Antisense Transcription in Plants: A Systematic Review and an Update on cis-NATs of Sugarcane
Initially, natural antisense transcripts (NATs, natRNAs, or asRNAs) were considered repressors; however, their functions in gene regulation are diverse. Positive, negative, or neutral correlations to the cognate gene expression have been noted. Although the first studies were published about 50 year...
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| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11603 |
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| author | Luciane Santini Leonardo Yoshida Kaique Dias de Oliveira Carolina Gimiliani Lembke Augusto Lima Diniz Geraldo Cesar Cantelli Milton Yutaka Nishiyama-Junior Glaucia Mendes Souza |
| author_facet | Luciane Santini Leonardo Yoshida Kaique Dias de Oliveira Carolina Gimiliani Lembke Augusto Lima Diniz Geraldo Cesar Cantelli Milton Yutaka Nishiyama-Junior Glaucia Mendes Souza |
| author_sort | Luciane Santini |
| collection | DOAJ |
| description | Initially, natural antisense transcripts (NATs, natRNAs, or asRNAs) were considered repressors; however, their functions in gene regulation are diverse. Positive, negative, or neutral correlations to the cognate gene expression have been noted. Although the first studies were published about 50 years ago, there is still much to be investigated regarding antisense transcripts in plants. A systematic review of scientific publications available in the Web of Science databases was conducted to contextualize how the studying of antisense transcripts has been addressed. Studies were classified considering three categories: “Natural antisense” (208), artificial antisense used in “Genetic Engineering” (797), or “Natural antisense and Genetic Engineering”-related publications (96). A similar string was used for a systematic search in the NCBI Gene database. Of the 1132 antisense sequences found for plants, only 0.8% were cited in PubMed and had antisense information confirmed. This value was the lowest when compared to fungi (2.9%), bacteria (2.3%), and mice (54.1%). Finally, we present an update for the cis-NATs identified in <i>Saccharum</i> spp. Of the 1413 antisense transcripts found in different experiments, 25 showed concordant expressions, 22 were discordant, 1264 did not correlate with the cognate genes, and 102 presented variable results depending on the experiment. |
| first_indexed | 2024-03-09T21:39:12Z |
| format | Article |
| id | doaj.art-748759817ab947e8b46a4acbfc498f23 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:39:12Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-748759817ab947e8b46a4acbfc498f232023-11-23T20:36:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191160310.3390/ijms231911603Antisense Transcription in Plants: A Systematic Review and an Update on cis-NATs of SugarcaneLuciane Santini0Leonardo Yoshida1Kaique Dias de Oliveira2Carolina Gimiliani Lembke3Augusto Lima Diniz4Geraldo Cesar Cantelli5Milton Yutaka Nishiyama-Junior6Glaucia Mendes Souza7Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, BrazilLaboratório de Toxinologia Aplicada, Instituto Butantan, São Paulo 05503-900, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, BrazilInitially, natural antisense transcripts (NATs, natRNAs, or asRNAs) were considered repressors; however, their functions in gene regulation are diverse. Positive, negative, or neutral correlations to the cognate gene expression have been noted. Although the first studies were published about 50 years ago, there is still much to be investigated regarding antisense transcripts in plants. A systematic review of scientific publications available in the Web of Science databases was conducted to contextualize how the studying of antisense transcripts has been addressed. Studies were classified considering three categories: “Natural antisense” (208), artificial antisense used in “Genetic Engineering” (797), or “Natural antisense and Genetic Engineering”-related publications (96). A similar string was used for a systematic search in the NCBI Gene database. Of the 1132 antisense sequences found for plants, only 0.8% were cited in PubMed and had antisense information confirmed. This value was the lowest when compared to fungi (2.9%), bacteria (2.3%), and mice (54.1%). Finally, we present an update for the cis-NATs identified in <i>Saccharum</i> spp. Of the 1413 antisense transcripts found in different experiments, 25 showed concordant expressions, 22 were discordant, 1264 did not correlate with the cognate genes, and 102 presented variable results depending on the experiment.https://www.mdpi.com/1422-0067/23/19/11603plantsasRNAcis-NATsystematic reviewartificial antisensetransgenic plants |
| spellingShingle | Luciane Santini Leonardo Yoshida Kaique Dias de Oliveira Carolina Gimiliani Lembke Augusto Lima Diniz Geraldo Cesar Cantelli Milton Yutaka Nishiyama-Junior Glaucia Mendes Souza Antisense Transcription in Plants: A Systematic Review and an Update on cis-NATs of Sugarcane International Journal of Molecular Sciences plants asRNA cis-NAT systematic review artificial antisense transgenic plants |
| title | Antisense Transcription in Plants: A Systematic Review and an Update on cis-NATs of Sugarcane |
| title_full | Antisense Transcription in Plants: A Systematic Review and an Update on cis-NATs of Sugarcane |
| title_fullStr | Antisense Transcription in Plants: A Systematic Review and an Update on cis-NATs of Sugarcane |
| title_full_unstemmed | Antisense Transcription in Plants: A Systematic Review and an Update on cis-NATs of Sugarcane |
| title_short | Antisense Transcription in Plants: A Systematic Review and an Update on cis-NATs of Sugarcane |
| title_sort | antisense transcription in plants a systematic review and an update on cis nats of sugarcane |
| topic | plants asRNA cis-NAT systematic review artificial antisense transgenic plants |
| url | https://www.mdpi.com/1422-0067/23/19/11603 |
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