Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding
Detection of Toxoplasma gondii DNA in cat feces is considered indicative of the presence of T. gondii oocysts. This study aims to demonstrate that the high sensitivity of qPCR can lead to T. gondii DNA detection in cat feces in the absence of oocysts. A cat immune to toxoplasmosis was fed with a mou...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2016-01-01
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| Series: | Parasite |
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| Online Access: | http://dx.doi.org/10.1051/parasite/2016029 |
| _version_ | 1797641343109955584 |
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| author | Poulle Marie-Lazarine Forin-Wiart Marie-Amélie Josse-Dupuis Émilie Villena Isabelle Aubert Dominique |
| author_facet | Poulle Marie-Lazarine Forin-Wiart Marie-Amélie Josse-Dupuis Émilie Villena Isabelle Aubert Dominique |
| author_sort | Poulle Marie-Lazarine |
| collection | DOAJ |
| description | Detection of Toxoplasma gondii DNA in cat feces is considered indicative of the presence of T. gondii oocysts. This study aims to demonstrate that the high sensitivity of qPCR can lead to T. gondii DNA detection in cat feces in the absence of oocysts. A cat immune to toxoplasmosis was fed with a mouse experimentally infected with T. gondii. Detection of DNA of this parasite was performed by qPCR on feces passed: (i) on the day the cat ingested the infected prey; (ii) during the three previous days; and (iii) during the three following days. The kinetics of qPCR results are clearly not linked to oocyst shedding and this result demonstrates that qPCR can detect T. gondii DNA related to bradyzoites from an infected prey, in the absence of oocysts. Caution is thus recommended when interpreting T. gondii qPCR results for samples of cat feces. |
| first_indexed | 2024-03-11T13:44:15Z |
| format | Article |
| id | doaj.art-747a5387a95843eea0cec386c26ec618 |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-11T13:44:15Z |
| publishDate | 2016-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-747a5387a95843eea0cec386c26ec6182023-11-02T11:00:40ZengEDP SciencesParasite1776-10422016-01-01232910.1051/parasite/2016029parasite160040Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst sheddingPoulle Marie-LazarineForin-Wiart Marie-AmélieJosse-Dupuis ÉmilieVillena IsabelleAubert DominiqueDetection of Toxoplasma gondii DNA in cat feces is considered indicative of the presence of T. gondii oocysts. This study aims to demonstrate that the high sensitivity of qPCR can lead to T. gondii DNA detection in cat feces in the absence of oocysts. A cat immune to toxoplasmosis was fed with a mouse experimentally infected with T. gondii. Detection of DNA of this parasite was performed by qPCR on feces passed: (i) on the day the cat ingested the infected prey; (ii) during the three previous days; and (iii) during the three following days. The kinetics of qPCR results are clearly not linked to oocyst shedding and this result demonstrates that qPCR can detect T. gondii DNA related to bradyzoites from an infected prey, in the absence of oocysts. Caution is thus recommended when interpreting T. gondii qPCR results for samples of cat feces.http://dx.doi.org/10.1051/parasite/2016029Toxoplasma gondiiDomestic catOocystCoprodiagnosisqPCR |
| spellingShingle | Poulle Marie-Lazarine Forin-Wiart Marie-Amélie Josse-Dupuis Émilie Villena Isabelle Aubert Dominique Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding Parasite Toxoplasma gondii Domestic cat Oocyst Coprodiagnosis qPCR |
| title | Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding |
| title_full | Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding |
| title_fullStr | Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding |
| title_full_unstemmed | Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding |
| title_short | Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding |
| title_sort | detection of toxoplasma gondii dna by qpcr in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding |
| topic | Toxoplasma gondii Domestic cat Oocyst Coprodiagnosis qPCR |
| url | http://dx.doi.org/10.1051/parasite/2016029 |
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