The Effects of Sodium Acetate on the Immune Functions of Peripheral Mononuclear Cells and Polymorphonuclear Granulocytes in Postpartum Dairy Cows
Excessive lipid mobilization will snatch cell membrane lipids in postpartum dairy cows, which may impair the function of immune cells, including peripheral mononuclear cells (PBMCs) and polymorphonuclear granulocytes (PMNs). Acetate, as a precursor and the energy source of milk fat synthesis, plays...
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MDPI AG
2023-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/17/2721 |
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| author | Cong Yuan Dejin Tan Zitong Meng Maocheng Jiang Miao Lin Guoqi Zhao Kang Zhan |
| author_facet | Cong Yuan Dejin Tan Zitong Meng Maocheng Jiang Miao Lin Guoqi Zhao Kang Zhan |
| author_sort | Cong Yuan |
| collection | DOAJ |
| description | Excessive lipid mobilization will snatch cell membrane lipids in postpartum dairy cows, which may impair the function of immune cells, including peripheral mononuclear cells (PBMCs) and polymorphonuclear granulocytes (PMNs). Acetate, as a precursor and the energy source of milk fat synthesis, plays a key role in lipid synthesis and the energy supply of dairy cows. However, there is little information about the effect of sodium acetate (NaAc) on the immune function of PBMC and PMN in postpartum dairy cows. Therefore, this study aimed to evaluate the effects of NaAc on the immune functions of PBMCs and PMNs in postpartum dairy cows. In this experiment, twenty-four postpartum multiparous Holstein cows were randomly selected and divided into a NaAc treatment group and a control group. Our results demonstrated that the dietary addition of NaAc increased (<i>p</i> < 0.05) the number of monocytes and the monocyte ratio, suggesting that these postpartum cows fed with NaAc may have better immunity. These expressions of genes (<i>LAP</i>, <i>XBP1</i>, and <i>TAP</i>) involved in the antimicrobial activity in PBMCs were elevated (<i>p</i> < 0.05), suggesting that postpartum dairy cows supplemented with NaAc had the ability of antimicrobial activity. In addition, the mRNA expression of the monocarboxylate transporters <i>MCT1</i> and <i>MCT4</i> in PBMCs was increased (<i>p</i> < 0.05) in diets supplemented with NaAc in comparison to the control. Notably, the expression of the <i>XBP1</i> gene related to antimicrobial activity in PMN was upregulated with the addition of NaAc. The mRNA expression of genes (<i>TLN1</i>, <i>ITGB2</i>, and <i>SELL</i>) involved in adhesion was profoundly increased (<i>p</i> < 0.05) in the NaAc groups. In conclusion, our study provided a novel resolution strategy in which the use of NaAc can contribute to immunity in postpartum dairy cows by enhancing the ability of antimicrobial and adhesion in PBMCs and PMNs. |
| first_indexed | 2024-03-10T23:28:41Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T23:28:41Z |
| publishDate | 2023-08-01 |
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| spelling | doaj.art-745e6a535aaf426c878d887b5c3b15d32023-11-19T07:46:28ZengMDPI AGAnimals2076-26152023-08-011317272110.3390/ani13172721The Effects of Sodium Acetate on the Immune Functions of Peripheral Mononuclear Cells and Polymorphonuclear Granulocytes in Postpartum Dairy CowsCong Yuan0Dejin Tan1Zitong Meng2Maocheng Jiang3Miao Lin4Guoqi Zhao5Kang Zhan6Institute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaInstitute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaExcessive lipid mobilization will snatch cell membrane lipids in postpartum dairy cows, which may impair the function of immune cells, including peripheral mononuclear cells (PBMCs) and polymorphonuclear granulocytes (PMNs). Acetate, as a precursor and the energy source of milk fat synthesis, plays a key role in lipid synthesis and the energy supply of dairy cows. However, there is little information about the effect of sodium acetate (NaAc) on the immune function of PBMC and PMN in postpartum dairy cows. Therefore, this study aimed to evaluate the effects of NaAc on the immune functions of PBMCs and PMNs in postpartum dairy cows. In this experiment, twenty-four postpartum multiparous Holstein cows were randomly selected and divided into a NaAc treatment group and a control group. Our results demonstrated that the dietary addition of NaAc increased (<i>p</i> < 0.05) the number of monocytes and the monocyte ratio, suggesting that these postpartum cows fed with NaAc may have better immunity. These expressions of genes (<i>LAP</i>, <i>XBP1</i>, and <i>TAP</i>) involved in the antimicrobial activity in PBMCs were elevated (<i>p</i> < 0.05), suggesting that postpartum dairy cows supplemented with NaAc had the ability of antimicrobial activity. In addition, the mRNA expression of the monocarboxylate transporters <i>MCT1</i> and <i>MCT4</i> in PBMCs was increased (<i>p</i> < 0.05) in diets supplemented with NaAc in comparison to the control. Notably, the expression of the <i>XBP1</i> gene related to antimicrobial activity in PMN was upregulated with the addition of NaAc. The mRNA expression of genes (<i>TLN1</i>, <i>ITGB2</i>, and <i>SELL</i>) involved in adhesion was profoundly increased (<i>p</i> < 0.05) in the NaAc groups. In conclusion, our study provided a novel resolution strategy in which the use of NaAc can contribute to immunity in postpartum dairy cows by enhancing the ability of antimicrobial and adhesion in PBMCs and PMNs.https://www.mdpi.com/2076-2615/13/17/2721acetateperipheral blood mononuclear cellpolymorphonuclear granulocytepostpartum |
| spellingShingle | Cong Yuan Dejin Tan Zitong Meng Maocheng Jiang Miao Lin Guoqi Zhao Kang Zhan The Effects of Sodium Acetate on the Immune Functions of Peripheral Mononuclear Cells and Polymorphonuclear Granulocytes in Postpartum Dairy Cows Animals acetate peripheral blood mononuclear cell polymorphonuclear granulocyte postpartum |
| title | The Effects of Sodium Acetate on the Immune Functions of Peripheral Mononuclear Cells and Polymorphonuclear Granulocytes in Postpartum Dairy Cows |
| title_full | The Effects of Sodium Acetate on the Immune Functions of Peripheral Mononuclear Cells and Polymorphonuclear Granulocytes in Postpartum Dairy Cows |
| title_fullStr | The Effects of Sodium Acetate on the Immune Functions of Peripheral Mononuclear Cells and Polymorphonuclear Granulocytes in Postpartum Dairy Cows |
| title_full_unstemmed | The Effects of Sodium Acetate on the Immune Functions of Peripheral Mononuclear Cells and Polymorphonuclear Granulocytes in Postpartum Dairy Cows |
| title_short | The Effects of Sodium Acetate on the Immune Functions of Peripheral Mononuclear Cells and Polymorphonuclear Granulocytes in Postpartum Dairy Cows |
| title_sort | effects of sodium acetate on the immune functions of peripheral mononuclear cells and polymorphonuclear granulocytes in postpartum dairy cows |
| topic | acetate peripheral blood mononuclear cell polymorphonuclear granulocyte postpartum |
| url | https://www.mdpi.com/2076-2615/13/17/2721 |
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