Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates
Abstract Bifidobacteria are among the first and most abundant bacterial colonizers of the gastrointestinal tract of (breast-fed) healthy infants. Their success of colonising the infant gut is believed to be, at least partly, due to their ability to metabolize available carbon sources by means of sec...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-01-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-019-0735-3 |
| _version_ | 1818338555677638656 |
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| author | Valentina Ambrogi Francesca Bottacini Joyce O’Sullivan Mary O’Connell Motherway Cao Linqiu Barry Schoemaker Margriet Schoterman Douwe van Sinderen |
| author_facet | Valentina Ambrogi Francesca Bottacini Joyce O’Sullivan Mary O’Connell Motherway Cao Linqiu Barry Schoemaker Margriet Schoterman Douwe van Sinderen |
| author_sort | Valentina Ambrogi |
| collection | DOAJ |
| description | Abstract Bifidobacteria are among the first and most abundant bacterial colonizers of the gastrointestinal tract of (breast-fed) healthy infants. Their success of colonising the infant gut is believed to be, at least partly, due to their ability to metabolize available carbon sources by means of secreted or intracellular glycosyl hydrolases (GHs). Among these, β-galactosidases are particularly relevant as they allow bifidobacteria to grow on β-galactosyl-linked saccharidic substrates, which are present in copious amounts in the milk-based diet of their infant host (e.g. lactose and human milk oligosaccharides). In the present study we employed an in silico analysis to identify GH family 2 and 42 β-galactosidases encoded by typical infant-associated bifidobacteria. Comparative genome analysis followed by characterisation of selected β-galactosidases revealed how these GH2 and GH42 members are distributed among these infant-associated bifidobacteria, while their hydrolytic activity towards growth substrates commonly available in the infant gut were also assessed. |
| first_indexed | 2024-12-13T15:12:59Z |
| format | Article |
| id | doaj.art-7488338cd1884871bbc0de923d815fe0 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-13T15:12:59Z |
| publishDate | 2019-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-7488338cd1884871bbc0de923d815fe02022-12-21T23:40:48ZengSpringerOpenAMB Express2191-08552019-01-019111510.1186/s13568-019-0735-3Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolatesValentina Ambrogi0Francesca Bottacini1Joyce O’Sullivan2Mary O’Connell Motherway3Cao Linqiu4Barry Schoemaker5Margriet Schoterman6Douwe van Sinderen7APC Microbiome Ireland, University College CorkAPC Microbiome Ireland, University College CorkAPC Microbiome Ireland, University College CorkAPC Microbiome Ireland, University College CorkFrieslandCampinaFrieslandCampinaFrieslandCampinaAPC Microbiome Ireland, University College CorkAbstract Bifidobacteria are among the first and most abundant bacterial colonizers of the gastrointestinal tract of (breast-fed) healthy infants. Their success of colonising the infant gut is believed to be, at least partly, due to their ability to metabolize available carbon sources by means of secreted or intracellular glycosyl hydrolases (GHs). Among these, β-galactosidases are particularly relevant as they allow bifidobacteria to grow on β-galactosyl-linked saccharidic substrates, which are present in copious amounts in the milk-based diet of their infant host (e.g. lactose and human milk oligosaccharides). In the present study we employed an in silico analysis to identify GH family 2 and 42 β-galactosidases encoded by typical infant-associated bifidobacteria. Comparative genome analysis followed by characterisation of selected β-galactosidases revealed how these GH2 and GH42 members are distributed among these infant-associated bifidobacteria, while their hydrolytic activity towards growth substrates commonly available in the infant gut were also assessed.http://link.springer.com/article/10.1186/s13568-019-0735-3β-GalactosidasesBifidobacteriaLactoseHMOsInfant gut microbiota |
| spellingShingle | Valentina Ambrogi Francesca Bottacini Joyce O’Sullivan Mary O’Connell Motherway Cao Linqiu Barry Schoemaker Margriet Schoterman Douwe van Sinderen Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates AMB Express β-Galactosidases Bifidobacteria Lactose HMOs Infant gut microbiota |
| title | Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates |
| title_full | Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates |
| title_fullStr | Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates |
| title_full_unstemmed | Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates |
| title_short | Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates |
| title_sort | characterization of gh2 and gh42 β galactosidases derived from bifidobacterial infant isolates |
| topic | β-Galactosidases Bifidobacteria Lactose HMOs Infant gut microbiota |
| url | http://link.springer.com/article/10.1186/s13568-019-0735-3 |
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