua Su Morphological, physiological, and transcriptional responses to low nitrogen stress in Populus deltoides Marsh. clones with contrasting nitrogen use efficiency BMC Genomics Nitrogen deficiency Nitrogen use efficiency Gene expression Populus deltoides Marsh. |
| title | Morphological, physiological, and transcriptional responses to low nitrogen stress in Populus deltoides Marsh. clones with contrasting nitrogen use efficiency |
| title_full | Morphological, physiological, and transcriptional responses to low nitrogen stress in Populus deltoides Marsh. clones with contrasting nitrogen use efficiency |
| title_fullStr | Morphological, physiological, and transcriptional responses to low nitrogen stress in Populus deltoides Marsh. clones with contrasting nitrogen use efficiency |
| title_full_unstemmed | Morphological, physiological, and transcriptional responses to low nitrogen stress in Populus deltoides Marsh. clones with contrasting nitrogen use efficiency |
| title_short | Morphological, physiological, and transcriptional responses to low nitrogen stress in Populus deltoides Marsh. clones with contrasting nitrogen use efficiency |
| title_sort | morphological physiological and transcriptional responses to low nitrogen stress in populus deltoides marsh clones with contrasting nitrogen use efficiency |
| topic | Nitrogen deficiency Nitrogen use efficiency Gene expression Populus deltoides Marsh. |
| url | https://doi.org/10.1186/s12864-021-07991-7 |
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