RNAi screen reveals potentially novel roles of cytokines in myoblast differentiation.
Cytokines are cell-secreted signaling molecules that modulate various cellular functions, with the best-characterized roles in immune responses. The expression of numerous cytokines in skeletal muscle tissues and muscle cells has been reported, but their function in skeletal myogenesis, the formatio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3699544?pdf=render |
| _version_ | 1818934975261573120 |
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| author | Yejing Ge Rachel J Waldemer Ramakrishna Nalluri Paul D Nuzzi Jie Chen |
| author_facet | Yejing Ge Rachel J Waldemer Ramakrishna Nalluri Paul D Nuzzi Jie Chen |
| author_sort | Yejing Ge |
| collection | DOAJ |
| description | Cytokines are cell-secreted signaling molecules that modulate various cellular functions, with the best-characterized roles in immune responses. The expression of numerous cytokines in skeletal muscle tissues and muscle cells has been reported, but their function in skeletal myogenesis, the formation of skeletal muscle, has been largely underexplored. To systematically examine the potential roles of cytokines in skeletal myogenesis, we undertook an RNAi screen of 134 mouse cytokine genes for their involvement in the differentiation of C2C12 myoblasts. Our results have uncovered 29 cytokines as strong candidates for novel myogenic regulators, potentially conferring positive and negative regulation at distinct stages of myogenesis. These candidates represent a diverse collection of cytokine families, including interleukins, TNF-related factors, and chemokines. Our findings suggest the fundamental importance of cytokines in the cell-autonomous regulation of myoblast differentiation, and may facilitate future identification of novel therapeutic targets for improving muscle regeneration and growth in health and diseases. |
| first_indexed | 2024-12-20T05:12:49Z |
| format | Article |
| id | doaj.art-746c506b561a48308ded5fe63d491d50 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T05:12:49Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-746c506b561a48308ded5fe63d491d502022-12-21T19:52:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6806810.1371/journal.pone.0068068RNAi screen reveals potentially novel roles of cytokines in myoblast differentiation.Yejing GeRachel J WaldemerRamakrishna NalluriPaul D NuzziJie ChenCytokines are cell-secreted signaling molecules that modulate various cellular functions, with the best-characterized roles in immune responses. The expression of numerous cytokines in skeletal muscle tissues and muscle cells has been reported, but their function in skeletal myogenesis, the formation of skeletal muscle, has been largely underexplored. To systematically examine the potential roles of cytokines in skeletal myogenesis, we undertook an RNAi screen of 134 mouse cytokine genes for their involvement in the differentiation of C2C12 myoblasts. Our results have uncovered 29 cytokines as strong candidates for novel myogenic regulators, potentially conferring positive and negative regulation at distinct stages of myogenesis. These candidates represent a diverse collection of cytokine families, including interleukins, TNF-related factors, and chemokines. Our findings suggest the fundamental importance of cytokines in the cell-autonomous regulation of myoblast differentiation, and may facilitate future identification of novel therapeutic targets for improving muscle regeneration and growth in health and diseases.http://europepmc.org/articles/PMC3699544?pdf=render |
| spellingShingle | Yejing Ge Rachel J Waldemer Ramakrishna Nalluri Paul D Nuzzi Jie Chen RNAi screen reveals potentially novel roles of cytokines in myoblast differentiation. PLoS ONE |
| title | RNAi screen reveals potentially novel roles of cytokines in myoblast differentiation. |
| title_full | RNAi screen reveals potentially novel roles of cytokines in myoblast differentiation. |
| title_fullStr | RNAi screen reveals potentially novel roles of cytokines in myoblast differentiation. |
| title_full_unstemmed | RNAi screen reveals potentially novel roles of cytokines in myoblast differentiation. |
| title_short | RNAi screen reveals potentially novel roles of cytokines in myoblast differentiation. |
| title_sort | rnai screen reveals potentially novel roles of cytokines in myoblast differentiation |
| url | http://europepmc.org/articles/PMC3699544?pdf=render |
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