Identification of Stromal Cells in Spleen Which Support Myelopoiesis
Stromal cells in spleen organize tissue into red pulp, white pulp and marginal zone, and also interact with hematopoietic cells to regulate immune responses. This study has used phenotypic information of a previously described spleen stromal cell line called 5G3, which supports restricted hematopoie...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2019.00001/full |
| _version_ | 1818198904385044480 |
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| author | Hong Kiat Lim Hong Kiat Lim Helen C. O’Neill |
| author_facet | Hong Kiat Lim Hong Kiat Lim Helen C. O’Neill |
| author_sort | Hong Kiat Lim |
| collection | DOAJ |
| description | Stromal cells in spleen organize tissue into red pulp, white pulp and marginal zone, and also interact with hematopoietic cells to regulate immune responses. This study has used phenotypic information of a previously described spleen stromal cell line called 5G3, which supports restricted hematopoiesis in vitro, to identify an equivalent stromal cell subset in vivo and to test its capacity to support hematopoiesis. Using stromal cell fractionation, phenotypic analysis, as well as cell growth and hematopoietic support assays, the Sca-1+gp38+Thy1.2+CD29+CD51+ fraction of spleen stroma has been identified as an equivalent stromal subset resembling the 5G3 cell counterpart. While heterogeneity may still exist within that subset, it has been shown to have superior hematopoietic support capacity compared with the 5G3 cell line, and all other spleen stromal cell fractions tested. |
| first_indexed | 2024-12-12T02:13:17Z |
| format | Article |
| id | doaj.art-747b852bee43458cafb0989a2d2a9773 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-12T02:13:17Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-747b852bee43458cafb0989a2d2a97732022-12-22T00:41:51ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2019-01-01710.3389/fcell.2019.00001425328Identification of Stromal Cells in Spleen Which Support MyelopoiesisHong Kiat Lim0Hong Kiat Lim1Helen C. O’Neill2Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University, Gold Coast, QLD, AustraliaResearch School of Biology, Australian National University, Canberra, ACT, AustraliaClem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University, Gold Coast, QLD, AustraliaStromal cells in spleen organize tissue into red pulp, white pulp and marginal zone, and also interact with hematopoietic cells to regulate immune responses. This study has used phenotypic information of a previously described spleen stromal cell line called 5G3, which supports restricted hematopoiesis in vitro, to identify an equivalent stromal cell subset in vivo and to test its capacity to support hematopoiesis. Using stromal cell fractionation, phenotypic analysis, as well as cell growth and hematopoietic support assays, the Sca-1+gp38+Thy1.2+CD29+CD51+ fraction of spleen stroma has been identified as an equivalent stromal subset resembling the 5G3 cell counterpart. While heterogeneity may still exist within that subset, it has been shown to have superior hematopoietic support capacity compared with the 5G3 cell line, and all other spleen stromal cell fractions tested.https://www.frontiersin.org/article/10.3389/fcell.2019.00001/fullhematopoiesisstromaspleenmyelopoiesismicroenvironment |
| spellingShingle | Hong Kiat Lim Hong Kiat Lim Helen C. O’Neill Identification of Stromal Cells in Spleen Which Support Myelopoiesis Frontiers in Cell and Developmental Biology hematopoiesis stroma spleen myelopoiesis microenvironment |
| title | Identification of Stromal Cells in Spleen Which Support Myelopoiesis |
| title_full | Identification of Stromal Cells in Spleen Which Support Myelopoiesis |
| title_fullStr | Identification of Stromal Cells in Spleen Which Support Myelopoiesis |
| title_full_unstemmed | Identification of Stromal Cells in Spleen Which Support Myelopoiesis |
| title_short | Identification of Stromal Cells in Spleen Which Support Myelopoiesis |
| title_sort | identification of stromal cells in spleen which support myelopoiesis |
| topic | hematopoiesis stroma spleen myelopoiesis microenvironment |
| url | https://www.frontiersin.org/article/10.3389/fcell.2019.00001/full |
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