The function of BoTCP25 in the regulation of leaf development of Chinese kale
XG Chinese kale (Brassica oleracea cv. ‘XiangGu’) is a variety of Chinese kale and has metamorphic leaves attached to the true leaves. Metamorphic leaves are secondary leaves emerging from the veins of true leaves. However, it remains unknown how the formation of metamorphic leaves is regulated and...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1127197/full |
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| author | Jiajing Zeng Mengyu Yang Jing Deng Dongyang Zheng Zhongxiong Lai Gefu Wang-Pruski Gefu Wang-Pruski Xu XuHan Xu XuHan Rongfang Guo |
| author_facet | Jiajing Zeng Mengyu Yang Jing Deng Dongyang Zheng Zhongxiong Lai Gefu Wang-Pruski Gefu Wang-Pruski Xu XuHan Xu XuHan Rongfang Guo |
| author_sort | Jiajing Zeng |
| collection | DOAJ |
| description | XG Chinese kale (Brassica oleracea cv. ‘XiangGu’) is a variety of Chinese kale and has metamorphic leaves attached to the true leaves. Metamorphic leaves are secondary leaves emerging from the veins of true leaves. However, it remains unknown how the formation of metamorphic leaves is regulated and whether it differs from normal leaves. BoTCP25 is differentially expressed in different parts of XG leaves and respond to auxin signals. To clarify the function of BoTCP25 in XG Chinese kale leaves, we overexpressed BoTCP25 in XG and Arabidopsis, and interestingly, its overexpression caused Chinese kale leaves to curl and changed the location of metamorphic leaves, whereas heterologous expression of BoTCP25 in Arabidopsis did not show metamorphic leaves, but only an increase in leaf number and leaf area. Further analysis of the expression of related genes in Chinese kale and Arabidopsis overexpressing BoTCP25 revealed that BoTCP25 could directly bind the promoter of BoNGA3, a transcription factor related to leaf development, and induce a significant expression of BoNGA3 in transgenic Chinese kale plants, whereas this induction of NGA3 did not occur in transgenic Arabidopsis. This suggests that the regulation of Chinese kale metamorphic leaves by BoTCP25 is dependent on a regulatory pathway or elements specific to XG and that this regulatory element may be repressed or absent from Arabidopsis. In addition, the expression of miR319’s precursor, a negative regulator of BoTCP25, also differed in transgenic Chinese kale and Arabidopsis. miR319’s transcrips were significantly up-regulated in transgenic Chinese kale mature leaves, while in transgenic Arabidopsis, the expression of miR319 in mature leaves was kept low. In conclusion, the differential expression of BoNGA3 and miR319 in the two species may be related to the exertion of BoTCP25 function, thus partially contributing to the differences in leaf phenotypes between overexpressed BoTCP25 in Arabidopsis and Chinese kale. |
| first_indexed | 2024-04-09T17:31:29Z |
| format | Article |
| id | doaj.art-7466559e45824fb4be0257111ef653d7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T17:31:29Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-7466559e45824fb4be0257111ef653d72023-04-18T05:21:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-04-011410.3389/fpls.2023.11271971127197The function of BoTCP25 in the regulation of leaf development of Chinese kaleJiajing Zeng0Mengyu Yang1Jing Deng2Dongyang Zheng3Zhongxiong Lai4Gefu Wang-Pruski5Gefu Wang-Pruski6Xu XuHan7Xu XuHan8Rongfang Guo9College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, ChinaDepartment of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS, CanadaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, ChinaFaculté des sciences et de la technologie, Institut de la Recherche Interdiciplinaire de Toulouse (IRIT-ARI), Toulouse, FranceCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, ChinaXG Chinese kale (Brassica oleracea cv. ‘XiangGu’) is a variety of Chinese kale and has metamorphic leaves attached to the true leaves. Metamorphic leaves are secondary leaves emerging from the veins of true leaves. However, it remains unknown how the formation of metamorphic leaves is regulated and whether it differs from normal leaves. BoTCP25 is differentially expressed in different parts of XG leaves and respond to auxin signals. To clarify the function of BoTCP25 in XG Chinese kale leaves, we overexpressed BoTCP25 in XG and Arabidopsis, and interestingly, its overexpression caused Chinese kale leaves to curl and changed the location of metamorphic leaves, whereas heterologous expression of BoTCP25 in Arabidopsis did not show metamorphic leaves, but only an increase in leaf number and leaf area. Further analysis of the expression of related genes in Chinese kale and Arabidopsis overexpressing BoTCP25 revealed that BoTCP25 could directly bind the promoter of BoNGA3, a transcription factor related to leaf development, and induce a significant expression of BoNGA3 in transgenic Chinese kale plants, whereas this induction of NGA3 did not occur in transgenic Arabidopsis. This suggests that the regulation of Chinese kale metamorphic leaves by BoTCP25 is dependent on a regulatory pathway or elements specific to XG and that this regulatory element may be repressed or absent from Arabidopsis. In addition, the expression of miR319’s precursor, a negative regulator of BoTCP25, also differed in transgenic Chinese kale and Arabidopsis. miR319’s transcrips were significantly up-regulated in transgenic Chinese kale mature leaves, while in transgenic Arabidopsis, the expression of miR319 in mature leaves was kept low. In conclusion, the differential expression of BoNGA3 and miR319 in the two species may be related to the exertion of BoTCP25 function, thus partially contributing to the differences in leaf phenotypes between overexpressed BoTCP25 in Arabidopsis and Chinese kale.https://www.frontiersin.org/articles/10.3389/fpls.2023.1127197/fullBoTCP25Chinese kaleleafBoNGA3metamorphic leaf |
| spellingShingle | Jiajing Zeng Mengyu Yang Jing Deng Dongyang Zheng Zhongxiong Lai Gefu Wang-Pruski Gefu Wang-Pruski Xu XuHan Xu XuHan Rongfang Guo The function of BoTCP25 in the regulation of leaf development of Chinese kale Frontiers in Plant Science BoTCP25 Chinese kale leaf BoNGA3 metamorphic leaf |
| title | The function of BoTCP25 in the regulation of leaf development of Chinese kale |
| title_full | The function of BoTCP25 in the regulation of leaf development of Chinese kale |
| title_fullStr | The function of BoTCP25 in the regulation of leaf development of Chinese kale |
| title_full_unstemmed | The function of BoTCP25 in the regulation of leaf development of Chinese kale |
| title_short | The function of BoTCP25 in the regulation of leaf development of Chinese kale |
| title_sort | function of botcp25 in the regulation of leaf development of chinese kale |
| topic | BoTCP25 Chinese kale leaf BoNGA3 metamorphic leaf |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1127197/full |
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