Anthocyanins from <i>Opuntia ficus-indica</i> Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production
<i>Opuntia ficus-indica</i> is rich in a variety of active substances, such as anthocyanins, flavonoids, and polysaccharides. Some studies have shown that anthocyanins extracted from natural plants can regulate intestinal flora. The fruit was used as raw material, and anthocyanins were e...
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MDPI AG
2022-10-01
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| author | Yun Zhang Huan Chang Shuai Shao Lin Zhao Ruiying Zhang Shouwen Zhang |
| author_facet | Yun Zhang Huan Chang Shuai Shao Lin Zhao Ruiying Zhang Shouwen Zhang |
| author_sort | Yun Zhang |
| collection | DOAJ |
| description | <i>Opuntia ficus-indica</i> is rich in a variety of active substances, such as anthocyanins, flavonoids, and polysaccharides. Some studies have shown that anthocyanins extracted from natural plants can regulate intestinal flora. The fruit was used as raw material, and anthocyanins were extracted from it. In vivo experiments were used to study the effect of <i>Opuntia ficus-indica</i> anthocyanins on the mouse intestine by 16S rRNA high-throughput sequencing (NovaSeq 6000 platform) and gas chromatography (hydrogen flame ionization detector (FID)) methods. Microbiota and effects of short-chain fatty acids (SCFAs). The results showed that after feeding anthocyanins, the diversity of intestinal microorganisms in mice was significantly increased (<i>p</i> < 0.05), the ratio of Firmicutes/Bacteroidetes (F/B value) was significantly decreased (<i>p</i> < 0.05), the relative abundances of beneficial bacteria Lactobacillus, Bifidobacterium, Prevotella, and Akkermansia in the intestinal tract of mice were significantly increased (<i>p</i> < 0.05), and the relative abundance of pathogenic bacteria Escherichia-Shigella and Desulfovibrio decreased significantly (<i>p</i> < 0.05). Furthermore, anthocyanins significantly increased the content of short-chain fatty acids in the cecum of mice, among which the content of acetic acid, propionic acid, and butyric acid increased the most. <i>Opuntia ficus-indica</i> anthocyanins can change the microbial diversity and flora composition of the mouse gut and promote the production of short-chain fatty acids. The findings provide a theoretical basis for the use of <i>Opuntia ficus-indica</i> anthocyanins as dietary supplements to regulate human intestinal flora. |
| first_indexed | 2024-03-09T20:39:54Z |
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| language | English |
| last_indexed | 2024-03-09T20:39:54Z |
| publishDate | 2022-10-01 |
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| series | Biology |
| spelling | doaj.art-746e057e901a45048132c7adb20f4db12023-11-23T23:00:41ZengMDPI AGBiology2079-77372022-10-011110150510.3390/biology11101505Anthocyanins from <i>Opuntia ficus-indica</i> Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid ProductionYun Zhang0Huan Chang1Shuai Shao2Lin Zhao3Ruiying Zhang4Shouwen Zhang5Postdoctoral Research Station, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaCollege of Food Engineering, Heilongjiang East University, Harbin 150066, ChinaCollege of Food Engineering, Heilongjiang East University, Harbin 150066, ChinaPostdoctoral Research Station, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaPostdoctoral Research Station, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaCollege of Food Engineering, Heilongjiang East University, Harbin 150066, China<i>Opuntia ficus-indica</i> is rich in a variety of active substances, such as anthocyanins, flavonoids, and polysaccharides. Some studies have shown that anthocyanins extracted from natural plants can regulate intestinal flora. The fruit was used as raw material, and anthocyanins were extracted from it. In vivo experiments were used to study the effect of <i>Opuntia ficus-indica</i> anthocyanins on the mouse intestine by 16S rRNA high-throughput sequencing (NovaSeq 6000 platform) and gas chromatography (hydrogen flame ionization detector (FID)) methods. Microbiota and effects of short-chain fatty acids (SCFAs). The results showed that after feeding anthocyanins, the diversity of intestinal microorganisms in mice was significantly increased (<i>p</i> < 0.05), the ratio of Firmicutes/Bacteroidetes (F/B value) was significantly decreased (<i>p</i> < 0.05), the relative abundances of beneficial bacteria Lactobacillus, Bifidobacterium, Prevotella, and Akkermansia in the intestinal tract of mice were significantly increased (<i>p</i> < 0.05), and the relative abundance of pathogenic bacteria Escherichia-Shigella and Desulfovibrio decreased significantly (<i>p</i> < 0.05). Furthermore, anthocyanins significantly increased the content of short-chain fatty acids in the cecum of mice, among which the content of acetic acid, propionic acid, and butyric acid increased the most. <i>Opuntia ficus-indica</i> anthocyanins can change the microbial diversity and flora composition of the mouse gut and promote the production of short-chain fatty acids. The findings provide a theoretical basis for the use of <i>Opuntia ficus-indica</i> anthocyanins as dietary supplements to regulate human intestinal flora.https://www.mdpi.com/2079-7737/11/10/1505<i>Opuntia ficus-indica</i>anthocyaninsgut microbiotahigh-throughput sequencingSCFAs |
| spellingShingle | Yun Zhang Huan Chang Shuai Shao Lin Zhao Ruiying Zhang Shouwen Zhang Anthocyanins from <i>Opuntia ficus-indica</i> Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production Biology <i>Opuntia ficus-indica</i> anthocyanins gut microbiota high-throughput sequencing SCFAs |
| title | Anthocyanins from <i>Opuntia ficus-indica</i> Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production |
| title_full | Anthocyanins from <i>Opuntia ficus-indica</i> Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production |
| title_fullStr | Anthocyanins from <i>Opuntia ficus-indica</i> Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production |
| title_full_unstemmed | Anthocyanins from <i>Opuntia ficus-indica</i> Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production |
| title_short | Anthocyanins from <i>Opuntia ficus-indica</i> Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production |
| title_sort | anthocyanins from i opuntia ficus indica i modulate gut microbiota composition and improve short chain fatty acid production |
| topic | <i>Opuntia ficus-indica</i> anthocyanins gut microbiota high-throughput sequencing SCFAs |
| url | https://www.mdpi.com/2079-7737/11/10/1505 |
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