Effects of estradiol on the virulence traits of Porphyromonas gingivalis
Abstract Porphyromonas gingivalis has been strongly associated to active periodontitis sites. A number of studies have tried to elucidate the association between female steroid sex hormones and gingival health. However, until now, there is limited knowledge on estradiol effects on the virulence trai...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-17019-z |
| _version_ | 1818517252203347968 |
|---|---|
| author | Kartheyaene Jayaprakash Demirel Alessandra Neves Guimaraes Isak Demirel |
| author_facet | Kartheyaene Jayaprakash Demirel Alessandra Neves Guimaraes Isak Demirel |
| author_sort | Kartheyaene Jayaprakash Demirel |
| collection | DOAJ |
| description | Abstract Porphyromonas gingivalis has been strongly associated to active periodontitis sites. A number of studies have tried to elucidate the association between female steroid sex hormones and gingival health. However, until now, there is limited knowledge on estradiol effects on the virulence traits of P. gingivalis. The aim of the study was to investigate the impact of estradiol exposure on the virulence characteristics of P. gingivalis strain W50. We found that a pre- and postmenopausal concentration of estradiol increased the growth and biofilm formation of P. gingivalis W50. We also found that estradiol increased the release of lysine and arginine gingipains from W50. We then showed that IL-1β, CXCL10 and TGF-β1 release from gingival epithelial cells was significantly lowered by W50 pre-exposed to estradiol compared to W50 alone. Real time-qPCR showed that the gene expression of IL-18, IL-6, IL-8, TGF-β1 and NLRP3 in gingival epithelial cells was significantly lowered by W50 pre-exposed to estradiol compared to W50 alone. We also found that estradiol in a dose-dependent manner increased P. gingivalis colonization and invasion of gingival epithelial cells. Taken together, our findings show that estradiol has the ability to alter the virulence traits of P. gingivalis. |
| first_indexed | 2024-12-11T00:53:46Z |
| format | Article |
| id | doaj.art-74706c95358c4c36bfeb6681d8e7c41e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-11T00:53:46Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-74706c95358c4c36bfeb6681d8e7c41e2022-12-22T01:26:33ZengNature PortfolioScientific Reports2045-23222022-08-0112111010.1038/s41598-022-17019-zEffects of estradiol on the virulence traits of Porphyromonas gingivalisKartheyaene Jayaprakash Demirel0Alessandra Neves Guimaraes1Isak Demirel2Department of Odontological Research, Public Dental Service, Faculty of Medicine and Health, Örebro University, Campus USÖDepartment of Periodontology and Implantology, Public Dental Service, Faculty of Medicine and Health, Örebro UniversityiRiSC-Inflammatory Response and Infection Susceptibility Centre, Faculty of Medicine and Health, Örebro UniversityAbstract Porphyromonas gingivalis has been strongly associated to active periodontitis sites. A number of studies have tried to elucidate the association between female steroid sex hormones and gingival health. However, until now, there is limited knowledge on estradiol effects on the virulence traits of P. gingivalis. The aim of the study was to investigate the impact of estradiol exposure on the virulence characteristics of P. gingivalis strain W50. We found that a pre- and postmenopausal concentration of estradiol increased the growth and biofilm formation of P. gingivalis W50. We also found that estradiol increased the release of lysine and arginine gingipains from W50. We then showed that IL-1β, CXCL10 and TGF-β1 release from gingival epithelial cells was significantly lowered by W50 pre-exposed to estradiol compared to W50 alone. Real time-qPCR showed that the gene expression of IL-18, IL-6, IL-8, TGF-β1 and NLRP3 in gingival epithelial cells was significantly lowered by W50 pre-exposed to estradiol compared to W50 alone. We also found that estradiol in a dose-dependent manner increased P. gingivalis colonization and invasion of gingival epithelial cells. Taken together, our findings show that estradiol has the ability to alter the virulence traits of P. gingivalis.https://doi.org/10.1038/s41598-022-17019-z |
| spellingShingle | Kartheyaene Jayaprakash Demirel Alessandra Neves Guimaraes Isak Demirel Effects of estradiol on the virulence traits of Porphyromonas gingivalis Scientific Reports |
| title | Effects of estradiol on the virulence traits of Porphyromonas gingivalis |
| title_full | Effects of estradiol on the virulence traits of Porphyromonas gingivalis |
| title_fullStr | Effects of estradiol on the virulence traits of Porphyromonas gingivalis |
| title_full_unstemmed | Effects of estradiol on the virulence traits of Porphyromonas gingivalis |
| title_short | Effects of estradiol on the virulence traits of Porphyromonas gingivalis |
| title_sort | effects of estradiol on the virulence traits of porphyromonas gingivalis |
| url | https://doi.org/10.1038/s41598-022-17019-z |
| work_keys_str_mv | AT kartheyaenejayaprakashdemirel effectsofestradiolonthevirulencetraitsofporphyromonasgingivalis AT alessandranevesguimaraes effectsofestradiolonthevirulencetraitsofporphyromonasgingivalis AT isakdemirel effectsofestradiolonthevirulencetraitsofporphyromonasgingivalis |