Topical anti-inflammatory and anti-oxidative effects of porcine placenta extracts on 2,4-dinitrochlorobenzene-induced contact dermatitis
Abstract Background The placenta is a reservoir enriched with growth factors, hormones, cytokines and minerals. While several beneficial effects of placenta extracts on wound healing, anti-aging and anti-inflammatory responses have been reported, relatively limited mechanistic exploration has been c...
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2396-1 |
| _version_ | 1818132597077704704 |
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| author | Jae Hyeok Heo Yoonki Heo Hee Jung Lee Minjee Kim Ha Youn Shin |
| author_facet | Jae Hyeok Heo Yoonki Heo Hee Jung Lee Minjee Kim Ha Youn Shin |
| author_sort | Jae Hyeok Heo |
| collection | DOAJ |
| description | Abstract Background The placenta is a reservoir enriched with growth factors, hormones, cytokines and minerals. While several beneficial effects of placenta extracts on wound healing, anti-aging and anti-inflammatory responses have been reported, relatively limited mechanistic exploration has been conducted to date. Here, we provide compelling evidence of anti-inflammatory and anti-oxidative activities of porcine placenta extracts (PPE) against contact dermatitis in vivo. Methods A contact dermatitis mouse model was established by sensitizing the dorsal skin of BALB/c mice using the contact allergen, 2,4-dinitrochlorobenzene (DNCB), and molecular consequences of topical application of PPE were investigated. PPEs were pre-sterilized via γ-irradiation, which is a milder but more effective way of sterilizing biomolecules relative to the conventional autoclaving method. Results DNCB-induced skin lesions displayed clear contact dermatitis-like symptoms and topical application of PPE dramatically alleviated both local and systemic inflammatory responses. Inflammatory epidermal thickening was completely abrogated and allergen-specific serum IgE levels significantly reduced in the presence of PPE. Moreover, anti-oxidative activities of PPE were observed both in vitro and in vivo, which may lead to attenuation of inflammatory responses. Prolonged treatment with PPE strongly inhibited production of DNCB-induced reactive oxygen species (ROS) and subsequently prevented oxidative degradation of hyaluronic acid (HA), which triggers innate inflammatory responses. Conclusion Our findings supply valuable insights into the mechanisms underlying the anti-inflammatory effects of PPE and provide a functional basis for the clinical application of PPE in inflammatory diseases. |
| first_indexed | 2024-12-11T08:39:21Z |
| format | Article |
| id | doaj.art-7467871623c04d23a294498f9646b846 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-11T08:39:21Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-7467871623c04d23a294498f9646b8462022-12-22T01:14:17ZengBMCBMC Complementary and Alternative Medicine1472-68822018-12-011811910.1186/s12906-018-2396-1Topical anti-inflammatory and anti-oxidative effects of porcine placenta extracts on 2,4-dinitrochlorobenzene-induced contact dermatitisJae Hyeok Heo0Yoonki Heo1Hee Jung Lee2Minjee Kim3Ha Youn Shin4Department of Biomedical Science and Engineering, Konkuk Institute of Technology, Konkuk UniversityDepartment of Biomedical Science and Engineering, Konkuk Institute of Technology, Konkuk UniversityDepartment of Biomedical Science and Engineering, Konkuk Institute of Technology, Konkuk UniversityDepartment of Biomedical Science and Engineering, Konkuk Institute of Technology, Konkuk UniversityDepartment of Biomedical Science and Engineering, Konkuk Institute of Technology, Konkuk UniversityAbstract Background The placenta is a reservoir enriched with growth factors, hormones, cytokines and minerals. While several beneficial effects of placenta extracts on wound healing, anti-aging and anti-inflammatory responses have been reported, relatively limited mechanistic exploration has been conducted to date. Here, we provide compelling evidence of anti-inflammatory and anti-oxidative activities of porcine placenta extracts (PPE) against contact dermatitis in vivo. Methods A contact dermatitis mouse model was established by sensitizing the dorsal skin of BALB/c mice using the contact allergen, 2,4-dinitrochlorobenzene (DNCB), and molecular consequences of topical application of PPE were investigated. PPEs were pre-sterilized via γ-irradiation, which is a milder but more effective way of sterilizing biomolecules relative to the conventional autoclaving method. Results DNCB-induced skin lesions displayed clear contact dermatitis-like symptoms and topical application of PPE dramatically alleviated both local and systemic inflammatory responses. Inflammatory epidermal thickening was completely abrogated and allergen-specific serum IgE levels significantly reduced in the presence of PPE. Moreover, anti-oxidative activities of PPE were observed both in vitro and in vivo, which may lead to attenuation of inflammatory responses. Prolonged treatment with PPE strongly inhibited production of DNCB-induced reactive oxygen species (ROS) and subsequently prevented oxidative degradation of hyaluronic acid (HA), which triggers innate inflammatory responses. Conclusion Our findings supply valuable insights into the mechanisms underlying the anti-inflammatory effects of PPE and provide a functional basis for the clinical application of PPE in inflammatory diseases.http://link.springer.com/article/10.1186/s12906-018-2396-1Placenta extractContact dermatitisDNCBROSHyaluronic acidAnti-inflammatory effects |
| spellingShingle | Jae Hyeok Heo Yoonki Heo Hee Jung Lee Minjee Kim Ha Youn Shin Topical anti-inflammatory and anti-oxidative effects of porcine placenta extracts on 2,4-dinitrochlorobenzene-induced contact dermatitis BMC Complementary and Alternative Medicine Placenta extract Contact dermatitis DNCB ROS Hyaluronic acid Anti-inflammatory effects |
| title | Topical anti-inflammatory and anti-oxidative effects of porcine placenta extracts on 2,4-dinitrochlorobenzene-induced contact dermatitis |
| title_full | Topical anti-inflammatory and anti-oxidative effects of porcine placenta extracts on 2,4-dinitrochlorobenzene-induced contact dermatitis |
| title_fullStr | Topical anti-inflammatory and anti-oxidative effects of porcine placenta extracts on 2,4-dinitrochlorobenzene-induced contact dermatitis |
| title_full_unstemmed | Topical anti-inflammatory and anti-oxidative effects of porcine placenta extracts on 2,4-dinitrochlorobenzene-induced contact dermatitis |
| title_short | Topical anti-inflammatory and anti-oxidative effects of porcine placenta extracts on 2,4-dinitrochlorobenzene-induced contact dermatitis |
| title_sort | topical anti inflammatory and anti oxidative effects of porcine placenta extracts on 2 4 dinitrochlorobenzene induced contact dermatitis |
| topic | Placenta extract Contact dermatitis DNCB ROS Hyaluronic acid Anti-inflammatory effects |
| url | http://link.springer.com/article/10.1186/s12906-018-2396-1 |
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