Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease
Abstract Background The gp85 is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization. Here, we mapped the epitope in ALV-J gp85 by ELISA using synthetic peptides and developed epitope based diagnostic methods for ALV-J infection. Results The results reveale...
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1622-x |
| _version_ | 1818289812906442752 |
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| author | Kun Qian Xue Tian Hongxia Shao Jianqiang Ye Yongxiu Yao Venugopal Nair Aijian Qin |
| author_facet | Kun Qian Xue Tian Hongxia Shao Jianqiang Ye Yongxiu Yao Venugopal Nair Aijian Qin |
| author_sort | Kun Qian |
| collection | DOAJ |
| description | Abstract Background The gp85 is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization. Here, we mapped the epitope in ALV-J gp85 by ELISA using synthetic peptides and developed epitope based diagnostic methods for ALV-J infection. Results The results revealed that monoclonal antibody (mAb) JE9 recognized 83WDPQEL88 motif, which was highly conserved in gp85 among different ALV-J strains by homology analysis. Moreover, after evaluation with two hundred and forty sera samples obtained from different chicken farms, the epitope-based peptide ELISA had much higher sensitivity than commercial ELISA kit for antibody detection of ALV-J. Conclusions A novel B-cell epitope recognized by the mAb JE9 was identified. The developed peptide-ELISA based on this novel B-cell epitope could be useful in laboratory viral diagnosis, routine surveillance in chicken farms, and also in understanding the pathogenesis of ALV-J. |
| first_indexed | 2024-12-13T02:18:14Z |
| format | Article |
| id | doaj.art-7470ae920546492d92c3d8c62646b1df |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-13T02:18:14Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-7470ae920546492d92c3d8c62646b1df2022-12-22T00:02:50ZengBMCBMC Veterinary Research1746-61482018-09-011411710.1186/s12917-018-1622-xIdentification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of diseaseKun Qian0Xue Tian1Hongxia Shao2Jianqiang Ye3Yongxiu Yao4Venugopal Nair5Aijian Qin6Ministry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou UniversityMinistry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou UniversityMinistry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou UniversityMinistry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou UniversityAvian Oncogenic Virus Group, The Pirbright InstituteAvian Oncogenic Virus Group, The Pirbright InstituteMinistry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou UniversityAbstract Background The gp85 is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization. Here, we mapped the epitope in ALV-J gp85 by ELISA using synthetic peptides and developed epitope based diagnostic methods for ALV-J infection. Results The results revealed that monoclonal antibody (mAb) JE9 recognized 83WDPQEL88 motif, which was highly conserved in gp85 among different ALV-J strains by homology analysis. Moreover, after evaluation with two hundred and forty sera samples obtained from different chicken farms, the epitope-based peptide ELISA had much higher sensitivity than commercial ELISA kit for antibody detection of ALV-J. Conclusions A novel B-cell epitope recognized by the mAb JE9 was identified. The developed peptide-ELISA based on this novel B-cell epitope could be useful in laboratory viral diagnosis, routine surveillance in chicken farms, and also in understanding the pathogenesis of ALV-J.http://link.springer.com/article/10.1186/s12917-018-1622-xAvian leukosis virus subgroup JB-cell epitope mappingEpitope-based peptide ELISAAntibody detection |
| spellingShingle | Kun Qian Xue Tian Hongxia Shao Jianqiang Ye Yongxiu Yao Venugopal Nair Aijian Qin Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease BMC Veterinary Research Avian leukosis virus subgroup J B-cell epitope mapping Epitope-based peptide ELISA Antibody detection |
| title | Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease |
| title_full | Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease |
| title_fullStr | Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease |
| title_full_unstemmed | Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease |
| title_short | Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease |
| title_sort | identification of novel b cell epitope in gp85 of subgroup j avian leukosis virus and its application in diagnosis of disease |
| topic | Avian leukosis virus subgroup J B-cell epitope mapping Epitope-based peptide ELISA Antibody detection |
| url | http://link.springer.com/article/10.1186/s12917-018-1622-x |
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