Evaluation of HiCrome KPC Agar for the Screening of Carbapenem-Resistant Enterobacterales Colonization in the ICU Setting of a Tertiary Care Hospital
Background Spread of carbapenem-resistant Enterobacterales (CRE) is a significant concern in intensive care unit (ICU) settings. Approaches to routine screening for CRE colonization in all ICU patients vary depending on institutional epidemiology and resources. The present study was aimed to evaluat...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Thieme Medical and Scientific Publishers Pvt. Ltd.
2021-07-01
|
| Series: | Journal of Laboratory Physicians |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1732494 |
| _version_ | 1819094092966002688 |
|---|---|
| author | Ashoka Mahapatra K Nikitha Sutapa Rath Bijayini Behera Kavita Gupta |
| author_facet | Ashoka Mahapatra K Nikitha Sutapa Rath Bijayini Behera Kavita Gupta |
| author_sort | Ashoka Mahapatra |
| collection | DOAJ |
| description | Background Spread of carbapenem-resistant Enterobacterales (CRE) is a significant concern in intensive care unit (ICU) settings. Approaches to routine screening for CRE colonization in all ICU patients vary depending on institutional epidemiology and resources. The present study was aimed to evaluate the performance of HiCrome Klebsiella pneumoniae carbapenemase (KPC) agar for the detection of CRE colonization in ICU settings taking the Centers for Disease Control and Prevention (CDC) recommended method as reference.
Methods Two-hundred and eighty rectal swabs (duplicate) from 140 patients were subjected to CRE detection in HiCrome KPC agar and MacConkey agar (CDC criteria).
Results Using CDC method, total 41 CRE isolates were recovered comprising of 29 Escherichia coli, 11 Klebsiella, and 1 Enterobacter spp. On the other hand, 49 isolates of CRE recovered from 140 rectal swabs using HiCrome KPC agar, out of which 33 were E. coli, 15 Klebsiella, and 1 Enterobacter sp.
Statistical Analysis Sensitivity, specificity, negative, and positive predictive values of CRE screening by HiCrome KPC agar were found to be 100% (91.4–100), 91.9% (84.8–95.8), 83.6% (70.9–91.4), and 100% (95.9–100), respectively, taking the CDC recommended method as reference.
Conclusion HiCrome KPC agar has high sensitivity in screening CRE colonization. Further studies are needed to establish its applicability for detecting the predominant circulating carbapenemases in the Indian setting. |
| first_indexed | 2024-12-21T23:21:55Z |
| format | Article |
| id | doaj.art-7480637380a34df89dcb1092ce8ebd07 |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-21T23:21:55Z |
| publishDate | 2021-07-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-7480637380a34df89dcb1092ce8ebd072022-12-21T18:46:46ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262021-07-01130435836110.1055/s-0041-1732494Evaluation of HiCrome KPC Agar for the Screening of Carbapenem-Resistant Enterobacterales Colonization in the ICU Setting of a Tertiary Care HospitalAshoka Mahapatra0K Nikitha1Sutapa Rath2Bijayini Behera3Kavita Gupta4Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Orissa, IndiaAll India Institute of Medical Sciences, Bhubaneswar, Orissa, IndiaDepartment of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Orissa, IndiaDepartment of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Orissa, IndiaDepartment of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Orissa, IndiaBackground Spread of carbapenem-resistant Enterobacterales (CRE) is a significant concern in intensive care unit (ICU) settings. Approaches to routine screening for CRE colonization in all ICU patients vary depending on institutional epidemiology and resources. The present study was aimed to evaluate the performance of HiCrome Klebsiella pneumoniae carbapenemase (KPC) agar for the detection of CRE colonization in ICU settings taking the Centers for Disease Control and Prevention (CDC) recommended method as reference. Methods Two-hundred and eighty rectal swabs (duplicate) from 140 patients were subjected to CRE detection in HiCrome KPC agar and MacConkey agar (CDC criteria). Results Using CDC method, total 41 CRE isolates were recovered comprising of 29 Escherichia coli, 11 Klebsiella, and 1 Enterobacter spp. On the other hand, 49 isolates of CRE recovered from 140 rectal swabs using HiCrome KPC agar, out of which 33 were E. coli, 15 Klebsiella, and 1 Enterobacter sp. Statistical Analysis Sensitivity, specificity, negative, and positive predictive values of CRE screening by HiCrome KPC agar were found to be 100% (91.4–100), 91.9% (84.8–95.8), 83.6% (70.9–91.4), and 100% (95.9–100), respectively, taking the CDC recommended method as reference. Conclusion HiCrome KPC agar has high sensitivity in screening CRE colonization. Further studies are needed to establish its applicability for detecting the predominant circulating carbapenemases in the Indian setting.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1732494crehicrome kpc agarrectal swab |
| spellingShingle | Ashoka Mahapatra K Nikitha Sutapa Rath Bijayini Behera Kavita Gupta Evaluation of HiCrome KPC Agar for the Screening of Carbapenem-Resistant Enterobacterales Colonization in the ICU Setting of a Tertiary Care Hospital Journal of Laboratory Physicians cre hicrome kpc agar rectal swab |
| title | Evaluation of HiCrome KPC Agar for the Screening of Carbapenem-Resistant Enterobacterales Colonization in the ICU Setting of a Tertiary Care Hospital |
| title_full | Evaluation of HiCrome KPC Agar for the Screening of Carbapenem-Resistant Enterobacterales Colonization in the ICU Setting of a Tertiary Care Hospital |
| title_fullStr | Evaluation of HiCrome KPC Agar for the Screening of Carbapenem-Resistant Enterobacterales Colonization in the ICU Setting of a Tertiary Care Hospital |
| title_full_unstemmed | Evaluation of HiCrome KPC Agar for the Screening of Carbapenem-Resistant Enterobacterales Colonization in the ICU Setting of a Tertiary Care Hospital |
| title_short | Evaluation of HiCrome KPC Agar for the Screening of Carbapenem-Resistant Enterobacterales Colonization in the ICU Setting of a Tertiary Care Hospital |
| title_sort | evaluation of hicrome kpc agar for the screening of carbapenem resistant enterobacterales colonization in the icu setting of a tertiary care hospital |
| topic | cre hicrome kpc agar rectal swab |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1732494 |
| work_keys_str_mv | AT ashokamahapatra evaluationofhicromekpcagarforthescreeningofcarbapenemresistantenterobacteralescolonizationintheicusettingofatertiarycarehospital AT knikitha evaluationofhicromekpcagarforthescreeningofcarbapenemresistantenterobacteralescolonizationintheicusettingofatertiarycarehospital AT sutaparath evaluationofhicromekpcagarforthescreeningofcarbapenemresistantenterobacteralescolonizationintheicusettingofatertiarycarehospital AT bijayinibehera evaluationofhicromekpcagarforthescreeningofcarbapenemresistantenterobacteralescolonizationintheicusettingofatertiarycarehospital AT kavitagupta evaluationofhicromekpcagarforthescreeningofcarbapenemresistantenterobacteralescolonizationintheicusettingofatertiarycarehospital |