HPTLC-DPPH显色-像素分析快速测定酚类 抗氧化剂:以食用油和烘焙食品中TBHQ为例HPTLC-DPPH colorimetric-bioautographic image quantification of phenolic antioxidant: taking TBHQ in edible oil and baking foods as an example
目前,酚类抗氧化剂TBHQ的常用检测方法存在前处理烦琐、耗时长、单次只能测定一个样品的问题,因此亟需建立一种高通量、快速准确的检测方法。为此,将高效薄层色谱(HPTLC)分离与1,1-二苯基-2-三硝基苯肼(DPPH)还原变色反应、图像像素定量分析相结合,建立了一种快速筛检酚类抗氧化剂TBHQ的方法。在此基础上,以食用油、面包和蛋糕为代表性样品,验证所建方法的实际应用性能。结果显示:所建方法检测TBHQ的检测限为70 ng/zone (35 mg/kg),定量限为140 ng/zone (70 mg/kg),定量限低于GB 2760—2014规定的使用限量(200 mg/kg);通过对比分析,...
Main Author: | 卫晓1,席兴军2,李慧3,王小三1,黄丹菲4,徐学明1,陈启飞1,黄晓倩1,陈益胜1 WEI Xiao1, XI Xingjun2, LI Hui3, WANG Xiaosan1, HUANG Danfei4, XU Xueming1, CHEN Qifei1, HUANG Xiaoqian1, CHEN Yisheng1 |
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Format: | Article |
Language: | English |
Published: |
中粮工科(西安)国际工程有限公司
2022-03-01
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Series: | Zhongguo youzhi |
Subjects: | |
Online Access: | http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220318&year_id=2022&quarter_id=3&falg=1 |
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