Biodiversity of phytoplankton cyst assemblages in surface sediments of the Black Sea based on metabarcoding
Resting stages are common for the life cycle of some phytoplankton species, including blooming and potentially toxic species. The “seed bank” accumulated in the sediments can initiate blooms in the water column and could be an early warning signal of harmful algal blooms (HABs). In order to identify...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-11-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2018.1532816 |
| _version_ | 1818527131093696512 |
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| author | Nina Dzhembekova Snejana Moncheva Petya Ivanova Nataliya Slabakova Satoshi Nagai |
| author_facet | Nina Dzhembekova Snejana Moncheva Petya Ivanova Nataliya Slabakova Satoshi Nagai |
| author_sort | Nina Dzhembekova |
| collection | DOAJ |
| description | Resting stages are common for the life cycle of some phytoplankton species, including blooming and potentially toxic species. The “seed bank” accumulated in the sediments can initiate blooms in the water column and could be an early warning signal of harmful algal blooms (HABs). In order to identify the phytoplankton cyst assemblages, thirteen surface sediment samples were collected from different sites in the Black Sea. The diversity of the resting stages was assessed using high-throughput sequencing metabarcoding (V7-9 hypervariable region of the 18S rDNA). One hundred and eighty microalgal species were identified with high level of similarity to the reference sequences. Dinoflagellates were dominated by Biecheleria, Gymnodinium and Karlodinium. Within diatoms, Skeletonema, Chaetoceros and Thalassiosira were the most abundant genera. Sixteen of the detected operational taxonomic units (OTUs) were assigned to harmful microalgae (12 dinoflagellates species, 1 diatom, 1 haptophyte and 2 raphidophytes). No pattern of microalgal sequences depth distribution was discriminated. The results show that DNA metabarcoding has a great potential for assessment of the phytoplankton diversity in environmental sediments. |
| first_indexed | 2024-12-11T06:32:07Z |
| format | Article |
| id | doaj.art-746d5cbecd4d4240abeeed0fef781db6 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-11T06:32:07Z |
| publishDate | 2018-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-746d5cbecd4d4240abeeed0fef781db62022-12-22T01:17:29ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302018-11-013261507151310.1080/13102818.2018.15328161532816Biodiversity of phytoplankton cyst assemblages in surface sediments of the Black Sea based on metabarcodingNina Dzhembekova0Snejana Moncheva1Petya Ivanova2Nataliya Slabakova3Satoshi Nagai4Institute of Oceanology, Bulgarian Academy of SciencesInstitute of Oceanology, Bulgarian Academy of SciencesInstitute of Oceanology, Bulgarian Academy of SciencesInstitute of Oceanology, Bulgarian Academy of SciencesResearch Center for Aquatic Genomics, National Research Institute of Fisheries Science, Japan Fisheries Research and Education AgencyResting stages are common for the life cycle of some phytoplankton species, including blooming and potentially toxic species. The “seed bank” accumulated in the sediments can initiate blooms in the water column and could be an early warning signal of harmful algal blooms (HABs). In order to identify the phytoplankton cyst assemblages, thirteen surface sediment samples were collected from different sites in the Black Sea. The diversity of the resting stages was assessed using high-throughput sequencing metabarcoding (V7-9 hypervariable region of the 18S rDNA). One hundred and eighty microalgal species were identified with high level of similarity to the reference sequences. Dinoflagellates were dominated by Biecheleria, Gymnodinium and Karlodinium. Within diatoms, Skeletonema, Chaetoceros and Thalassiosira were the most abundant genera. Sixteen of the detected operational taxonomic units (OTUs) were assigned to harmful microalgae (12 dinoflagellates species, 1 diatom, 1 haptophyte and 2 raphidophytes). No pattern of microalgal sequences depth distribution was discriminated. The results show that DNA metabarcoding has a great potential for assessment of the phytoplankton diversity in environmental sediments.http://dx.doi.org/10.1080/13102818.2018.153281618S rDNAresting stagessediment eukaryotic biodiversityhigh-throughput sequencing (HTS) |
| spellingShingle | Nina Dzhembekova Snejana Moncheva Petya Ivanova Nataliya Slabakova Satoshi Nagai Biodiversity of phytoplankton cyst assemblages in surface sediments of the Black Sea based on metabarcoding Biotechnology & Biotechnological Equipment 18S rDNA resting stages sediment eukaryotic biodiversity high-throughput sequencing (HTS) |
| title | Biodiversity of phytoplankton cyst assemblages in surface sediments of the Black Sea based on metabarcoding |
| title_full | Biodiversity of phytoplankton cyst assemblages in surface sediments of the Black Sea based on metabarcoding |
| title_fullStr | Biodiversity of phytoplankton cyst assemblages in surface sediments of the Black Sea based on metabarcoding |
| title_full_unstemmed | Biodiversity of phytoplankton cyst assemblages in surface sediments of the Black Sea based on metabarcoding |
| title_short | Biodiversity of phytoplankton cyst assemblages in surface sediments of the Black Sea based on metabarcoding |
| title_sort | biodiversity of phytoplankton cyst assemblages in surface sediments of the black sea based on metabarcoding |
| topic | 18S rDNA resting stages sediment eukaryotic biodiversity high-throughput sequencing (HTS) |
| url | http://dx.doi.org/10.1080/13102818.2018.1532816 |
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