FAM60A promotes osteosarcoma development and progression
Abstract Background Osteosarcoma (OS) is a highly malignant primary bone tumor. Family of homology 60A (FAM60A) reportedly contributes to the malignant growth of some tumors. Methods Herein we investigated the mRNA expression level of FAM60A by combining OS and non‐cancer samples from public databas...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-08-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.6343 |
| _version_ | 1797242689394049024 |
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| author | Yu Sun Yu‐Nan Man Jin‐hui Cheng Jing‐tang Li Ya‐yun Liu |
| author_facet | Yu Sun Yu‐Nan Man Jin‐hui Cheng Jing‐tang Li Ya‐yun Liu |
| author_sort | Yu Sun |
| collection | DOAJ |
| description | Abstract Background Osteosarcoma (OS) is a highly malignant primary bone tumor. Family of homology 60A (FAM60A) reportedly contributes to the malignant growth of some tumors. Methods Herein we investigated the mRNA expression level of FAM60A by combining OS and non‐cancer samples from public databases. Immunohistochemistry was performed to determine protein expression levels of FAM60A in patients with OS. Further, RT‐qPCR and western blotting were conducted to evaluate FAM60A expression in various OS cell lines. CCK‐8 assay, colony formation assay, and flow cytometry were applied to determine the function of FAM60A. Finally, functional enrichment analysis was performed based on FAM60A co‐expressed genes. Results FAM60A mRNA expression level was found to be significantly upregulated (standardized mean difference = 1.27, 95% CI [0.67–1.88]). Survival analyses suggested that higher expression of FAM60A was indicative of poor prognoses. Similarly, FAM60A protein expression level was also observed to be upregulated. Knocking down FAM60A expression inhibited OS cell proliferation, increased apoptosis, and blocked cells from entering the S phase. Besides, cell cycle was the most prominently enriched pathway, and BUB1, DTL, and EXO1 were identified as hub genes. Conclusions FAM60A expression was found to be markedly upregulated in OS; furthermore, FAM60A was observed to promote OS cell proliferation, inhibit apoptosis, and participate in cell cycle regulation. Besides, FAM60A may interact with hub genes to participate in the progress of OS. |
| first_indexed | 2024-03-12T00:55:12Z |
| format | Article |
| id | doaj.art-746f1253606f444c859c9be0c5d93452 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-04-24T18:43:13Z |
| publishDate | 2023-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-746f1253606f444c859c9be0c5d934522024-03-27T09:11:01ZengWileyCancer Medicine2045-76342023-08-011216174911750310.1002/cam4.6343FAM60A promotes osteosarcoma development and progressionYu Sun0Yu‐Nan Man1Jin‐hui Cheng2Jing‐tang Li3Ya‐yun Liu4Division of Spinal Surgery The First Affiliated Hospital of Guangxi Medical University Nanning Guangxi Zhuang Autonomous Region P.R. ChinaDivision of Spinal Surgery The First Affiliated Hospital of Guangxi Medical University Nanning Guangxi Zhuang Autonomous Region P.R. ChinaJiangxi Provincial People's Hospital The First Affiliated Hospital of Nanchang Medical College Nanchang Jiangxi ChinaJiangxi Provincial People's Hospital The First Affiliated Hospital of Nanchang Medical College Nanchang Jiangxi ChinaJiangxi Provincial People's Hospital The First Affiliated Hospital of Nanchang Medical College Nanchang Jiangxi ChinaAbstract Background Osteosarcoma (OS) is a highly malignant primary bone tumor. Family of homology 60A (FAM60A) reportedly contributes to the malignant growth of some tumors. Methods Herein we investigated the mRNA expression level of FAM60A by combining OS and non‐cancer samples from public databases. Immunohistochemistry was performed to determine protein expression levels of FAM60A in patients with OS. Further, RT‐qPCR and western blotting were conducted to evaluate FAM60A expression in various OS cell lines. CCK‐8 assay, colony formation assay, and flow cytometry were applied to determine the function of FAM60A. Finally, functional enrichment analysis was performed based on FAM60A co‐expressed genes. Results FAM60A mRNA expression level was found to be significantly upregulated (standardized mean difference = 1.27, 95% CI [0.67–1.88]). Survival analyses suggested that higher expression of FAM60A was indicative of poor prognoses. Similarly, FAM60A protein expression level was also observed to be upregulated. Knocking down FAM60A expression inhibited OS cell proliferation, increased apoptosis, and blocked cells from entering the S phase. Besides, cell cycle was the most prominently enriched pathway, and BUB1, DTL, and EXO1 were identified as hub genes. Conclusions FAM60A expression was found to be markedly upregulated in OS; furthermore, FAM60A was observed to promote OS cell proliferation, inhibit apoptosis, and participate in cell cycle regulation. Besides, FAM60A may interact with hub genes to participate in the progress of OS.https://doi.org/10.1002/cam4.6343clinical significancefamily of homology 60Ahub genesosteosarcoma |
| spellingShingle | Yu Sun Yu‐Nan Man Jin‐hui Cheng Jing‐tang Li Ya‐yun Liu FAM60A promotes osteosarcoma development and progression Cancer Medicine clinical significance family of homology 60A hub genes osteosarcoma |
| title | FAM60A promotes osteosarcoma development and progression |
| title_full | FAM60A promotes osteosarcoma development and progression |
| title_fullStr | FAM60A promotes osteosarcoma development and progression |
| title_full_unstemmed | FAM60A promotes osteosarcoma development and progression |
| title_short | FAM60A promotes osteosarcoma development and progression |
| title_sort | fam60a promotes osteosarcoma development and progression |
| topic | clinical significance family of homology 60A hub genes osteosarcoma |
| url | https://doi.org/10.1002/cam4.6343 |
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