Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis
Rapid profiling of the biomolecular components of milk can be useful for food quality assessment and for food fraud detection. Differences in commercial value and availability of milk from specific species are often the reasons for the illicit and fraudulent sale of milk whose species origin is wron...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Proteomes |
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| Online Access: | https://www.mdpi.com/2227-7382/8/3/20 |
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| author | Cristian Piras Carlotta Ceniti Evita Hartmane Nicola Costanzo Valeria Maria Morittu Paola Roncada Domenico Britti Rainer Cramer |
| author_facet | Cristian Piras Carlotta Ceniti Evita Hartmane Nicola Costanzo Valeria Maria Morittu Paola Roncada Domenico Britti Rainer Cramer |
| author_sort | Cristian Piras |
| collection | DOAJ |
| description | Rapid profiling of the biomolecular components of milk can be useful for food quality assessment and for food fraud detection. Differences in commercial value and availability of milk from specific species are often the reasons for the illicit and fraudulent sale of milk whose species origin is wrongly declared. In this study, a fast, MS-based speciation method is presented to distinguish sheep from goat milk and sheep colostrum at different phases. Using liquid atmospheric pressure (AP)-matrix-assisted laser desorption/ionisation (MALDI) MS, it was possible to classify samples of goat and sheep milk with 100% accuracy in one minute of data acquisition per sample. Moreover, an accuracy of 98% was achieved in classifying pure sheep milk samples and sheep milk samples containing 10% goat milk. Evaluating colostrum quality and postnatal stages represents another possible application of this technology. Classification of sheep colostrum samples that were collected within 6 hours after parturition and 48 hours later was achieved with an accuracy of 84.4%. Our data show that substantial changes in the lipid profile can account for the accurate classification of colostrum collected at the early and late time points. This method applied to the analysis of protein orthologs of different species can, as in this case, allow unequivocal speciation analysis. |
| first_indexed | 2024-03-10T17:03:56Z |
| format | Article |
| id | doaj.art-74765bec69cf4424ba1dcfb165f9c7e5 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-03-10T17:03:56Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-74765bec69cf4424ba1dcfb165f9c7e52023-11-20T10:52:33ZengMDPI AGProteomes2227-73822020-08-01832010.3390/proteomes8030020Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum AnalysisCristian Piras0Carlotta Ceniti1Evita Hartmane2Nicola Costanzo3Valeria Maria Morittu4Paola Roncada5Domenico Britti6Rainer Cramer7Department of Chemistry, University of Reading, Reading RG6 6DX, UKDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, ItalyDepartment of Chemistry, University of Reading, Reading RG6 6DX, UKDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, ItalyDepartment of Chemistry, University of Reading, Reading RG6 6DX, UKRapid profiling of the biomolecular components of milk can be useful for food quality assessment and for food fraud detection. Differences in commercial value and availability of milk from specific species are often the reasons for the illicit and fraudulent sale of milk whose species origin is wrongly declared. In this study, a fast, MS-based speciation method is presented to distinguish sheep from goat milk and sheep colostrum at different phases. Using liquid atmospheric pressure (AP)-matrix-assisted laser desorption/ionisation (MALDI) MS, it was possible to classify samples of goat and sheep milk with 100% accuracy in one minute of data acquisition per sample. Moreover, an accuracy of 98% was achieved in classifying pure sheep milk samples and sheep milk samples containing 10% goat milk. Evaluating colostrum quality and postnatal stages represents another possible application of this technology. Classification of sheep colostrum samples that were collected within 6 hours after parturition and 48 hours later was achieved with an accuracy of 84.4%. Our data show that substantial changes in the lipid profile can account for the accurate classification of colostrum collected at the early and late time points. This method applied to the analysis of protein orthologs of different species can, as in this case, allow unequivocal speciation analysis.https://www.mdpi.com/2227-7382/8/3/20goat–sheep speciationcolostrumprotein–proteoform profilingMALDImass spectrometry |
| spellingShingle | Cristian Piras Carlotta Ceniti Evita Hartmane Nicola Costanzo Valeria Maria Morittu Paola Roncada Domenico Britti Rainer Cramer Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis Proteomes goat–sheep speciation colostrum protein–proteoform profiling MALDI mass spectrometry |
| title | Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis |
| title_full | Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis |
| title_fullStr | Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis |
| title_full_unstemmed | Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis |
| title_short | Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis |
| title_sort | rapid liquid ap maldi ms profiling of lipids and proteins from goat and sheep milk for speciation and colostrum analysis |
| topic | goat–sheep speciation colostrum protein–proteoform profiling MALDI mass spectrometry |
| url | https://www.mdpi.com/2227-7382/8/3/20 |
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