EO771, the first luminal B mammary cancer cell line from C57BL/6 mice
Abstract Background Despite decades of therapeutic trials, effective diagnosis, many drugs available and numerous studies on breast cancer, it remains the deadliest cancer in women. In order to choose the most appropriate treatment and to understand the prognosis of the patients, breast cancer is di...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | Cancer Cell International |
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| Online Access: | http://link.springer.com/article/10.1186/s12935-020-01418-1 |
| _version_ | 1828445367097622528 |
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| author | Augustin Le Naour Yvonne Koffi Mariane Diab Delphine Le Guennec Stéphanie Rougé Sahar Aldekwer Nicolas Goncalves-Mendes Jérémie Talvas Marie-Chantal Farges Florence Caldefie-Chezet Marie-Paule Vasson Adrien Rossary |
| author_facet | Augustin Le Naour Yvonne Koffi Mariane Diab Delphine Le Guennec Stéphanie Rougé Sahar Aldekwer Nicolas Goncalves-Mendes Jérémie Talvas Marie-Chantal Farges Florence Caldefie-Chezet Marie-Paule Vasson Adrien Rossary |
| author_sort | Augustin Le Naour |
| collection | DOAJ |
| description | Abstract Background Despite decades of therapeutic trials, effective diagnosis, many drugs available and numerous studies on breast cancer, it remains the deadliest cancer in women. In order to choose the most appropriate treatment and to understand the prognosis of the patients, breast cancer is divided into different subtypes using a molecular classification. Just as there remains a need to discover new effective therapies, models to test them are also required. Methods The EO771 (also named E0771 or EO 771) murine mammary cancer cell line was originally isolated from a spontaneous tumour in C57BL/6 mouse. Although frequently used, this cell line remains poorly characterized. Therefore, the EO771 phenotype was investigated. The phenotype was compared to that of MCF-7 cells, known to be of luminal A subtype and to express estrogen receptors, as well as MDA-MB-231 cells, which are triple negative. Their sensitivity to hormonal treatment was evaluated by viability tests. Results The EO771 were estrogen receptor α negative, estrogen receptor β positive, progesterone receptor positive and ErbB2 positive. This phenotype was associated with a sensitivity to anti-estrogen treatments such as tamoxifen, 4-hydroxy-tamoxifen, endoxifen and fulvestrant. Conclusions On account of the numerous results published with the EO771 cell line, it is important to know its classification, to facilitate comparisons with corresponding types of tumours in patients. Transcriptomic and protein analysis of the EO771 cell line classified it within the luminal B subtype. Luminal B cancers correspond to one of the subtypes most frequently encountered in patients and associated with a poor prognosis. |
| first_indexed | 2024-12-10T21:56:37Z |
| format | Article |
| id | doaj.art-7480c2b4d0524e5897610e13625f6c37 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-10T21:56:37Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-7480c2b4d0524e5897610e13625f6c372022-12-22T01:32:01ZengBMCCancer Cell International1475-28672020-07-0120111310.1186/s12935-020-01418-1EO771, the first luminal B mammary cancer cell line from C57BL/6 miceAugustin Le Naour0Yvonne Koffi1Mariane Diab2Delphine Le Guennec3Stéphanie Rougé4Sahar Aldekwer5Nicolas Goncalves-Mendes6Jérémie Talvas7Marie-Chantal Farges8Florence Caldefie-Chezet9Marie-Paule Vasson10Adrien Rossary11Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneHuman Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-AuvergneAbstract Background Despite decades of therapeutic trials, effective diagnosis, many drugs available and numerous studies on breast cancer, it remains the deadliest cancer in women. In order to choose the most appropriate treatment and to understand the prognosis of the patients, breast cancer is divided into different subtypes using a molecular classification. Just as there remains a need to discover new effective therapies, models to test them are also required. Methods The EO771 (also named E0771 or EO 771) murine mammary cancer cell line was originally isolated from a spontaneous tumour in C57BL/6 mouse. Although frequently used, this cell line remains poorly characterized. Therefore, the EO771 phenotype was investigated. The phenotype was compared to that of MCF-7 cells, known to be of luminal A subtype and to express estrogen receptors, as well as MDA-MB-231 cells, which are triple negative. Their sensitivity to hormonal treatment was evaluated by viability tests. Results The EO771 were estrogen receptor α negative, estrogen receptor β positive, progesterone receptor positive and ErbB2 positive. This phenotype was associated with a sensitivity to anti-estrogen treatments such as tamoxifen, 4-hydroxy-tamoxifen, endoxifen and fulvestrant. Conclusions On account of the numerous results published with the EO771 cell line, it is important to know its classification, to facilitate comparisons with corresponding types of tumours in patients. Transcriptomic and protein analysis of the EO771 cell line classified it within the luminal B subtype. Luminal B cancers correspond to one of the subtypes most frequently encountered in patients and associated with a poor prognosis.http://link.springer.com/article/10.1186/s12935-020-01418-1Antineoplastic agentsHormonalBreast neoplasmsMiceinbred C57BLReceptors |
| spellingShingle | Augustin Le Naour Yvonne Koffi Mariane Diab Delphine Le Guennec Stéphanie Rougé Sahar Aldekwer Nicolas Goncalves-Mendes Jérémie Talvas Marie-Chantal Farges Florence Caldefie-Chezet Marie-Paule Vasson Adrien Rossary EO771, the first luminal B mammary cancer cell line from C57BL/6 mice Cancer Cell International Antineoplastic agents Hormonal Breast neoplasms Mice inbred C57BL Receptors |
| title | EO771, the first luminal B mammary cancer cell line from C57BL/6 mice |
| title_full | EO771, the first luminal B mammary cancer cell line from C57BL/6 mice |
| title_fullStr | EO771, the first luminal B mammary cancer cell line from C57BL/6 mice |
| title_full_unstemmed | EO771, the first luminal B mammary cancer cell line from C57BL/6 mice |
| title_short | EO771, the first luminal B mammary cancer cell line from C57BL/6 mice |
| title_sort | eo771 the first luminal b mammary cancer cell line from c57bl 6 mice |
| topic | Antineoplastic agents Hormonal Breast neoplasms Mice inbred C57BL Receptors |
| url | http://link.springer.com/article/10.1186/s12935-020-01418-1 |
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