Micropropagation of Grapevine and Strawberry from South Russia: Rapid Production and Genetic Uniformity
Grapes and strawberries are major fruit crops with a dynamic, fast-growing global market. In addition to conventional techniques, biotechnological tools allow the preservation of valuable genotypes of both crops and the large-scale propagation of high-quality material. We have developed new protocol...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/12/2/308 |
| _version_ | 1797483739283980288 |
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| author | Lavr A. Kryukov Dmitry I. Vodolazhsky Rina Kamenetsky-Goldstein |
| author_facet | Lavr A. Kryukov Dmitry I. Vodolazhsky Rina Kamenetsky-Goldstein |
| author_sort | Lavr A. Kryukov |
| collection | DOAJ |
| description | Grapes and strawberries are major fruit crops with a dynamic, fast-growing global market. In addition to conventional techniques, biotechnological tools allow the preservation of valuable genotypes of both crops and the large-scale propagation of high-quality material. We have developed new protocols for expeditious and reliable micropropagation of grapes and strawberries cultivated in south Russia. In vitro cultivation on semisolid media was combined with rapid propagation in bioreactors. A six-week cycle of propagation in a bioreactor resulted in a 300-fold increase in fresh mass, with a propagation rate of ~5 in grapes and ~20 in strawberries. Genetic analysis using the inter simple sequence repeat (ISSR) DNA fingerprinting technique confirmed the full uniformity of the regenerants. The results of this research will support the local horticultural industry with fast and efficient propagation systems of new breeding lines, introduced varieties, and elite cultivars. |
| first_indexed | 2024-03-09T22:51:21Z |
| format | Article |
| id | doaj.art-7478b6a448e74e0297c2010f4dbefa35 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T22:51:21Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-7478b6a448e74e0297c2010f4dbefa352023-11-23T18:19:45ZengMDPI AGAgronomy2073-43952022-01-0112230810.3390/agronomy12020308Micropropagation of Grapevine and Strawberry from South Russia: Rapid Production and Genetic UniformityLavr A. Kryukov0Dmitry I. Vodolazhsky1Rina Kamenetsky-Goldstein2Center for Advanced Scientific and Technological Development, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, RussiaCenter for Advanced Scientific and Technological Development, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, RussiaInstitute of Plant Sciences, The Volcani Center, Agricultural Research Organization, Rishon LeZion 7505101, IsraelGrapes and strawberries are major fruit crops with a dynamic, fast-growing global market. In addition to conventional techniques, biotechnological tools allow the preservation of valuable genotypes of both crops and the large-scale propagation of high-quality material. We have developed new protocols for expeditious and reliable micropropagation of grapes and strawberries cultivated in south Russia. In vitro cultivation on semisolid media was combined with rapid propagation in bioreactors. A six-week cycle of propagation in a bioreactor resulted in a 300-fold increase in fresh mass, with a propagation rate of ~5 in grapes and ~20 in strawberries. Genetic analysis using the inter simple sequence repeat (ISSR) DNA fingerprinting technique confirmed the full uniformity of the regenerants. The results of this research will support the local horticultural industry with fast and efficient propagation systems of new breeding lines, introduced varieties, and elite cultivars.https://www.mdpi.com/2073-4395/12/2/308<i>Vitis</i><i>Fragaria</i>ISSRin vitro culture |
| spellingShingle | Lavr A. Kryukov Dmitry I. Vodolazhsky Rina Kamenetsky-Goldstein Micropropagation of Grapevine and Strawberry from South Russia: Rapid Production and Genetic Uniformity Agronomy <i>Vitis</i> <i>Fragaria</i> ISSR in vitro culture |
| title | Micropropagation of Grapevine and Strawberry from South Russia: Rapid Production and Genetic Uniformity |
| title_full | Micropropagation of Grapevine and Strawberry from South Russia: Rapid Production and Genetic Uniformity |
| title_fullStr | Micropropagation of Grapevine and Strawberry from South Russia: Rapid Production and Genetic Uniformity |
| title_full_unstemmed | Micropropagation of Grapevine and Strawberry from South Russia: Rapid Production and Genetic Uniformity |
| title_short | Micropropagation of Grapevine and Strawberry from South Russia: Rapid Production and Genetic Uniformity |
| title_sort | micropropagation of grapevine and strawberry from south russia rapid production and genetic uniformity |
| topic | <i>Vitis</i> <i>Fragaria</i> ISSR in vitro culture |
| url | https://www.mdpi.com/2073-4395/12/2/308 |
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