The effects of high-tannin leaf litter from transgenic poplars on microbial communities in microcosm soils.
The impacts of leaf litter from genetically-modified hybrid poplar accumulating high levels of condensed tannins (proanthocyanidins) were examined in soil microcosms consisting of moss growing on sieved soil. Moss preferentially proliferated in microcosms with lower tannin content; DGGE detected in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00290/full |
| _version_ | 1819130071805329408 |
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| author | Richard S. Winder Josyanne eLamarche C. Peter eConstabel Richard eHamelin |
| author_facet | Richard S. Winder Josyanne eLamarche C. Peter eConstabel Richard eHamelin |
| author_sort | Richard S. Winder |
| collection | DOAJ |
| description | The impacts of leaf litter from genetically-modified hybrid poplar accumulating high levels of condensed tannins (proanthocyanidins) were examined in soil microcosms consisting of moss growing on sieved soil. Moss preferentially proliferated in microcosms with lower tannin content; DGGE detected increased fungal diversity in microcosms with low-tannin litter. The proportion of cloned rDNA sequences from Actinobacteria decreased with litter addition while Bacteroidetes, Chloroflexi, Cyanobacteria, and α-Proteobacteria significantly increased. β–Proteobacteria were proportionally more numerous at high tannin levels. Tannins had no significant impact on overall diversity of bacterial communities analyzed with various estimators. There was an increased proportion of N-fixing bacteria corresponding to the addition of litter with low tannin levels. The addition of litter increased the proportion of Ascomycota/Basidiomycota. Dothideomycetes, Pucciniomycetes, and Tremellomycetes also increased and Agaricomycetes decreased. Agaricomycetes and Sordariomycetes were significantly more abundant in controls, whereas Pucciniomycetes increased in soil with litter from transformed trees (P = 0.051). Richness estimators and diversity indices revealed no significant difference in the composition of fungal communities; PCoA partitioned the fungal communities into three groups: (i) those with higher amounts of added tannin from both transformed and untransformed treatments, (ii) those corresponding to soils without litter, and (iii) those corresponding to microcosms with litter added from trees transformed only with a β-glucuronidase (GUS) control vector. While the litter from transformed poplars had significant effects on soil microbe communities, the observed impacts reflected known impacts on soil processes associated with tannins, and were similar to changes that would be expected from natural variation in tannin levels. |
| first_indexed | 2024-12-22T08:53:47Z |
| format | Article |
| id | doaj.art-746d0d23ec1147b6882981d0a924af04 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T08:53:47Z |
| publishDate | 2013-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-746d0d23ec1147b6882981d0a924af042022-12-21T18:31:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2013-09-01410.3389/fmicb.2013.0029054207The effects of high-tannin leaf litter from transgenic poplars on microbial communities in microcosm soils.Richard S. Winder0Josyanne eLamarche1C. Peter eConstabel2Richard eHamelin3Natural Resources CanadaRessources naturelles CanadaUniversity of VictoriaRessources naturelles CanadaThe impacts of leaf litter from genetically-modified hybrid poplar accumulating high levels of condensed tannins (proanthocyanidins) were examined in soil microcosms consisting of moss growing on sieved soil. Moss preferentially proliferated in microcosms with lower tannin content; DGGE detected increased fungal diversity in microcosms with low-tannin litter. The proportion of cloned rDNA sequences from Actinobacteria decreased with litter addition while Bacteroidetes, Chloroflexi, Cyanobacteria, and α-Proteobacteria significantly increased. β–Proteobacteria were proportionally more numerous at high tannin levels. Tannins had no significant impact on overall diversity of bacterial communities analyzed with various estimators. There was an increased proportion of N-fixing bacteria corresponding to the addition of litter with low tannin levels. The addition of litter increased the proportion of Ascomycota/Basidiomycota. Dothideomycetes, Pucciniomycetes, and Tremellomycetes also increased and Agaricomycetes decreased. Agaricomycetes and Sordariomycetes were significantly more abundant in controls, whereas Pucciniomycetes increased in soil with litter from transformed trees (P = 0.051). Richness estimators and diversity indices revealed no significant difference in the composition of fungal communities; PCoA partitioned the fungal communities into three groups: (i) those with higher amounts of added tannin from both transformed and untransformed treatments, (ii) those corresponding to soils without litter, and (iii) those corresponding to microcosms with litter added from trees transformed only with a β-glucuronidase (GUS) control vector. While the litter from transformed poplars had significant effects on soil microbe communities, the observed impacts reflected known impacts on soil processes associated with tannins, and were similar to changes that would be expected from natural variation in tannin levels.http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00290/fullForestryNitrogen CycleRisk AssessmenttransgenicTreePopulus tremuloides |
| spellingShingle | Richard S. Winder Josyanne eLamarche C. Peter eConstabel Richard eHamelin The effects of high-tannin leaf litter from transgenic poplars on microbial communities in microcosm soils. Frontiers in Microbiology Forestry Nitrogen Cycle Risk Assessment transgenic Tree Populus tremuloides |
| title | The effects of high-tannin leaf litter from transgenic poplars on microbial communities in microcosm soils. |
| title_full | The effects of high-tannin leaf litter from transgenic poplars on microbial communities in microcosm soils. |
| title_fullStr | The effects of high-tannin leaf litter from transgenic poplars on microbial communities in microcosm soils. |
| title_full_unstemmed | The effects of high-tannin leaf litter from transgenic poplars on microbial communities in microcosm soils. |
| title_short | The effects of high-tannin leaf litter from transgenic poplars on microbial communities in microcosm soils. |
| title_sort | effects of high tannin leaf litter from transgenic poplars on microbial communities in microcosm soils |
| topic | Forestry Nitrogen Cycle Risk Assessment transgenic Tree Populus tremuloides |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00290/full |
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