Isolation and Characterization of a Novel <i>Autographiviridae</i> Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant <i>Acinetobacter baumannii</i>-Associated Skin and Soft Tissue Infections
Multidrug-resistant <i>Acinetobacter baumannii</i> (MDR <i>A. baumannii</i>) is one of the ESKAPE pathogens that restricts available treatment options. MDR <i>A. baumannii</i> is responsible for a dramatic increase in case numbers of a wide variety of infections,...
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MDPI AG
2022-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/2/194 |
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| author | Phitchayapak Wintachai Komwit Surachat Kamonnut Singkhamanan |
| author_facet | Phitchayapak Wintachai Komwit Surachat Kamonnut Singkhamanan |
| author_sort | Phitchayapak Wintachai |
| collection | DOAJ |
| description | Multidrug-resistant <i>Acinetobacter baumannii</i> (MDR <i>A. baumannii</i>) is one of the ESKAPE pathogens that restricts available treatment options. MDR <i>A. baumannii</i> is responsible for a dramatic increase in case numbers of a wide variety of infections, including skin and soft tissue infections (SSTIs), resulting in pyoderma, surgical debridement, and necrotizing fasciitis. To investigate an alternative medical treatment for SSTIs, a broad range lytic <i>Acinetobacter</i> phage, vB _AbP_ABWU2101 (phage vABWU2101), for lysing MDR <i>A. baumannii</i> in associated SSTIs was isolated and the biological aspects of this phage were investigated. Morphological characterization and genomic analysis revealed that phage vABWU2101 was a new species in the <i>Friunavirus</i>, <i>Beijerinckvirinae</i>, family <i>Autographiviridae</i>, and order <i>Caudovirales</i>. Antibiofilm activity of phage vABWU2101 demonstrated good activity against both preformed biofilms and biofilm formation. The combination of phage vABWU2101 and tigecycline showed synergistic antimicrobial activities against planktonic and biofilm cells. Scanning electron microscopy confirmed that the antibacterial efficacy of the combination of phage vABWU2101 and tigecycline was more effective than the phage or antibiotic alone. Hence, our findings could potentially be used to develop a therapeutic option for the treatment of SSTIs caused by MDR <i>A. baumannii.</i> |
| first_indexed | 2024-03-09T20:52:37Z |
| format | Article |
| id | doaj.art-7470464a74da42e6bf34bdd31d9cafd5 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T20:52:37Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-7470464a74da42e6bf34bdd31d9cafd52023-11-23T22:29:02ZengMDPI AGViruses1999-49152022-01-0114219410.3390/v14020194Isolation and Characterization of a Novel <i>Autographiviridae</i> Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant <i>Acinetobacter baumannii</i>-Associated Skin and Soft Tissue InfectionsPhitchayapak Wintachai0Komwit Surachat1Kamonnut Singkhamanan2School of Science, Walailak University, Nakhon Si Thammarat 80161, ThailandDivision of Computational Science, Faculty of Science, Prince of Songkla University, Hat Yai 90110, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, ThailandMultidrug-resistant <i>Acinetobacter baumannii</i> (MDR <i>A. baumannii</i>) is one of the ESKAPE pathogens that restricts available treatment options. MDR <i>A. baumannii</i> is responsible for a dramatic increase in case numbers of a wide variety of infections, including skin and soft tissue infections (SSTIs), resulting in pyoderma, surgical debridement, and necrotizing fasciitis. To investigate an alternative medical treatment for SSTIs, a broad range lytic <i>Acinetobacter</i> phage, vB _AbP_ABWU2101 (phage vABWU2101), for lysing MDR <i>A. baumannii</i> in associated SSTIs was isolated and the biological aspects of this phage were investigated. Morphological characterization and genomic analysis revealed that phage vABWU2101 was a new species in the <i>Friunavirus</i>, <i>Beijerinckvirinae</i>, family <i>Autographiviridae</i>, and order <i>Caudovirales</i>. Antibiofilm activity of phage vABWU2101 demonstrated good activity against both preformed biofilms and biofilm formation. The combination of phage vABWU2101 and tigecycline showed synergistic antimicrobial activities against planktonic and biofilm cells. Scanning electron microscopy confirmed that the antibacterial efficacy of the combination of phage vABWU2101 and tigecycline was more effective than the phage or antibiotic alone. Hence, our findings could potentially be used to develop a therapeutic option for the treatment of SSTIs caused by MDR <i>A. baumannii.</i>https://www.mdpi.com/1999-4915/14/2/194<i>Autographiviridae</i> phage<i>Acinetobacter baumannii</i>bacteriophageantibacterial activitybiofilmssynergistic activity |
| spellingShingle | Phitchayapak Wintachai Komwit Surachat Kamonnut Singkhamanan Isolation and Characterization of a Novel <i>Autographiviridae</i> Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant <i>Acinetobacter baumannii</i>-Associated Skin and Soft Tissue Infections Viruses <i>Autographiviridae</i> phage <i>Acinetobacter baumannii</i> bacteriophage antibacterial activity biofilms synergistic activity |
| title | Isolation and Characterization of a Novel <i>Autographiviridae</i> Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant <i>Acinetobacter baumannii</i>-Associated Skin and Soft Tissue Infections |
| title_full | Isolation and Characterization of a Novel <i>Autographiviridae</i> Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant <i>Acinetobacter baumannii</i>-Associated Skin and Soft Tissue Infections |
| title_fullStr | Isolation and Characterization of a Novel <i>Autographiviridae</i> Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant <i>Acinetobacter baumannii</i>-Associated Skin and Soft Tissue Infections |
| title_full_unstemmed | Isolation and Characterization of a Novel <i>Autographiviridae</i> Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant <i>Acinetobacter baumannii</i>-Associated Skin and Soft Tissue Infections |
| title_short | Isolation and Characterization of a Novel <i>Autographiviridae</i> Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant <i>Acinetobacter baumannii</i>-Associated Skin and Soft Tissue Infections |
| title_sort | isolation and characterization of a novel i autographiviridae i phage and its combined effect with tigecycline in controlling multidrug resistant i acinetobacter baumannii i associated skin and soft tissue infections |
| topic | <i>Autographiviridae</i> phage <i>Acinetobacter baumannii</i> bacteriophage antibacterial activity biofilms synergistic activity |
| url | https://www.mdpi.com/1999-4915/14/2/194 |
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