Investigation of cellular and molecular responses to pulsed focused ultrasound in a mouse model.
Continuous focused ultrasound (cFUS) has been widely used for thermal ablation of tissues, relying on continuous exposures to generate temperatures necessary to induce coagulative necrosis. Pulsed FUS (pFUS) employs non-continuous exposures that lower the rate of energy deposition and allow cooling...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3172304?pdf=render |
| _version_ | 1811210510400487424 |
|---|---|
| author | Scott R Burks Ali Ziadloo Hilary A Hancock Aneeka Chaudhry Dana D Dean Bobbi K Lewis Victor Frenkel Joseph A Frank |
| author_facet | Scott R Burks Ali Ziadloo Hilary A Hancock Aneeka Chaudhry Dana D Dean Bobbi K Lewis Victor Frenkel Joseph A Frank |
| author_sort | Scott R Burks |
| collection | DOAJ |
| description | Continuous focused ultrasound (cFUS) has been widely used for thermal ablation of tissues, relying on continuous exposures to generate temperatures necessary to induce coagulative necrosis. Pulsed FUS (pFUS) employs non-continuous exposures that lower the rate of energy deposition and allow cooling to occur between pulses, thereby minimizing thermal effects and emphasizing effects created by non-thermal mechanisms of FUS (i.e., acoustic radiation forces and acoustic cavitation). pFUS has shown promise for a variety of applications including drug and nanoparticle delivery; however, little is understood about the effects these exposures have on tissue, especially with regard to cellular pro-homing factors (growth factors, cytokines, and cell adhesion molecules). We examined changes in murine hamstring muscle following pFUS or cFUS and demonstrate that pFUS, unlike cFUS, has little effect on the histological integrity of muscle and does not induce cell death. Infiltration of macrophages was observed 3 and 8 days following pFUS or cFUS exposures. pFUS increased expression of several cytokines (e.g., IL-1α, IL-1β, TNFα, INFγ, MIP-1α, MCP-1, and GMCSF) creating a local cytokine gradient on days 0 and 1 post-pFUS that returns to baseline levels by day 3 post-pFUS. pFUS exposures induced upregulation of other signaling molecules (e.g., VEGF, FGF, PlGF, HGF, and SDF-1α) and cell adhesion molecules (e.g., ICAM-1 and VCAM-1) on muscle vasculature. The observed molecular changes in muscle following pFUS may be utilized to target cellular therapies by increasing homing to areas of pathology. |
| first_indexed | 2024-04-12T04:56:54Z |
| format | Article |
| id | doaj.art-74671b6990ad4cd9a555bbd6e46f1f63 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:56:54Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-74671b6990ad4cd9a555bbd6e46f1f632022-12-22T03:47:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2473010.1371/journal.pone.0024730Investigation of cellular and molecular responses to pulsed focused ultrasound in a mouse model.Scott R BurksAli ZiadlooHilary A HancockAneeka ChaudhryDana D DeanBobbi K LewisVictor FrenkelJoseph A FrankContinuous focused ultrasound (cFUS) has been widely used for thermal ablation of tissues, relying on continuous exposures to generate temperatures necessary to induce coagulative necrosis. Pulsed FUS (pFUS) employs non-continuous exposures that lower the rate of energy deposition and allow cooling to occur between pulses, thereby minimizing thermal effects and emphasizing effects created by non-thermal mechanisms of FUS (i.e., acoustic radiation forces and acoustic cavitation). pFUS has shown promise for a variety of applications including drug and nanoparticle delivery; however, little is understood about the effects these exposures have on tissue, especially with regard to cellular pro-homing factors (growth factors, cytokines, and cell adhesion molecules). We examined changes in murine hamstring muscle following pFUS or cFUS and demonstrate that pFUS, unlike cFUS, has little effect on the histological integrity of muscle and does not induce cell death. Infiltration of macrophages was observed 3 and 8 days following pFUS or cFUS exposures. pFUS increased expression of several cytokines (e.g., IL-1α, IL-1β, TNFα, INFγ, MIP-1α, MCP-1, and GMCSF) creating a local cytokine gradient on days 0 and 1 post-pFUS that returns to baseline levels by day 3 post-pFUS. pFUS exposures induced upregulation of other signaling molecules (e.g., VEGF, FGF, PlGF, HGF, and SDF-1α) and cell adhesion molecules (e.g., ICAM-1 and VCAM-1) on muscle vasculature. The observed molecular changes in muscle following pFUS may be utilized to target cellular therapies by increasing homing to areas of pathology.http://europepmc.org/articles/PMC3172304?pdf=render |
| spellingShingle | Scott R Burks Ali Ziadloo Hilary A Hancock Aneeka Chaudhry Dana D Dean Bobbi K Lewis Victor Frenkel Joseph A Frank Investigation of cellular and molecular responses to pulsed focused ultrasound in a mouse model. PLoS ONE |
| title | Investigation of cellular and molecular responses to pulsed focused ultrasound in a mouse model. |
| title_full | Investigation of cellular and molecular responses to pulsed focused ultrasound in a mouse model. |
| title_fullStr | Investigation of cellular and molecular responses to pulsed focused ultrasound in a mouse model. |
| title_full_unstemmed | Investigation of cellular and molecular responses to pulsed focused ultrasound in a mouse model. |
| title_short | Investigation of cellular and molecular responses to pulsed focused ultrasound in a mouse model. |
| title_sort | investigation of cellular and molecular responses to pulsed focused ultrasound in a mouse model |
| url | http://europepmc.org/articles/PMC3172304?pdf=render |
| work_keys_str_mv | AT scottrburks investigationofcellularandmolecularresponsestopulsedfocusedultrasoundinamousemodel AT aliziadloo investigationofcellularandmolecularresponsestopulsedfocusedultrasoundinamousemodel AT hilaryahancock investigationofcellularandmolecularresponsestopulsedfocusedultrasoundinamousemodel AT aneekachaudhry investigationofcellularandmolecularresponsestopulsedfocusedultrasoundinamousemodel AT danaddean investigationofcellularandmolecularresponsestopulsedfocusedultrasoundinamousemodel AT bobbiklewis investigationofcellularandmolecularresponsestopulsedfocusedultrasoundinamousemodel AT victorfrenkel investigationofcellularandmolecularresponsestopulsedfocusedultrasoundinamousemodel AT josephafrank investigationofcellularandmolecularresponsestopulsedfocusedultrasoundinamousemodel |