Influence of concentration, time and method of application of citric acid and sodium citrate in root conditioning

OBJECTIVES: The aim of this study was to establish the parameters of concentration, time and mode of application of citric acid and sodium citrate in relation to root conditioning. MATERIAL AND METHODS: A total of 495 samples were obtained and equally distributed among 11 groups (5 for testing diffe...

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Main Authors: Rodrigo Cavassim, Fábio Renato Manzolli Leite, Daniela Leal Zandim, Andrea Abi Rached Dantas, Ricardo Samih Georges Abi Rached, José Eduardo Cezar Sampaio
Format: Article
Language:English
Published: University of São Paulo 2012-06-01
Series:Journal of Applied Oral Science
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572012000300014
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author Rodrigo Cavassim
Fábio Renato Manzolli Leite
Daniela Leal Zandim
Andrea Abi Rached Dantas
Ricardo Samih Georges Abi Rached
José Eduardo Cezar Sampaio
author_facet Rodrigo Cavassim
Fábio Renato Manzolli Leite
Daniela Leal Zandim
Andrea Abi Rached Dantas
Ricardo Samih Georges Abi Rached
José Eduardo Cezar Sampaio
author_sort Rodrigo Cavassim
collection DOAJ
description OBJECTIVES: The aim of this study was to establish the parameters of concentration, time and mode of application of citric acid and sodium citrate in relation to root conditioning. MATERIAL AND METHODS: A total of 495 samples were obtained and equally distributed among 11 groups (5 for testing different concentrations of citric acid, 5 for testing different concentrations of sodium citrate and 1 control group). After laboratorial processing, the samples were analyzed under scanning electron microscopy. A previously calibrated and blind examiner evaluated micrographs of the samples. Non-parametric statistical analysis was performed to analyze the data obtained. RESULTS: Brushing 25% citric acid for 3 min, promoted greater exposure of collagen fibers in comparison with the brushing of 1% citric acid for 1 minute and its topical application at 1% for 3 min. Sodium citrate exposed collagen fibers in a few number of samples. CONCLUSION: Despite the lack of statistical significance, better results for collagen exposure were obtained with brushing application of 25% citric acid for 3 min than with other application parameter. Sodium citrate produced a few number of samples with collagen exposure, so it is not indicated for root conditioning.
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spelling doaj.art-746c79ef57e54ce49068e0ecd2739fff2022-12-21T22:48:29ZengUniversity of São PauloJournal of Applied Oral Science1678-77571678-77652012-06-0120337638310.1590/S1678-77572012000300014Influence of concentration, time and method of application of citric acid and sodium citrate in root conditioningRodrigo CavassimFábio Renato Manzolli LeiteDaniela Leal ZandimAndrea Abi Rached DantasRicardo Samih Georges Abi RachedJosé Eduardo Cezar SampaioOBJECTIVES: The aim of this study was to establish the parameters of concentration, time and mode of application of citric acid and sodium citrate in relation to root conditioning. MATERIAL AND METHODS: A total of 495 samples were obtained and equally distributed among 11 groups (5 for testing different concentrations of citric acid, 5 for testing different concentrations of sodium citrate and 1 control group). After laboratorial processing, the samples were analyzed under scanning electron microscopy. A previously calibrated and blind examiner evaluated micrographs of the samples. Non-parametric statistical analysis was performed to analyze the data obtained. RESULTS: Brushing 25% citric acid for 3 min, promoted greater exposure of collagen fibers in comparison with the brushing of 1% citric acid for 1 minute and its topical application at 1% for 3 min. Sodium citrate exposed collagen fibers in a few number of samples. CONCLUSION: Despite the lack of statistical significance, better results for collagen exposure were obtained with brushing application of 25% citric acid for 3 min than with other application parameter. Sodium citrate produced a few number of samples with collagen exposure, so it is not indicated for root conditioning.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572012000300014PeriodonticsDental scalingSmear layerCollagen
spellingShingle Rodrigo Cavassim
Fábio Renato Manzolli Leite
Daniela Leal Zandim
Andrea Abi Rached Dantas
Ricardo Samih Georges Abi Rached
José Eduardo Cezar Sampaio
Influence of concentration, time and method of application of citric acid and sodium citrate in root conditioning
Journal of Applied Oral Science
Periodontics
Dental scaling
Smear layer
Collagen
title Influence of concentration, time and method of application of citric acid and sodium citrate in root conditioning
title_full Influence of concentration, time and method of application of citric acid and sodium citrate in root conditioning
title_fullStr Influence of concentration, time and method of application of citric acid and sodium citrate in root conditioning
title_full_unstemmed Influence of concentration, time and method of application of citric acid and sodium citrate in root conditioning
title_short Influence of concentration, time and method of application of citric acid and sodium citrate in root conditioning
title_sort influence of concentration time and method of application of citric acid and sodium citrate in root conditioning
topic Periodontics
Dental scaling
Smear layer
Collagen
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572012000300014
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