Antimicrobial susceptibility profiles and coagulase gene polymorphism of staphylococcus aureus isolated from bovine subclinical mastitis
The purpose of the study was to isolate Staphylococcus aureus from bovine subclinical mastitis, determine their antibiotic susceptibilities and investigate the coagulase gene polymorphism by using a PCR-based restriction fragment length polymorphism (RFLP) method. Milk samples from 463 CMT positive...
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| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2017-07-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2081 |
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| author | Beytullah KENAR Arzu Funda BAGCIGIL Yahya KUYUCUOGLU Beren Başaran KAHRAMAN Selahattin KONAK |
| author_facet | Beytullah KENAR Arzu Funda BAGCIGIL Yahya KUYUCUOGLU Beren Başaran KAHRAMAN Selahattin KONAK |
| author_sort | Beytullah KENAR |
| collection | DOAJ |
| description | The purpose of the study was to isolate Staphylococcus aureus from bovine subclinical mastitis, determine their antibiotic susceptibilities and investigate the
coagulase gene polymorphism by using a PCR-based restriction fragment length polymorphism (RFLP) method. Milk samples from 463 CMT positive udders
from 237 cows cultured. The antimicrobial susceptibility of the isolates were determined by disc diffusion method. A total of 82 out of the 83 isolates (98.8%)
were found to be resistant at least one out of the 16 antibiotics studied. In this experiment 53 isolates (63.8%) were found to be resistant to penicillin; 52 (62.67%)
to trimethoprim/sulphamethoxazole; 51 (61.5%) to ampicillin; 40 (48.2%) to erytromycin; 29 (34.9%) to tetracycline; 18 (21.6%) to ciprofloxacin, 16 (19.3%) to
clindamycin, 13 (15.6%) to chloramphenicol; 8 (9.6%) to gentamicin; 5 (6.0%) to cefoxitin; 4 (4.9%) to vancomycin; 3 (3.6%) to cephalotin; 2 (2.4%) nafcillin; one
(1.2%) to oxacillin and one to (1.2%) furazolidon. No imipenem resistance was seen in the S. aureus isolates. The coagulase gen polymorphism were examined
by PCR amplification of coagulase gene followed by AluI digestion of repeating 81 bp DNA sequences. After nested PCR, double bands were produced in 8 of
the isolates while there were single band in remaining 75 isolates. Following AluI digestion, isolates that formed single band in length of approximately 300 bp
showed 3 different groups |
| first_indexed | 2024-03-13T04:37:24Z |
| format | Article |
| id | doaj.art-745e717f29c543e08b0308d9b3cd28d1 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:24Z |
| publishDate | 2017-07-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-745e717f29c543e08b0308d9b3cd28d12023-06-19T06:45:25ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512017-07-0123453554010.9775/kvfd.2016.172472081Antimicrobial susceptibility profiles and coagulase gene polymorphism of staphylococcus aureus isolated from bovine subclinical mastitisBeytullah KENAR0Arzu Funda BAGCIGIL1Yahya KUYUCUOGLU2Beren Başaran KAHRAMAN3Selahattin KONAK4Department of Microbiology, Faculty of Veterinary Medicine, Afyon Kocatepe University, TR-03200 Afyonkarahisar - TURKEYDepartment of Microbiology, Faculty of Veterinary Medicine, Istanbul University, TR-34320 Avcılar, Istanbul - TURKEYDepartment of Microbiology, Faculty of Veterinary Medicine, Afyon Kocatepe University, TR-03200 Afyonkarahisar - TURKEYDepartment of Microbiology, Faculty of Veterinary Medicine, Istanbul University, TR-34320 Avcılar, Istanbul - TURKEYDepartment Microbiology, Faculty of Veterinary Medicine, Afyon Kocatepe University, TR-03200 Afyonkarahisar - TURKEYThe purpose of the study was to isolate Staphylococcus aureus from bovine subclinical mastitis, determine their antibiotic susceptibilities and investigate the coagulase gene polymorphism by using a PCR-based restriction fragment length polymorphism (RFLP) method. Milk samples from 463 CMT positive udders from 237 cows cultured. The antimicrobial susceptibility of the isolates were determined by disc diffusion method. A total of 82 out of the 83 isolates (98.8%) were found to be resistant at least one out of the 16 antibiotics studied. In this experiment 53 isolates (63.8%) were found to be resistant to penicillin; 52 (62.67%) to trimethoprim/sulphamethoxazole; 51 (61.5%) to ampicillin; 40 (48.2%) to erytromycin; 29 (34.9%) to tetracycline; 18 (21.6%) to ciprofloxacin, 16 (19.3%) to clindamycin, 13 (15.6%) to chloramphenicol; 8 (9.6%) to gentamicin; 5 (6.0%) to cefoxitin; 4 (4.9%) to vancomycin; 3 (3.6%) to cephalotin; 2 (2.4%) nafcillin; one (1.2%) to oxacillin and one to (1.2%) furazolidon. No imipenem resistance was seen in the S. aureus isolates. The coagulase gen polymorphism were examined by PCR amplification of coagulase gene followed by AluI digestion of repeating 81 bp DNA sequences. After nested PCR, double bands were produced in 8 of the isolates while there were single band in remaining 75 isolates. Following AluI digestion, isolates that formed single band in length of approximately 300 bp showed 3 different groupshttps://vetdergikafkas.org/pdf.php?id=2081bovine subclinical mastitisstaphylococcus aureusantibiotic susceptibilitycoagulase gene polymorphism |
| spellingShingle | Beytullah KENAR Arzu Funda BAGCIGIL Yahya KUYUCUOGLU Beren Başaran KAHRAMAN Selahattin KONAK Antimicrobial susceptibility profiles and coagulase gene polymorphism of staphylococcus aureus isolated from bovine subclinical mastitis Kafkas Universitesi Veteriner Fakültesi Dergisi bovine subclinical mastitis staphylococcus aureus antibiotic susceptibility coagulase gene polymorphism |
| title | Antimicrobial susceptibility profiles and coagulase gene polymorphism of staphylococcus aureus isolated from bovine subclinical mastitis |
| title_full | Antimicrobial susceptibility profiles and coagulase gene polymorphism of staphylococcus aureus isolated from bovine subclinical mastitis |
| title_fullStr | Antimicrobial susceptibility profiles and coagulase gene polymorphism of staphylococcus aureus isolated from bovine subclinical mastitis |
| title_full_unstemmed | Antimicrobial susceptibility profiles and coagulase gene polymorphism of staphylococcus aureus isolated from bovine subclinical mastitis |
| title_short | Antimicrobial susceptibility profiles and coagulase gene polymorphism of staphylococcus aureus isolated from bovine subclinical mastitis |
| title_sort | antimicrobial susceptibility profiles and coagulase gene polymorphism of staphylococcus aureus isolated from bovine subclinical mastitis |
| topic | bovine subclinical mastitis staphylococcus aureus antibiotic susceptibility coagulase gene polymorphism |
| url | https://vetdergikafkas.org/pdf.php?id=2081 |
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