Live cell imaging of single RNA molecules with fluorogenic Mango II arrays
Fluorogenic RNA aptamers have been used for RNA imaging, but folding and fluorescence stability often limited their use in high resolution applications. Here the authors present an array of stably folding Mango II aptamers for imaging of coding and non-coding RNAs at single-molecule resolution, in b...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-14932-7 |
| _version_ | 1818856007994966016 |
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| author | Adam D. Cawte Peter J. Unrau David S. Rueda |
| author_facet | Adam D. Cawte Peter J. Unrau David S. Rueda |
| author_sort | Adam D. Cawte |
| collection | DOAJ |
| description | Fluorogenic RNA aptamers have been used for RNA imaging, but folding and fluorescence stability often limited their use in high resolution applications. Here the authors present an array of stably folding Mango II aptamers for imaging of coding and non-coding RNAs at single-molecule resolution, in both live and fixed cells. |
| first_indexed | 2024-12-19T08:17:40Z |
| format | Article |
| id | doaj.art-7460f7c8bd624b6fa2e3fe48d7d0f593 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T08:17:40Z |
| publishDate | 2020-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-7460f7c8bd624b6fa2e3fe48d7d0f5932022-12-21T20:29:27ZengNature PortfolioNature Communications2041-17232020-03-0111111110.1038/s41467-020-14932-7Live cell imaging of single RNA molecules with fluorogenic Mango II arraysAdam D. Cawte0Peter J. Unrau1David S. Rueda2Single Molecule Imaging Group, MRC London Institute of Medical SciencesDepartment of Molecular Biology and Biochemistry, Simon Fraser UniversitySingle Molecule Imaging Group, MRC London Institute of Medical SciencesFluorogenic RNA aptamers have been used for RNA imaging, but folding and fluorescence stability often limited their use in high resolution applications. Here the authors present an array of stably folding Mango II aptamers for imaging of coding and non-coding RNAs at single-molecule resolution, in both live and fixed cells.https://doi.org/10.1038/s41467-020-14932-7 |
| spellingShingle | Adam D. Cawte Peter J. Unrau David S. Rueda Live cell imaging of single RNA molecules with fluorogenic Mango II arrays Nature Communications |
| title | Live cell imaging of single RNA molecules with fluorogenic Mango II arrays |
| title_full | Live cell imaging of single RNA molecules with fluorogenic Mango II arrays |
| title_fullStr | Live cell imaging of single RNA molecules with fluorogenic Mango II arrays |
| title_full_unstemmed | Live cell imaging of single RNA molecules with fluorogenic Mango II arrays |
| title_short | Live cell imaging of single RNA molecules with fluorogenic Mango II arrays |
| title_sort | live cell imaging of single rna molecules with fluorogenic mango ii arrays |
| url | https://doi.org/10.1038/s41467-020-14932-7 |
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