Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.)
Abstract Background The SnRKs (sucrose non-fermenting 1 related protein kinase) are a gene family coding for Ser/Thr protein kinases and play important roles in linking the tolerance and metabolic responses of plants to abiotic stresses. To date, no genome-wide characterization of the sucrose non-fe...
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BMC
2017-05-01
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| Series: | BMC Genetics |
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| Online Access: | http://link.springer.com/article/10.1186/s12863-017-0506-6 |
| _version_ | 1818038415122235392 |
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| author | Jiangping Bai Juan Mao Hongyu Yang Awais Khan Aqi Fan Siyan Liu Junlian Zhang Di Wang Huijuan Gao Jinlin Zhang |
| author_facet | Jiangping Bai Juan Mao Hongyu Yang Awais Khan Aqi Fan Siyan Liu Junlian Zhang Di Wang Huijuan Gao Jinlin Zhang |
| author_sort | Jiangping Bai |
| collection | DOAJ |
| description | Abstract Background The SnRKs (sucrose non-fermenting 1 related protein kinase) are a gene family coding for Ser/Thr protein kinases and play important roles in linking the tolerance and metabolic responses of plants to abiotic stresses. To date, no genome-wide characterization of the sucrose non-ferment 1 related protein kinase 2 (SnRK2) subfamily has been conducted in potato (Solanum tuberosum L.). Results In this study, eight StSnRK2 genes (StSnRK2.1- StSnRK2.8) were identified in the genome of the potato (Solanum tuberosum L.) cultivar ‘Longshu 3’, with similar characteristics to SnRK2 from other plant species in gene structure, motif distribution and secondary structures. The C-terminal regions were highly divergent among StSnRK2s, while they all carried the similar Ser/Thr protein kinase domain. The fluorescence of GFP fused with StSnRK2.1, StSnRK2.2, StSnRK2.6, StSnRK2.7 and StSnRK2.8 was detected in the nucleus and cytoplasm of onion epidermal cells with StSnRK2.3 and StSnRK2.4 mainly associated to the nucleus while StSnRK2.5 to subcellular organelles. Expression level analysis by qRT-PCR showed that StSnRK2.1, 2.2, 2.5 and 2.6 were more than 1 fold higher in the root than in the leaf, tuber and stem tissues. The expressions of StSnRK2.3, 2.7, and 2.8 were at least 1.5 folds higher in the leaf and stem than in the root, but lower in the tuber. The expression of StSnRK2.4 was also significantly (P < 0.05) higher in leaf, stem, and tuber than in the root. From the perspective of the relative expressions of StSnRK2 genes in potato, ABA treatment had a different effect from NaCl and PEG treatments. Conclusion In the present study, we identified and characterized eight SnRK2s in the potato genome. The eight StSnRK2s exhibit similar gene structure and secondary structures in potato to the SnRK2s found in other plant species. The relative expression of eight genes varied among various tissues (roots, leaves, tubers, and stems) and abiotic stresses (ABA, NaCl and PEG-6000) with the prolongation of treatments. This study provides valuable information for the future functional dissection of potato SnRK2 genes in stress signal transduction, plant growth and development. |
| first_indexed | 2024-12-10T07:42:22Z |
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| id | doaj.art-7458a329cc324bfa804c81dbab1212f3 |
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| language | English |
| last_indexed | 2024-12-10T07:42:22Z |
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| spelling | doaj.art-7458a329cc324bfa804c81dbab1212f32022-12-22T01:57:16ZengBMCBMC Genetics1471-21562017-05-0118111510.1186/s12863-017-0506-6Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.)Jiangping Bai0Juan Mao1Hongyu Yang2Awais Khan3Aqi Fan4Siyan Liu5Junlian Zhang6Di Wang7Huijuan Gao8Jinlin Zhang9Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop ScienceGansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop ScienceGansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop ScienceInternational Potato Center (CIP)College of Agronomy, Gansu Agricultural UniversityCollege of Agronomy, Gansu Agricultural UniversityGansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop ScienceGansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop ScienceState Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou UniversityGansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop ScienceAbstract Background The SnRKs (sucrose non-fermenting 1 related protein kinase) are a gene family coding for Ser/Thr protein kinases and play important roles in linking the tolerance and metabolic responses of plants to abiotic stresses. To date, no genome-wide characterization of the sucrose non-ferment 1 related protein kinase 2 (SnRK2) subfamily has been conducted in potato (Solanum tuberosum L.). Results In this study, eight StSnRK2 genes (StSnRK2.1- StSnRK2.8) were identified in the genome of the potato (Solanum tuberosum L.) cultivar ‘Longshu 3’, with similar characteristics to SnRK2 from other plant species in gene structure, motif distribution and secondary structures. The C-terminal regions were highly divergent among StSnRK2s, while they all carried the similar Ser/Thr protein kinase domain. The fluorescence of GFP fused with StSnRK2.1, StSnRK2.2, StSnRK2.6, StSnRK2.7 and StSnRK2.8 was detected in the nucleus and cytoplasm of onion epidermal cells with StSnRK2.3 and StSnRK2.4 mainly associated to the nucleus while StSnRK2.5 to subcellular organelles. Expression level analysis by qRT-PCR showed that StSnRK2.1, 2.2, 2.5 and 2.6 were more than 1 fold higher in the root than in the leaf, tuber and stem tissues. The expressions of StSnRK2.3, 2.7, and 2.8 were at least 1.5 folds higher in the leaf and stem than in the root, but lower in the tuber. The expression of StSnRK2.4 was also significantly (P < 0.05) higher in leaf, stem, and tuber than in the root. From the perspective of the relative expressions of StSnRK2 genes in potato, ABA treatment had a different effect from NaCl and PEG treatments. Conclusion In the present study, we identified and characterized eight SnRK2s in the potato genome. The eight StSnRK2s exhibit similar gene structure and secondary structures in potato to the SnRK2s found in other plant species. The relative expression of eight genes varied among various tissues (roots, leaves, tubers, and stems) and abiotic stresses (ABA, NaCl and PEG-6000) with the prolongation of treatments. This study provides valuable information for the future functional dissection of potato SnRK2 genes in stress signal transduction, plant growth and development.http://link.springer.com/article/10.1186/s12863-017-0506-6PotatoStSnRK2Stress responsesABANaClPEG-6000 |
| spellingShingle | Jiangping Bai Juan Mao Hongyu Yang Awais Khan Aqi Fan Siyan Liu Junlian Zhang Di Wang Huijuan Gao Jinlin Zhang Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.) BMC Genetics Potato StSnRK2 Stress responses ABA NaCl PEG-6000 |
| title | Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.) |
| title_full | Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.) |
| title_fullStr | Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.) |
| title_full_unstemmed | Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.) |
| title_short | Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.) |
| title_sort | sucrose non ferment 1 related protein kinase 2 snrk2 genes could mediate the stress responses in potato solanum tuberosum l |
| topic | Potato StSnRK2 Stress responses ABA NaCl PEG-6000 |
| url | http://link.springer.com/article/10.1186/s12863-017-0506-6 |
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