Phase Imaging of Phosphatidylcholine Bilayer Membranes by Prodan Fluorescence
Prodan (6-propiponyl-2-(<i>N</i>,<i>N</i>-dimethylamino)naphthalene) is well known as a polarity-sensitive fluorescent probe and has a high capability of detecting structural changes occurring within phospholipid bilayer membranes. In this study, we carried out the fluorescen...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Membranes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0375/12/12/1219 |
| _version_ | 1797456394142613504 |
|---|---|
| author | Nobutake Tamai Hitoshi Matsuki Masaki Goto |
| author_facet | Nobutake Tamai Hitoshi Matsuki Masaki Goto |
| author_sort | Nobutake Tamai |
| collection | DOAJ |
| description | Prodan (6-propiponyl-2-(<i>N</i>,<i>N</i>-dimethylamino)naphthalene) is well known as a polarity-sensitive fluorescent probe and has a high capability of detecting structural changes occurring within phospholipid bilayer membranes. In this study, we carried out the fluorescence spectroscopic observation of bilayer phase behavior for a series of symmetric saturated diacylphosphatidylcholines (C<i>n</i>PCs) with different acyl-chain length <i>n</i> (<i>n</i> = 12–15 and 19–22) using Prodan as a membrane probe to confirm the availability of Prodan along with the previous results for the C<i>n</i>PC bilayer membranes (<i>n</i> = 16–18). The results were discussed by constructing spectral three-dimensional (3D) imaging plots for visualizing the change in bilayer phase states with temperature or pressure to verify the functionality of this 3D imaging plot. It was found that the Prodan fluorescence technique is applicable to the detection of the changes in the bilayer phase states of all C<i>n</i>PCs with a few exceptions. One of the most crucial exceptions was that Prodan cannot be used for the detection of the bilayer-gel state of the C21PC bilayer membrane. It was also found that it is only to the C<i>n</i>PC bilayer membranes with <i>n</i> = 15–18 that the 3D imaging plot is adequately and accurately applicable as a useful graphical tool for visually detecting the bilayer phase states. This is a disadvantageous feature of this technique brought about by the high sensitivity of Prodan as a membrane probe. Further detailed studies on the molecular behavior of Prodan will enable us to find a more useful way of utilizing this membrane probe. |
| first_indexed | 2024-03-09T16:06:56Z |
| format | Article |
| id | doaj.art-748672d840264baf861ce390c006d7d8 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T16:06:56Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-748672d840264baf861ce390c006d7d82023-11-24T16:35:30ZengMDPI AGMembranes2077-03752022-12-011212121910.3390/membranes12121219Phase Imaging of Phosphatidylcholine Bilayer Membranes by Prodan FluorescenceNobutake Tamai0Hitoshi Matsuki1Masaki Goto2Department of Bioengineering, Division of Bioscience and Bioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima 770-8513, JapanDepartment of Bioengineering, Division of Bioscience and Bioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima 770-8513, JapanDepartment of Bioengineering, Division of Bioscience and Bioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima 770-8513, JapanProdan (6-propiponyl-2-(<i>N</i>,<i>N</i>-dimethylamino)naphthalene) is well known as a polarity-sensitive fluorescent probe and has a high capability of detecting structural changes occurring within phospholipid bilayer membranes. In this study, we carried out the fluorescence spectroscopic observation of bilayer phase behavior for a series of symmetric saturated diacylphosphatidylcholines (C<i>n</i>PCs) with different acyl-chain length <i>n</i> (<i>n</i> = 12–15 and 19–22) using Prodan as a membrane probe to confirm the availability of Prodan along with the previous results for the C<i>n</i>PC bilayer membranes (<i>n</i> = 16–18). The results were discussed by constructing spectral three-dimensional (3D) imaging plots for visualizing the change in bilayer phase states with temperature or pressure to verify the functionality of this 3D imaging plot. It was found that the Prodan fluorescence technique is applicable to the detection of the changes in the bilayer phase states of all C<i>n</i>PCs with a few exceptions. One of the most crucial exceptions was that Prodan cannot be used for the detection of the bilayer-gel state of the C21PC bilayer membrane. It was also found that it is only to the C<i>n</i>PC bilayer membranes with <i>n</i> = 15–18 that the 3D imaging plot is adequately and accurately applicable as a useful graphical tool for visually detecting the bilayer phase states. This is a disadvantageous feature of this technique brought about by the high sensitivity of Prodan as a membrane probe. Further detailed studies on the molecular behavior of Prodan will enable us to find a more useful way of utilizing this membrane probe.https://www.mdpi.com/2077-0375/12/12/1219diacylphosphatidylcholinesProdan (6-propiponyl-2-(<i>N</i>,<i>N</i>-dimethylamino)naphthalene)bilayer membranephase transitionhigh pressure |
| spellingShingle | Nobutake Tamai Hitoshi Matsuki Masaki Goto Phase Imaging of Phosphatidylcholine Bilayer Membranes by Prodan Fluorescence Membranes diacylphosphatidylcholines Prodan (6-propiponyl-2-(<i>N</i>,<i>N</i>-dimethylamino)naphthalene) bilayer membrane phase transition high pressure |
| title | Phase Imaging of Phosphatidylcholine Bilayer Membranes by Prodan Fluorescence |
| title_full | Phase Imaging of Phosphatidylcholine Bilayer Membranes by Prodan Fluorescence |
| title_fullStr | Phase Imaging of Phosphatidylcholine Bilayer Membranes by Prodan Fluorescence |
| title_full_unstemmed | Phase Imaging of Phosphatidylcholine Bilayer Membranes by Prodan Fluorescence |
| title_short | Phase Imaging of Phosphatidylcholine Bilayer Membranes by Prodan Fluorescence |
| title_sort | phase imaging of phosphatidylcholine bilayer membranes by prodan fluorescence |
| topic | diacylphosphatidylcholines Prodan (6-propiponyl-2-(<i>N</i>,<i>N</i>-dimethylamino)naphthalene) bilayer membrane phase transition high pressure |
| url | https://www.mdpi.com/2077-0375/12/12/1219 |
| work_keys_str_mv | AT nobutaketamai phaseimagingofphosphatidylcholinebilayermembranesbyprodanfluorescence AT hitoshimatsuki phaseimagingofphosphatidylcholinebilayermembranesbyprodanfluorescence AT masakigoto phaseimagingofphosphatidylcholinebilayermembranesbyprodanfluorescence |